Comparative Study on Detection of Peritoneal Free Cancer Cells of Gastric Surgery / 中国普外基础与临床杂志

Objective To investigate the detection of peritoneal free cancer cells and its clinical significance. Methods The peritoneal free cancer cells,the positive rates of CK20 protein and CK20 mRNA expressions of peritoneal lavage fluid were detected by peritoneal lavage cytology (PLC),flow cytometry (FCM) and real-time fluorescent quantitative RT-PCR in 50 cases of gastric cancer patients,respectively. The sensitivity of three kinds of detection method to peritoneal free cancer cells was compared. Results The positive rates of peritoneal free cancer cells,CK20 protein and mRNA expression of peritoneal lavage fluid were 20.0% (10/50),36.0% (18/50) and 58.0% (29/50),respectively. The positive rate of CK20 mRNA expression detected by real-time fluorescencequantitative RT-PCR in peritoneal lavage fluid was significantly higher than those of the CK20 protein expression detected by FCM and peritoneal free cancer cells detected by PLC (P0.05). The positive rate of CK20 mRNA expression of peritoneal lavage fluid was related to the tumor invasion depth,differentiation degree,TNM stage,and lymph node metastasis (P

Clinical Evaluation of Women with Low Grade Squamous Intraepithelial Lesion on Pap Smears / 대한부인종양콜포스코피학회잡지

The purpose of this study was for clinical evaluating those women with low grade squamous intraepithelial lesions (LSIL) who have been detected on Pap smears. We analyzed 279,270 Pap smears, from January 1994 to August 1997, of which 703 cases were identified as LSIL, and their medical records were reviewed retrospectively. Among them, 616 cases were able to follow-up by altered methods (repeated Pap smear only vs. histologic examination) and their efficacy for detecting more significant lesion (high grade squamous intraepithelial lesion: HSIL or invasive cancer) were also compared. The results were as follows; 1. The frequency of LSIL on Pap smears was approximately 0.25%. 2. The mean age was 39 years (range 18 ~70 years). 3. Most of the gross finding of the cervix were normal or mild erosion. 4. Most of symptom was asymptomatic, or nonspecific. 5. Eighty-seven women with LSIL on initial Pap smears, have performed repeated Pap smears. 74 (85.1%) was normal, 7 (8.0%) was ASCUS, 6 (6.9%) was LSIL. Remained 529 women had subsequently histologic examination such as colposcopic directed biopsy or cone knife biopsy. These histologic results showed 192 (36.3%) with normal, 258 (48.8%) with LSIL, 77 (14.6%) with HSIL, 2 (0.4%) with microinvasive carcinoma. Based on the results in this study, we emphasize the importance of regular screening procedures for early detection of cervical lesions because there was no specific clinical characteristics in women with cytologic diagnosis of LSIL. In addition, we recommened colpo-scopic directed biopsy or cone knife biopsy as follow-up evaluation method in women with LSIL on initial Pap smear for detecting more significant cervical lesion.

Clinical Evaluation of Women with Low Grade Squamous Intraepithelial Lesion on Pap Smears / 대한부인종양콜포스코피학회잡지

The purpose of this study was for clinical evaluating those women with low grade squamous intraepithelial lesions (LSIL) who have been detected on Pap smears. We analyzed 279,270 Pap smears, from January 1994 to August 1997, of which 703 cases were identified as LSIL, and their medical records were reviewed retrospectively. Among them, 616 cases were able to follow-up by altered methods (repeated Pap smear only vs. histologic examination) and their efficacy for detecting more significant lesion (high grade squamous intraepithelial lesion: HSIL or invasive cancer) were also compared. The results were as follows; 1. The frequency of LSIL on Pap smears was approximately 0.25%. 2. The mean age was 39 years (range 18 ~70 years). 3. Most of the gross finding of the cervix were normal or mild erosion. 4. Most of symptom was asymptomatic, or nonspecific. 5. Eighty-seven women with LSIL on initial Pap smears, have performed repeated Pap smears. 74 (85.1%) was normal, 7 (8.0%) was ASCUS, 6 (6.9%) was LSIL. Remained 529 women had subsequently histologic examination such as colposcopic directed biopsy or cone knife biopsy. These histologic results showed 192 (36.3%) with normal, 258 (48.8%) with LSIL, 77 (14.6%) with HSIL, 2 (0.4%) with microinvasive carcinoma. Based on the results in this study, we emphasize the importance of regular screening procedures for early detection of cervical lesions because there was no specific clinical characteristics in women with cytologic diagnosis of LSIL. In addition, we recommened colpo-scopic directed biopsy or cone knife biopsy as follow-up evaluation method in women with LSIL on initial Pap smear for detecting more significant cervical lesion.