Sentidos sobre o adoecer por câncer no filme Aquarius / Meanings about cancer in the movie Aquarius / Significations du cancer dans le film Aquarius / Sentidos sobre el cáncer en la película Aquarius

A linguagem fílmica constitui um importante recurso para o ensino-aprendizagem no campo da saúde. Para trabalhar a temática do adoecimento por câncer, diversos filmes têm sido empregados como disparadores de reflexões necessárias à formação de profissionais de saúde capazes de uma atuação mais empática, sensível e humanizada. Para contribuir com esse cenário, o presente estudo teve por objetivo discutir os sentidos sobre o adoecimento pelo câncer a partir da análise do filme Aquarius. Os sentidos predominantes neste filme referem-se ao câncer como uma invasão do corpo sadio e como roubo de algo importante ao sujeito. As temáticas da sexualidade e do protagonismo feminino entrelaçam-se na costura de um filme que metaforiza o câncer em suas múltiplas representações sociais, abrindo espaço para o sentido de potência, rompendo com estereótipos negativos predominantes nas demais linguagens

Film language is an important resource for health teaching-learning. Discussions on cancer and its illness process have used several movies to trigger reflections necessary for a more empathic, sensitive and humanized health care performance. Seeking to contribute to this scenario, this study investigates the meanings around cancer mobilized by the movie Aquarius. The narrative portrays cancer as an invasion against the healthy body and as theft of one's life. Sexuality and female empowerment are intertwined in this film that metaphorizes cancer in its multiple social representations, opening up space for a sense of power and breaking with negative stereotypes prevalent in other languages

Le langage cinématographique est une ressource importante pour l'enseignement et l'apprentissage dans le domaine de la santé. Les discussions sur le cancer et sont processus de maladie ont utilisés plusieurs films pour déclencher les réflexions nécessaires à une performance professionnelle plus empathique, sensible et humanisée. Cherchant à contribuer à ce scénario, cette étude examine les significations au tour du cancer mobilisées par le film Aquarius. Le récit dépeint le cancer comme une invasion du corps sain et comme le vol de la vie. La sexualité et le protagonisme féminin sont entrelacés dans ce film qui métaphorise le cancer dans ses multiples représentations sociales, ouvrant un espace pour un sentiment de pouvoir et rompant avec les stéréotypes négatifs prévalant dans d'autres langues

El lenguaje cinematográfico es un recurso importante para la enseñanza-aprendizaje en el campo de la salud. Para trabajar sobre el tema del cáncer, se han utilizado de películas como desencadenantes de reflexiones necesarias para la formación de profesionales de la salud capaces de un desempeño más sensible y humanizado. Para contribuir a este escenario, el presente estudio tuvo como objetivo discutir los significados sobre el cáncer con base en el análisis de la película Aquarius. Los significados predominantes en esta película se refieren al cáncer como una invasión del cuerpo y como el robo de algo importante para el sujeto. Los temas de la sexualidad y el protagonismo femenino se entrelazan en la creación de una película que metaforiza el cáncer en sus múltiples representaciones sociales, abriendo espacio para la sensación de poder, rompiendo con los estereotipos negativos que prevalecen en otros idiomas

Vaccination impact: mortality and time shift to Covid-19 maximum severity in hospitalized patients - An Argentine multicenter registry / Mortalidad y tiempo entre el inicio y máxima gravedad de COVID-19, en pacientes hospitalizados vacunados y no vacunados. Registro multicéntrico argentino

