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Objective:To study the effects of IGF-1 on the proliferation,total protein amount and cytodifferentiation of mouse dental follicle cells.Methods:The dental follicle cells of passage 3 were incubated with different concentrations((0.005),0.01,0.05,0.1 and 0.5 mg/L)of IGF-1 respectively,cell proliferation,alkaline phosphatase(ALP),total protein amount were measured by MTT assay and spectrophotometer respectively.Effects of 0.05 and 0.1 mg/L IGF-1 on mineralization potential were studied by Von Kossa staining.Results:IGF-1(ml/L) at 0.005~0.1 increased the proliferation and total protein amount(P
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Objective To explore the expression of MUC-1 (one mucin) in the gastric mucosa of human fetus at different developmental stages and elucidate the relationship between MUC-1 expression and the cytodifferentiation of gastric epithelia. Methods HE staining and immunohistochemistry methods were used. Results The differentiation of the fetal gastric epithelial cells could be divided into three stages, the undifferentiated (S1), the differentiating (S2), and the growing stages (S3). MUC-1 appeared in cell apex of all undifferentiated cells in S1. In S2, MUC-1 appeared in cell apex and cytoplasm in the differentiating cuboidal epithelial cells.In the S3, MUC-1 focused on the glands, especially in the basal cells of glands.Conclusion There was different expression of MUC-1 at various developmental stages of gastric epithelia.