Development and Validation of HPLC-PDA Method and Pattern Recognition Analyses Using Eight Marker Compounds for the Quality Control Between the Seeds of Cuscuta chinensis Lam. and Cuscuta japonica Choisy

Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

Comparative Study on Chemical Compositions between Cuscuta Japonica and Cuscuta Chinensis Lam / 中成药

The comparative study on the chemical components of Cuscuta japonica and Cuscuta chinensis Lam have been carried out with TLC, paper chromatography and HPLC. As a result, both possessed similar chemical components and same polysaccharide compositions. But the polysaccharide recovery of Cuscuta japonica is three times as high as that of Cuscuta chinensis Lam. Both of them have 16 kinds, of amino acids. The content of 9 kinds of amino acids of Cuscuta japonica was higher than that of Cuscuta chinensis. These findings suggested that the developement and utilization of resources of Cuscuta japonica be available.