Abstract Introduction : The COVID-19 vaccine became an effec tive instrument to prevent severe SARS-CoV-2 infections. However, 5% of vaccinated patients will have moderate or severe disease. Objective: to compare mortality and days between the symptom onset to the peak disease severity, in vaccinated vs. unvaccinated COVID-19 hos pitalized patients. Methods : Retrospective observational study in 36 hospitals in Argentina. COVID-19 adults admitted to general wards between January 1, 2021, and May 31, 2022 were included. Days between symptoms onset to peak of severity were compared between vaccinated vs. unvaccinated patients with Cox regression, adjusted by Propensity Score Matching (PSM). Results in patients with one and two doses were also compared. Results : A total of 3663 patients were included (3001 [81.9%] unvaccinated and 662 [18%] vaccinated). Time from symptom onset to peak severity was 7 days (IQR 4-12) vs. 7 days (IQR 4-11) in unvaccinated and vacci nated. In crude Cox regression analysis and matched population, no significant differences were observed. Regarding mortality, a Risk Ratio (RR) of 1.51 (IC95% 1.29-1.77) was observed in vaccinated patients, but in the PSM cohort, the RR was 0.73 (IC95% 0.60-0.88). RR in patients with one COVID-19 vaccine dose in PSM adjusted population was 0.7 (IC95% 0.45-1.03), and with two doses 0.6 (IC95% 0.46-0.79). Discussion : The time elapsed between the onset of COVID-19 symptoms to the highest severity was simi lar in vaccinated and unvaccinated patients. However, hospitalized vaccinated patients had a lower risk of mortality than unvaccinated patients.

Resumen Introducción : A pesar de la eficacia de la vacuna contra el COVID-19 el 5% de los pacientes vacunados presentaran una enfermedad moderada o grave. El ob jetivo del presente estudio fue comparar los días entre el inicio de los síntomas y la gravedad máxima de la enfermedad, en pacientes con COVID-19 vacunados vs. no vacunados. Métodos : Estudio observacional retrospectivo en 36 hospitales de Argentina. Se incluyeron adultos con CO VID-19 hospitalizados entre el 1/01/2021 y 31/5/2022. Se recolectaron datos demográficos, comorbilidades y progresión clínica de la enfermedad. Se compararon los días entre el inicio de los síntomas y el pico de gravedad entre vacunados y no vacunados mediante regresión de Cox, ajustada por emparejamiento por Propensity Score Matching (PSM). En un análisis de subgrupos, se compararon los resultados en pacientes con una y dos dosis de vacuna. Resultados : Se incluyeron 3663 pacientes (3001 [81.9%] no vacunados y 662 [18%] vacunados). El tiempo transcurrido desde el inicio de los síntomas hasta el pico de gravedad fue de 7 días (IQR 4 - 12) en no vacunados, y de 7 días (IQR 4-11) en vacunados. Tanto en el análisis de regresión de Cox crudo como en el ajustado, no se observaron diferencias significativas entre ambos grupos (HR ajustado 1.08 [IC 95% 0.82-1.4; p = 0.56]). En cuanto a la mortalidad, el Riesgo Relativo (RR) fue 1.51 (IC95% 1.29-1.77) en los pacientes vacunados, pero en la cohorte ajustada por Propensity Score, el RR fue de 0.73 (IC95% 0.60-0.88). El RR en el grupo con una dosis de vacuna COVID-19 en el análisis PSM fue 0.7 (IC95% 0.45-1.03), y con dos dosis 0.6 (IC95% 0.46-0.79). Discusión : El tiempo entre el inicio de los síntomas de COVID-19 y el pico de severidad fue igual en vacu nados y no vacunados. Sin embargo, los pacientes va cunados hospitalizados presentaron menor mortalidad tras el ajuste por confundidores.

Evaluation de la performance de la surveillance intégrée de la maladie et de la riposte dans la zone sanitaire Ouidah-Kpomassè-Tori-Bossito, au Bénin / NA

