Effect and Mechanism of Cortex Lycii Radicis Extracts on the Proliferation and Apoptosis of Pancreatic Beta Cell / 浙江中医药大学学报

Objective] Observing Cortex Lycii Radicis' effect(Cortex Lycii) on rat insulinoma cells(INS-1) proliferation and apoptosis. To explore the mechanism of Cortex Lycii on Pancreatic Beta Cell Proliferation and apoptosis.[ Methods] After primary culture, cells were randomly divided into blank control group:control,11.1mmol·L-1 glucose,HG,30mmol·L-1 glucose, HG+Cortex Lycii(1g·L-1), HG+Cortex Lycii(2g·L-1), HG+Cortex Lycii(4g·L-1), the survival rate of cells was observed by cell counting kit-8(CCK-8);the apoptosis rate was observed by Annexin V stammg. [Results] ①Compared with HG the better effect of cell proliferation groups of Cortex Lycii(P<0.01), the best is group of Cortex Lycii(2g·L-1)(P<0.05) ②Compared with HG group the higher survival rate is group of Cortex Lycii(P<0.01), the lower apoptosis rate of Cortex Lycii(2g·L-1) compared with Cortex Lycii(1g·L-1). [Conclusion] Cortex Lycii can promote the proliferation of pancreatic beta cell, inhibit the apoptosis to protect the pancreatic beta cell. The optimal concentration is 2g·L-1.

"Female Menopathy of ""Phlegm Constitution"" in ""Bamboo Female Subjects Treatment""" / 浙江中医药大学学报

Objective] To explore the Bamboo Gynecology Treatment on the physical phlegm menstrual characteristics of the pathogenesis of various diseases, medication and other aspects of the experience of academic value of the Qing Dynasty. [Method] The history, philology of research methods to collectbamboo Gynecology Treatmenttheories because ofphlegmleading to irregular menstruation, article of its contribution to thephysical phlegmmenopathy. [Results] Bamboo Female Subjects Treatment elaborates the physical phlegm menopathy unique pathogenesis, its emphasis on physical discussed acquired, qi stagnation is considered physical phlegm menopathy pathogenic the key factor; clinical actual situation partition menstrual, prescription flexible, good work, often Erchen based side, a variety of physical phlegm syndromes treating menopathy. Flexible Choice Digupi medication to treat a variety of phlegm types menopathy; at the same time, its emphasis on prescription drugs concocts to enhance clinical efficacy. [Conclusion]Bamboo Female Subjects Treatment is an influential, elaborate physical phlegm and menopathy formation mechanism and other aspects of treatment have more insight for future generations of physicians on the rule, providing emmeniopathy solid theoretical support.

Hypoglycemic Effect of Cortex Lycii Radicis (CLR) on Alloxan induced Diabetic Mice / 中成药

Objective:To observe the hypoglycemic effects of Cortex Lycii Radicis (CLR) on alloxan induced diabetic mice.Methods:The alloxan induced diabetic mice were given drug decoction of CLR[2.5g/(kg?d) -1 , or 5.0g/(kg?d) -1 )] for successive 2 weeks, The blood sugar level was determined with GOD method.Results:There were remarkedly differences in the levels of blood sugar between before and after treatment of various groups. ( P

TLC and HPLC quantitative determination for Xiao'er Ganmao Granules / 中成药

AIM: To establish a method for the quality control of Xiao'er Ganmao Granules(Flos Chrysanthemi, Radix Cynanchi Atrati, Cortex Lycii, etc.). METHODS: Radix Cynanchi Atrati, Cortex Lycii and Flos Chrysanthemi were identified by TLC. The forsythin content in Xiao'er Ganmao Granules was determinated by HPLC. RESULTS: The methodological study showed that a good linear correlation for the forsythin existed in a range of 4.76～588ng. The average recovery of forsythin ( n =9) was 98.7% and RSD was 1.7%. CONCLUSION: The method is simple, accurate and with good repeating and high precision, and can be used for quality control of Xiao'er Ganmao Granules.