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1.
Journal of Medical Research ; : 81-85, 2003.
Artigo em Vietnamita | WPRIM | ID: wpr-4955

RESUMO

The study of blood samples extracted directly from healthy people, who is Vietnamese and not relative, lives over the country. PCR technique and denaturing polyacrylamid gel electrophoresis were applied for determination of allele frequencies of D13S317. The primary results showed that allele frequencies of D13S317 locus was polymorphic AND, repetitive sequence unit of 4 nito- base which located on the long hand of chromosome No 13. The locus contained 9 alleles, 242-270 bp. The numbers of repeat units in population were 7, 8, 9, 10, 11, 12, 13, 14, 15. The highest frequency at allele No 8, and allele No 7 had not appear in the observed samples.


Assuntos
População , Frequência do Gene
2.
Korean Journal of Legal Medicine ; : 17-28, 2001.
Artigo em Coreano | WPRIM | ID: wpr-140125

RESUMO

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5%polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 0.85 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65x10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here proved to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.


Assuntos
DNA , Eletroforese , Frequência do Gene , Variação Genética , Células Germinativas , Repetições de Microssatélites , Reação em Cadeia da Polimerase , Coloração pela Prata
3.
Korean Journal of Legal Medicine ; : 17-28, 2001.
Artigo em Coreano | WPRIM | ID: wpr-140124

RESUMO

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5%polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 0.85 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65x10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here proved to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.


Assuntos
DNA , Eletroforese , Frequência do Gene , Variação Genética , Células Germinativas , Repetições de Microssatélites , Reação em Cadeia da Polimerase , Coloração pela Prata
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