Genetic Polymorphism of Y Chromosome Haplogroup D-M174 in East Asian Populations / 法医学杂志

Objective To explore the genetic polymorphism of Y chromosome D-M174 haplogroup and sub-haplogroups in East Asia. Methods The samples of 1 426 unrelated male individuals from East Asia were collected, and then 7 Y chromosome haplogroup D-M174 and the Y-SNP of its sub-haplogroups were detected with mini-sequencing. The 22 Y-STR genotypes were detected with DNA Typer™ Y26 kit. The haplogroup was analyzed using direct counting method, heatmap, phylogenetic cluster and network graph cluster, and then distribution of genetic polymorphism and the clustering relation between populations and samples of Y chromosome D haplogroup were discussed. Results Haplogroup D-M174 were distributed mostly among Tibetans （40.96%）and Japanese （35.71%）, while less or none were distributed among the surrounding areas of Tibet and other areas. Conclusion The geographical distribution of Y chromosome D-M174 haplogroup in East Asian populations has significant characteristics.

C-peptide and cardiovascular system.

Background: C-peptide and insulin are secreted by pancreatic beta cells in equimolar concentrations. C- Peptide is associated with increased risk of cardiovascular disease. Methods: Routine outpatient department patients were investigated for serum C-peptide, Diabetes, Ischemic heart disease, hypertension and skin lesion acanthosis negrican. Results: Total ninety five patients enrolled in study of these peptide level 2, 2 to5 and >5 found in 22,46,27 patients respectively, with diabetes mellitus in 21 (95%), 39(55%) and 24 (89%), ischemic heart diseases 1(5%), 15(33%), 13(48%) , hypertension 9(41%), 20(44%), 12(45%), acanthosis negrican 9 (41%), 27 (59%), 21 (78%), family history 1 (5%), 18 (39%), 11 (41%) respectively. Conclusion: Significant incidence of hypertension and ischemic heart disease is related to raised level of circulating C-peptide