Study on the function of the cryopreserved vascular endothelium / 中国现代普通外科进展

Objective:We wanted to design an adequate method with which to cryopreserve rat aorta.Methods:Rat aorta were cryopreserved at 0.7℃/min,and kept frozen for 3 months.After being thawed,arteries were examined for contraction and vasodilation.Results:The mean contractions to KCL,PE,ET-1 and the relaxant ratio of SNP were better preserved in Group B than in Group C(P0.05).Conclusion:A modified cryopreservation technique can better preserves endothelial contractile function and endothelium independent vasodilation of the rat aorta than the conventional method,but the endothelium dependent vasodilation is badly preserved after the cryopreservation.