Introduction : La Surveillance Intégrée des Maladies et Riposte est une stratégie initiée en 1998 par le Bureau Régional de l'Organisation mondiale de la Santé pour l'Afrique, pour renforcer la capacité des pays africains à mener des activités de surveillance des maladies transmissibles et réagir efficacement face aux situations d'urgence sanitaire. L'objectif de la présente étude était d'évaluer la performance de la surveillance intégrée des maladies et riposte dans la zone sanitaire Ouidah-Kpomassè-Tori-Bossito en 2020. Méthodes : Il s'agissait d'une étude évaluative, ayant porté sur les formations sanitaires et le personnel impliqué dans la surveillance épidémiologique, sélectionnés respectivement par choix aléatoire simple et par choix raisonné. La performance de la surveillance intégrée des maladies et riposte a été appréciée par les éléments constitutifs des composantes "structure", "processus", "résultats" conformément au modèle de Donabedian et selon l'échelle de Varkevisser. Résultats: Au total 19 formations sanitaires et 19 agents de santé ont été inclus dans l'étude. La performance de la surveillance intégrée des maladies et riposte était moyenne, avec un bon niveau de la composante "structure" et un niveau moyen pour les composantes "processus" et "résultats". D'importantes insuffisances avaient été observées sur les fonctions confirmation, analyse, rétro-information et complétude des rapports. Conclusion: La performance de la surveillance intégrée des maladies et riposte nécessite une amélioration en vue d'une riposte appropriée face aux urgences de santé publique dans la zone sanitaire et une résilience du système de santé. Mots-clés: Performance, surveillance intégrée des maladies et riposte

Introduction: Integrated Disease Surveillance and Response is a strategy initiated in 1998 by the World Health Organization Regional Office for Africa, to strengthen the capacity of African countries to conduct communicable disease surveillance and response activities. effectively in health emergencies. The objective of this study was to assess the performance of integrated disease surveillance and response in the Ouidah-Kpomassè-Tori-Bossito health zone in 2020. Methods: This was an evaluative study, having focused on health facilities and personnel involved in epidemiological surveillance, selected respectively by simple random choice and by reasoned choice. The performance of the integrated disease surveillance and response was assessed by the constituent elements of the components "structure", "process", "results" in accordance with the Donabedian model and according to the Varkevisser scale. Results: A total of 19 health facilities and 19 health workers were included in the study. The performance of integrated disease surveillance and response was average, with a good level of the "structure" component and an average level for the "process" and "outcome" components. Significant shortcomings were observed in the confirmation, analysis, feedback and completeness of reports functions. Conclusion: The performance of integrated disease surveillance and response requires improvement for an appropriate response to public health emergencies in the health zone and health system resilience

Facteurs associés à la survenue du diabète chez les patients admis dans les services d' accueil des urgences de deux hôpitaux de Dakar en 2018 (Sénégal): Étude transversale / NA

Introduction :Au Sénégal, le profil épidémiologique en termes de diabète n'est pas clairement établi chez les patients reçus dans les services d'accueil des urgences (SAUs). L'objectif était de déterminer les facteurs associés à la survenue du diabète chez les patients admis dans les SAUs de deux hôpitaux de Dakar.Méthodes : L'étude était transversale et analytique. La collecte des données a eu lieu du 25 janvier au 05 mars 2018. La taille de l'échantillon était de 615 patients. L'entretien en face en face était réalisé. Les facteurs de risque étaient identifiés à l'aide d'une régression logistique. Les résultats étaient exprimés à l' aide de l'odds ratio ajusté (ORa) et entouré de son intervalle de confiance à 95% (IC95%) Résultats : Les patients étaient des hommes, âgés de moins de 40 ans, mariés et sans emploi dans respectivement 52,4%, 44,5%, 64,5% et 53,3% des cas. En outre, la consommation des fruits et légumes et la pratique du sport étaient insuffisantes chez respectivement 96,4% et 72% des patients. Par ailleurs, 16,9% des sujets interrogés étaient diabétiques. Les facteurs de risque de la survenue de la maladie étaient l'âge avancé, le statut sans emploi et l'obésité: patients âgés entre 40 et 69 ans (ORa=21,184; IC95%=[6,11-73,41]), patients âgés d'au moins 70 ans (ORa=12,62; IC95%=[3,29-48,28]), patients sans travail (ORa=3,47; IC95%=[1,69-7,10]) et patients obèses (ORa=3,17; IC95%=[1,35-7,45]).Conclusion : La fréquence du diabète est élevée chez les patients admis en consultation dans les SAUs des hôpitaux de Dakar. Cette étude montre que recherche du diabète chez cette catégorie de patients devrait être une pratique courante et qu'il urge de mettre en place des actions de promotion de la santé.

