RESUMO
【Objective】To investigate the effects of Dan Zhi Xiaoyao San(DZXS) and its extracts on hypothalamic corticotropin releasing hormone(CRH) and plasma corticosterone(CORT) in rats with chronic psychological stress.【Methods】Wistar rats were randomized into 6 groups: A(normal control),B(model control),C (extract of DZXS extracted by water extraction and alcohol precipitation,3.608?g/kg),D(extract of DZXS extracted by petroleum ether,0.087?g/kg),E(extract of DZXS extracted by polysaccharide,2.20?g/kg) and F(DZXS 14.4?g/kg).Except group A,the rat models of chronic multi-phase psychological stress were established in other groups by improved Cart method.Groups A and B were given normal saline,and groups C,D and E were given the corresponding drugs according to the experimental design.Hypothalamic CRH and plasma CORT contents were examined to explore the therapeutic mechanism.【Results】Compared with group A,hypothalamic CRH and plasma CORT contents were increased in group B;hypothalamic CRH content was decreased in groups C,E and F(P
RESUMO
【Objective】To investigate the antianxietic action of Xiaoyao San(XS)and Dan Zhi Xiaoyao San(DZXS).【Methods】 Wistar rats were randomized into 5 groups: model control group,high-and low-dose DZXS groups(14.4?g/kg and 7.2?g/kg respectively),and high-and low-dose XS groups(21.06?g/kg and 10.53g/kg respectively).Social interaction test and open-field test were carried out to observe the antianxietic action of XS and DZXS.Except that the model group was given normal saline,the rats in other groups were given the corresponding drugs according to the experimental design for 14 successive days.After treatment,the activities within 5 min were monitored by two observers and then the mean value were calculated.【Results】High-and low-dose DZXS prolonged rats social interaction time and increased the times of body erection and dressing their hair,and low-dose XS also increased the times of body erection and dressing their hair(P