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Through analyzing the gene expression profile of tumor cells with gene chip technology and se -lecting the differentially expressed genes of different tumor individuals and tumor types ,researchers find that these genes are not only connected with tumor growth ,proliferation,apoptosis,and signal transduction,but also with tumor pathogenesis,tumor infiltration,tumor metastasis and tumor drug resistance .Some tumor differentially ex-pressed genes or genomes show that these genes are even associated with tumor histological source and histological type.It demonstrates practical application value in the pathological diagnosis and differential diagnosis of tumors .
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The aim of this study was to determine the cytomorphological characteristics of circulating tumor cells (CTCS) in patients with colo-rectal cancer and compare them with the primary tumor and metastasis. CTCS were obtained from blood using differential gel centrifugation and detected using standard immunocytochemistry using anti-CEA. Primary CTCs were defined as those detected before surgery and secondary CTCs those detected after. Surgical specimens of the primary tumor and metastasis were evaluated using standard histological methods with hematoxillin and eosin. CTCs both primary and secondary retained the cytomorphological characteristics of the primary tumor, showing marked intra-patient pleomorphism. There were no differences between primary and secondary CTCs in their cytomorphological features. CTCs from patients with signet ring tumors showed the presence of intracellular mucin deposits. Groups of 3 or more CTCs were only seen in patients with metastasis, whereas duplets of CTcs were seen in patients with metastatic and non-metastatic colo-rectal cancer. This study provides an initial analysis of the cytomorphological features of CTCs, providing a foundation for further investigation into the significance and metastatic potential of CTCs...
El objetivo de este trabajo fue determinar las características cito-morfológicas de las células tumorales circulantes (CTCs) en pacientes con cáncer colo-rectal y compararlas con la cito-morfología del tumor primario y de las metástasis. CTCs fueron obtenidas de la sangre venosa usando centrifugación diferencial y detectadas utilizando inmumocitoquímica estándar con un anticuerpo monoclonal contra el antígeno carcino-embrionico. Las CTCs primarias fueron definidas como aquellas detectadas antes de la cirugía, y las CTCs secundarias aquellas detectadas después de la cirugía. Las piezas quirúrgicas fueron analizadas con métodos histológicos estándares con hematoxilina y eosina. Las CTCs primarias y secundarias se mantengan las mismas características cito-morfológicas que el tumor primario, con una grande variabilidad pleomorfica entre los diferentes pacientes. No hubo diferencias entre CTCs primarias y secundarias en términos de su morfología. Las CTCs detectadas en pacientes con tumores tipo anillo en sello tuvieron la presencia de inclusiones de mucina. CTCs agrupadas en 3 o más células solamente fueron detectadas en pacientes con metástasis, mientras la detección de CTCS en grupos de 2 células fueron detectadas en pacientes con o sin metástasis. Este estudio demuestra un análisis inicial de los hallazgos citomorfológicos de las CTCs en pacientes Chilenos con cáncer colo-rectal, y la base para investigaciones futuras acerca la significancia y potencial metastasica de las CTCs...
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Humanos , Células Neoplásicas Circulantes/patologia , Neoplasias Colorretais/patologia , Centrifugação , Chile , Imuno-HistoquímicaRESUMO
PURPOSE: This study was to prepare the basic data for prevention of colo-rectal cancer and protection against its spread. METHOD: The subjects for the study were 204 patients with colo-rectal cancer from the G.S. wards of five general hospitals in Busan for the period of June 1 to September 1, 2001. The instrument used was a questionaire which was developed by Junho Shin(1995). The data was analyzed by descriptive statistics, McNemar test, x2 test, paired t-test, and ANOVA. RESULT: 1. 57% of the studied subjects were male, 42.7% were more than 60 years old, 54.9% were of standard physique, 49.5% were the Buddhists, 50.5% were the middle or high school graduates, 83.8% were city dwellers, 48% were jobless, 69.6% had no-history of alimentary disease, 44.1% had no-family history of cancer, 82.8% were married. 2. Constipation(x2=36.45, p=0.0001) in clients showed a significant positive association for the pre- diagnosed, but diarrhea(x2=3.947, p=0.047) showed a significant positive association for the post-diagnosed. The preference for high seasonings(t=6.23, p=0.0001) and animal fat (t=8.35, p=0.0001) was higher significantly in the pre-diagnosed, but physical activities(x2= 30.22, p=0.0001), an eutrophic or tonic medicine(x2=30.22, p=0.0001), was of and vegetables(t=-6.20, p=0.0001) was higher significantly in the post-diagnosed. 3. There were significant differences in the life style of the pre-diagnosed according to the general characteristics except religion. CONCLUSION: Results of the above study reveal we should be very sensitive to the change of defecation styles and consider the necessity of improving life styles as regards eating habits. When an education program is developed, the general characteristics of the subjects need to be considered.
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Animais , Humanos , Masculino , Pessoa de Meia-Idade , Defecação , Ingestão de Alimentos , Educação , Hospitais Gerais , Estilo de VidaRESUMO
PURPOSE: We studied the effectiveness and toxicities of 5-fluorouracil+leucovorin, combination chemotherapy in advanced or recurred colo-rectal cancer patients, who didn't have previous chemotherapy and enrolled from August 1993 to July 1998. MATERIALS AND METHODS: All patients were treated with leucovorin followed by 5-fluorouracil for 5 consecutive days every 4 weeks. Among 43 patients who were enrolled, 40 patients received treatment at least 2 courses, and they were evaluable. Male to female ratio was 21 to 19. In serum CEA level, 27 patients were greater than 5 ng/ml and 13 were less than 5 ng/ml. And primary site was colon in 21 patients and rectum in 19 patients. RESULTS: The complete response rate was 7,5% and the partial response rate was 25%. The median survival duration was 14.7 months, the median response duration was 16.0 months, and median time to progression was 7.3 months. In the analysis of response, survival duration, time to progression according to various characteristics of patients, serum CEA level and liver involvement were revealed significant difference in survival duration, time to progression (p=0.0122, 00350 & 0.0202, 0.0123) on univariate analysis, but no significant difference on multivariates. Hematologic and non-hematologic toxicities were mild and tolerable. CONCLUSION: This study indicates that the combination of 5-fluorouracil (370 mg/m) and leucovorin (20 mg/m) is effective and tolerable regimen in advanced or recurred colo-rectal cancer patients without previous chemotherapy.