Descompresión neurovascular en neuralgia trigeminal: resultado funcional / Neurovascular decompression in trigeminal neuralgia: Functional outcome

Resumen Introducción : La afección más frecuente del V nervio craneal es la neuralgia del trigémino (NT), se caracteriza por dolor orofacial unilateral, paroxístico, distribuyén dose en una o más divisiones del nervio trigémino. El objetivo de presente trabajo es demostrar la eficacia y seguridad de la cirugía descompresiva neurovascular (DNV). Métodos : Estudio analítico retrospectivo, pacientes intervenidos para DNV por NT (n: 155), desde enero de 2006 - 2022, mediante un abordaje retrosigmoideo y seguimiento clínico-radiológico, cuyo registro intraope ratorio fue en sistema 3D (n: 42). Resultados : 83.3% (n=35) presentó NT clásica y 16.7% (n = 7) idiopática. La mayoría con afectación del lado derecho 59.5% (n = 25) y el dolor paroxístico con 81% (n = 34). La compresión de origen arterial representó 76.2% (n: 32) de los casos, la arteria cerebelosa superior representó la primera causa de compresión neurovas cular (CNV) en un 52.4% (n:22) en la zona de salida del nervio trigémino. Se analizó la validez del protocolo de resonancia magnética (RM) seleccionado para esta serie; evidenciando una alta sensibilidad de la RM con 97%, y una especificidad del 86%. Conclusión : Se demostró la sensibilidad y especi ficidad de los estudios de resonancia magnética (RM) de alta definición y con protocolos especiales para la evaluación de la NT, como paraclínico estándar de oro. La DNV ofreció resultados seguros, un alto porcentaje de los pacientes se calificaron como exitosos. El registro 3D permitió analizar en retrospectiva con una visión idéntica a la del cirujano durante el intraoperatorio, el tipo de CNV.

Abstract Introduction : The most frequent pathology of the fifth cranial nerve is trigeminal neuralgia (TN), characterized by unilateral orofacial pain, of a paroxysmal nature, with distribution in one or more divisions of the trigeminal nerve. The main objective of this work is to demonstrate the efficacy and safety of neurovascular decompressive surgery (NVD). Methods : Retrospective analytical study, patients operated on for NVD by TN (n: 155), from January 2006 - 2022, using a retrosigmoid approach and clinical-radiological follow-up, whose intraoperative recording was in 3D system (n: 42). Results : 83.3% (n=35) presented classic NT and 16.7% (n = 7) idiopathic. The right side prevailed with 59.5% (n = 25) and paroxysmal pain with 81% (n=34) of rep resentation in the entire series. Compression of arte rial origin represented 76.2% (n: 32) of the cases, the superior cerebellar artery represented the first cause of neurovascular compression (NVC) in 52.4% (n: 22) in the exit zone of the trigeminal nerve. The validity of the magnetic resonance imaging (MRI) protocol selected for this series was analyzed; evidencing a high sensitivity of MRI with 97%, and a specificity of 86%. Conclusion : The sensitivity and specificity of high-definition MRI studies and with special protocols for the evaluation of TN, as gold standard paraclinical, were presented. The DNV offered safe results, a high percent age of the patients were described as successful. The 3D recording allowed retrospective analysis with a vision identical to that of the surgeon during the intraoperative period, the type of NVC.

