RESUMO
The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 ºC, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations.
RESUMO
Aim: In the current investigation thirty four A. flavus cultures were isolated from indoor air samples of some farmers' house in coastal region of Odisha. Production of aflatoxin B1 and the pattern of formation of sclerotia by the isolates were studied in vitro. Methodology: Morphological study and production of sclerotia were carried out by observing fungal colonies on culture media. Conidiophores were identified under Phase contrast microscope. Detection of aflatoxin B1 was done by Thin Layer Chromatography technique. Results: The isolates exhibited variation in their colony characteristics and pattern of sclerotia formation. Overall 44% A. flavus isolates produced stable aflatoxin B1 and 41% were producers of sclerotia. About 32% A. flavus isolates produced both sclerotia and aflatoxin B1. Most of the non toxigenic isolates failed to form thick mycelia mat on β-CD-PDA media. Aflatoxin and sclerotia producing isolates were found more in coastal districts than inter-coastal regions. No significant variation in the pattern of sclerotia production was observed among isolates in less aerobic (sealed plates) and aerobic (unsealed plates) condition, however isolates had slightly more preference towards less aerobic environment for sclerotia production. The size of sclerotia was found to be inversely related to number of sclerotia. Interpretation: The presence of diverse A. flavus strains in farmers' indoor air, having the ability to produce either aflatoxin or sclerotia or both, may lead to serious health implications. This issue need to be addressed and a suitable way for proper management of indoor environment of farmers' need to be designed.
RESUMO
Motion sickness is a very common disturbance of the inner ear that is caused by repeated motion such as from the swell of the sea, the movement of a car, the motion of a plane in turbulent air, etc. Most medications for motion sickness need to be taken at least 30 minutes prior exposure to the activity that can cause the problem. This project is based on the hypothesis that Mouth Dissolving Films (MDF) are rapidly dissolving dosage forms which when placed in the mouth release the drug immediately. These dosage forms would be preferred by pediatric and geriatric patients since these are not associated with fear of choking. The fast dissolving films prepared by solvent casting method with suitable appearance, mechanical strength, peelability and disintegration time were obtained using Methocel E-5 as a primary film former. Meclizine HCl, a poorly water soluble and bitter drug could be successfully incorporated in the fast dissolving films with the help of solubilizers such as β-Cyclodextrine and PEG-400.
RESUMO
PVP only does not enhance the solubility of DHEA but a combination effect will manifeste once the substance is used concurrently with HPCD. In 5,59% HPCD solution, this solubility was enhanced by 115 times and in 5,59% HPCD solution adding 0,1% PVP it was enhanced by 139 times. There is a strict interaction between its components, different evidently from the physical mixed graphic