RESUMO
Objective: To prepare microspheres immobilized snailase, then optimize the method for making microspheres immobilized snailase and the process conditions for transformation of icariin. Methods: The snailase was embedded in barium-alginate by the method of microemulsion-coacervation. The preparation process was optimized by orthogonal test. Moreover, to optimize and compare the conditions for transforming icariin, such as temperature, pH values, ratio of substrate, and concentration, and to investigate the properties of snailase. Results: Sodium alginates (1.0%), BaCl2 (1.0%), and emulgator were used for making microspheres immobilized snailase by emulsified and immobilized method. Compared with the free snailase, the stability of temperature and pH value of microspheres immobilized snailase was improved. The optimized conditions of microspheres immobilized snailase were 50℃, pH 5, microspheres immobilized snailase mass ratio of 3:1, icariin concentration of 0.4 mg/mL, conversion 2 h, conversion 3 times, transformational ratio up to (53.84±3.41)%. Conclusion: Microspheres immobilized snailase is stable and reusable. Besides, the process of transform icariin is moderate, so it is important and fundamental for industrialization.