RESUMO
Objective: To explore the efficacy of earthworm's coelomic fluid against gentamicin-induced hepatic and renal toxicity in rats. Methods: The animals were divided randomly into three groups (n= 6 per group): control, gentamicin, and Allolobophora caliginosa coelomic fluid-treated groups. Toxicity was established after injection of gentamicin daily for 8 days at a dose of 100 mg/kg. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total proteins, albumin, creatinine, urea, uric acid, malondialdehyde, glutathione, catalase and histopathology of tissues were investigated in the study. Results: Allolobophora caliginosa coelomic fluid significantly decreased urea, creatinine, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and malondialdehyde levels while significantly increasing levels of total proteins, albumin, glutathione and catalase. The histopathological investigation showed partial restoration of renal and hepatic architecture. Conclusions: This study shows the potency of Allolobophora caliginosa coelomic fluid in improving the biochemical and histopathological changes induced by gentamicin in the liver and kidney of the rats.
RESUMO
Objective: To explore the efficacy of earthworm's coelomic fluid against gentamicin-induced hepatic and renal toxicity in rats. Methods: The animals were divided randomly into three groups (n = 6 per group): control, gentamicin, and Allolobophora caliginosa coelomic fluid-treated groups. Toxicity was established after injection of gentamicin daily for 8 days at a dose of 100 mg/kg. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total proteins, albumin, creatinine, urea, uric acid, malondialdehyde, glutathione, catalase and histopathology of tissues were investigated in the study. Results: Allolobophora caliginosa coelomic fluid significantly decreased urea, creatinine, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and malondialdehyde levels while significantly increasing levels of total proteins, albumin, glutathione and catalase. The histopathological investigation showed partial restoration of renal and hepatic architecture. Conclusions: This study shows the potency of Allolobophora caliginosa coelomic fluid in improving the biochemical and histopathological changes induced by gentamicin in the liver and kidney of the rats.
RESUMO
The different malignancy advancing systems related with focal adhesion kinase (FAK) can be attentive in theprogression of colorectal cancer. By inhibiting the growth mechanism of mixture of 5-Fluorouracil (5-FU) andearthworm therapy could enhance the affectability of malignant growth cells. The objective of this research was toexplore the antiproliferative activity of mixed coelomic fluid cancer medicines produced from Lumbricus rubellus.Colorectal cancer was actuated in mice by injected HT-29 cells in the caecum of mice, and then 5% dextran sodiumsulfate were given by the drinking water. To investigate the effect of combination treatment, we divided of fivetreatment groups: group 1 was untreated (vehicle), groups 2–5 were given by 5-FU. Groups 3–5 were given by acombination of 5-FU (50 μg/g BW) and coelomic fluid (50, 100, and 200 μg/g BW), respectively. The administratedof coelomic fluid was started from the 4th weeks. Mice were sacrificed at the end of 8 weeks and colon tissue wasisolated, then assessed using Flowcytometry and immunofluorescent. These findings revealed that the mixture of 5FUand coelomic fluid components inhibits growth considerably, Interleukin-1β (IL-1β) and FAK compare with 5-FUonly group (p < 0.001; p < 0.05; and p < 0.001). Treatment used coelomic fluid at a dose of 200 μg/g BW decreasedFAK expression. The combination of 5-FU and coelomic fluid at a dose of 200 μg/g BW decreased the percentage ofIL-1β (p < 0.05).