RESUMO
Objective To investigate the characteristics of materiome release kinetics of Liuwei Dihuang Pills (LDP), and to evaluate its visualization. Methods According to the 2015 edition of Chinese Pharmacopia, using the evaluation methods of release kinetics of Chinese material medica to determine the materiomics release kinetics and the releasecharacteristic of LDP by the paddle method. Then f2 fit factor was calculated and evaluated on other types of LDP in comparison with water-bindered pills and condensed pills as, which are two most common preparations in the market. Moreover, materiome release spectrum and release increment spectrum were used, hoping to quantify, integrate and visualise the materiome release characteristics of different types of LDP. Results The kinetic characteristics of the LDP accord with the Weibull release model, T50 and Td were calculated, and there are significant differences between different types of pills (P < 0.05) in addition to condensed pills and water pills. Also, some differences and similarities in the materiomics release characteristic by f2 fit factor were found in the study. Materiome release spectrum and release increment spectrum can be used to quantify, integrate and visualise the materiome release characteristics of LDP on different types. Conclusion The materiomics release kinetics can be applied to quantify, integrate and visualise the materiome release characteristic of LDP on different types. Basically in agreement with the saying, “water pills from the facilitation, honey pills take the slow, paste pills from the late of, wax-wrapped pills take the difficult”.
RESUMO
OBJECTIVE:To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).METHODS:HPLC method was adopted.The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitfile-0.05％ phosphate solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:The linear range ofliquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2).The limits of quantitation were 0.2,0.3 μg/mL,and the limits of detection were 0.1,0.01 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The average recoveries were 98.9％-101.2％ (RSD=0.62％,n=9),98.6％-101.5％ (RSD=1.06％,n=9).CONCLUSIONS:The method is simple and accurate,and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).