RESUMO
The present study was designed to further investigate the C steroidal glycosides in Cynanchum plants. Two new steroidal glycosides based on a 13, 14:14, 15-disecopregnane-type aglycone, komaroside P (1) and komaroside Q (2), together with three known compounds (3-5) were isolated from the whole herbs of Cynanchum komarovii. The aglycones of compounds 1 and 2 were two new disecopregnane. Their structures were elucidated on the basis of 1D, 2D NMR spectroscopic data and acid hydrolysis. All the compounds (1-5) showed potent inhibitory activities against human leukemia cell lines (HL-60) with IC values ranging from 16.6 to 26.3 μmol·L, compared to the positive control 5-fluorouracil (6.4 μmol·L).
Assuntos
Humanos , Sobrevivência Celular , Cynanchum , Química , Medicamentos de Ervas Chinesas , Química , Farmacologia , Glicosídeos , Química , Farmacologia , Células HL-60 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Esteroides , Química , FarmacologiaRESUMO
The present study was designed to further investigate the C steroidal glycosides in Cynanchum plants. Two new steroidal glycosides based on a 13, 14:14, 15-disecopregnane-type aglycone, komaroside P (1) and komaroside Q (2), together with three known compounds (3-5) were isolated from the whole herbs of Cynanchum komarovii. The aglycones of compounds 1 and 2 were two new disecopregnane. Their structures were elucidated on the basis of 1D, 2D NMR spectroscopic data and acid hydrolysis. All the compounds (1-5) showed potent inhibitory activities against human leukemia cell lines (HL-60) with IC values ranging from 16.6 to 26.3 μmol·L, compared to the positive control 5-fluorouracil (6.4 μmol·L).
Assuntos
Humanos , Sobrevivência Celular , Cynanchum , Química , Medicamentos de Ervas Chinesas , Química , Farmacologia , Glicosídeos , Química , Farmacologia , Células HL-60 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Esteroides , Química , FarmacologiaRESUMO
Objective To provide the initiative experimental reference for resource utilization of Cynanchum komarovii by biological technology. Methods Using root, stem, and leaf of C. komarovii as explants, the proper media for inducing of callus were optimized by orthogonal test. In culturing process for proliferation of callus, the fresh and dry weights were determined at various times and callus growth curve was drawn. Meanwhile, division test of callus was taken. Results Root callus test showed the best effect in successful inducing percentage of 90%-100%. The best media combination of stem and leaf were MS+2,4-D 1.0 mg/L+6-BA 2.0 mg/L+NAA 0.5 mg/L and MS+2,4-D 0.5 mg/L+6-BA 0.5 mg/L+NAA 0.5 mg/L. The stem segament was more easily induced than leaf. The callus induced by various treatments is in loose character and fresh yellow colour with no brown phenomenon. At day 10 the growth entered the exponent period of callus offspring, the maximal fresh weight to 4.961 g and the dry weight to 0.496 g. The division rate of callus was rather low and not easy to shoot. However, the nail root was commonly seen in the test. Conclusion The root is proved to be the best explants in the test of inducing callus, which shows the best medium of stem and leaf for forming the callus. The growth of callus is bigger, but the division is not easy.