RESUMO
Background: The aim of this study is to make a detailed cytological study of effusion fluids and compare with cell block study of the representative cases and IHC studies were done.Methods: Prospective study of 216 cases effusion fluids from in and around hospitals, Mangalore. This study conducted over a period of 18 months from October-2014 to April-2016. This study scrutinized and approved by Institutional Ethics Committee. The samples were processed by conventional cytology using Papanicolaou-stain and Cell Block (CB) method using 10% Alcohol-formalin fixative and stained with H and E. The cellularity, architectural patterns, morphological details were studied both smears. Ancillary immunohistochemical staining with calretinin and EMA are done.Results: A total of 216 cases of effusion fluids with cell block study were included, age range of 13 years to 93 years. Pleural fluid comprised of 55.09%, peritoneal fluid of 43.51% and pericardial fluid of 1.38%. 71% were clinically diagnosed as non-neoplastic and 29% were neoplastic condition. In CS study, 84.5% cases were benign/reactive effusion and 8.5% were positive for malignancy. In CB study, 84.5% were benign/reactive effusion and 10.2% were positive for malignancy. In comparison authors found an increase in diagnostic efficacy by 18%. IHC EMA for adenocarcinoma cells has sensitivity of 100% and calretinin for reactive mesothelial cells has specificity of 100%.Conclusions: Authors concluded that cell block technique when used as an adjuvant to routine smear examination in effusion fluids has increased the diagnostic yield and better preservation of architectural pattern. IHC is helpful in differentiating between reactive mesothelial and adenocarcinoma cells.
RESUMO
Se realizó un estudio descriptivo y retrospectivo de 738 pacientes con lesiones premalignas o malignas del cuello uterino, diagnosticados mediante biopsias y estudios citológicos archivados en fichas en el Departamento de Anatomía Patológica del Hospital General Docente "Dr. Juan Bruno Zayas Alfonso" de Santiago de Cuba, durante el bienio 2011-2012, con vistas a determinar algunas características de dichas formaciones neoplásicas. En la serie se obtuvo que más de la mitad de las muestras hísticas cervicouterinas mostraran alguna lesión premaligna o maligna, predominantemente en pacientes con edades de 25 a 54 años, y el diagnóstico más frecuente fue la displasia moderada; de igual modo, el virus de papiloma humano estuvo presente en 64,8 % de los estudios citológicos y en 75,3 % de las biopsias, y la efectividad del estudio citológico en el laboratorio fue de 92,0 %.
A descriptive and retrospective study of 738 patients with premalignant and malignant lesions of cervix, diagnosed by means of biopsies and cytological studies preserved from records in the Pathology Department of "Dr. Juan Bruno Zayas Alfonso" Teaching General Hospital in Santiago de Cuba was carried out during the biennium 2011-2012, with the aim of determining some characteristics of these neoplastic disorders. In the series it was obtained that more than half of the tissue cervical samples showed some premalignant and malignant lesion, predominantly in patients with ages from 25 to 54 years, and the most frequent diagnosis was moderate dysplasia; likely, the human papillomavirus was present in 64.8% of the cytological studies and in 75.3% of the biopsies, and the effectiveness of the cytological study in the laboratory was 92.0%.