Os arquivos no centro das questões de identidade, imediatismo e memória / Les archives au cœur des enjeux d'identité, d'immédiateté et de mémoire / Archives at the heart of issues of identity, immediacy, and memory

Os arquivos estão no centro de práticas e políticas contraditórias: por um lado, produção massiva, recolha imediata, exploração instantânea e publicação aberta; por outro lado, esquecimento, destruição ou negligência, e acesso fechado. O artigo ilustra essas contradições comparando, em primeiro lugar, três iniciativas de recolha de arquivos que permitiram e permitem recuperar o lugar do 'invisível' nas nossas sociedades, contra duas operações mais coletivas na sequência da tragédia francesa de 13 de Novembro de 2015 e dos períodos de confinamento. Depois da recolha, colocam-se questões relacionadas com as tensões geradas pela produção digital: como gerir o fluxo contínuo, a exigência de imediatismo, o recuo da presença humana? Finalmente, num contexto em que coexistem políticas públicas de memória e medidas regulamentares destinadas a proteger o segredo de defesa e o direito ao esquecimento, como podem arquivistas e historiadores trabalhar em conjunto para garantir que esta memória possa ser restituída aos cidadãos?

Les archives sont au cœur de pratiques et politiques contradictoires : d'un côté production massive, collecte immédiate, exploitation instantanée et publication ouverte ; de l'autre oubli, destruction ou négligence, fermeture de l'accès. L'article illustre ces contradictions en mettant tout d'abord en regard trois initiatives de collectes d'archives qui ont permis et permettent de restituer la place des « invisibles ¼ dans nos sociétés, face à deux opérations plus collectives par suite du drame français du 13 novembre 2015 et des périodes de confinement. Après la collecte viennent les questions relatives aux tensions générées par la production numérique : comment gérer le flux continu, la demande d'immédiateté, le recul de la présence humaine ? Enfin, dans un contexte où voisinent politiques publiques mémorielles et dispositifs réglementaires destinés à protéger le secret-défense et le droit à l'oubli, comment peuvent travailler archivistes et historiens pour que cette mémoire puisse être restituée aux citoyens ?

Archives are at the heart of contradictory practices and policies: on the one hand, massive production, imme-diate collection, instantaneous exploitation, and open publication; on the other, forgetfulness, destruction or negligence, and closure of access. The article illustrates these contradictions by, first of all, comparing three archive collection initiatives that have allowed and allow us to restore the place of the 'invisible' in our societies in the face of two more collective operations following the French drama of November 13, 2015, and the confinement periods. After the collection come the questions relating to the tensions generated by digital production: how to manage the continuous flow, the demand for immediacy, and the declining human presence? Finally, in a context where public memorial policies and regulatory measures intended to protect defense secrecy and the right to be forgotten coexist, how can archivists and historians work to restore this memory to citizens?

Peste Des Petits Ruminants: An Update

Peste des petits ruminants (PPR) is a notifiable and reportable disease recognised by the Office Internationale Epizooties (OIE) and penned for eradication by 2030. It is an endemic and devastating disease of small ruminants in some countries of Africa, Asia, and the Middle East. The disease militates against small ruminant production and increased flock numbers in the region. Information and literature on the current review was especially obtained and compiled from CABI abstract data base, PubMed, Scopus, Elibrary USA and other online publications. This review highlights the viral structure, epidemiology, predisposing factors, clinical signs, pathology, immunoprophylaxis, diagnostic techniques and therapeutics of the disease with a view to generating increased scientific research interests on PPR. It is hoped that more robust scientific research breakthroughs could emerge particularly in the area of immunoprophylaxis, rapid penside diagnosis, and alternative therapeutics such as the use of probiotics. This will in no small measure boost small ruminant production, alleviate poverty and increase food security in countries endemic with the disease.

Paternidade e configurações vinculares nos transtornos alimentares à luz da psicanálise vincular / Parenthood and linking configurations in eating disorders in the light of linking psychoanalysis / Parentalité et configurations d'attachement dans les troubles alimentaires à la lumière de la psychanalyse des liens / La paternidad y las configuraciones de apego en los trastornos alimentarios a la luz del psicoanálisis del apego

Ao contrário do vínculo mãe-filha(o), a relação pai-filha(o) tem recebido pouca atenção nos Transtornos Alimentares (TAs). Este estudo com desenho clínico-qualitativo objetivou compreender como pais de jovens com TAs vi,0 realizadas entrevistas semidirigidas com cinco pais cujas(os) filhas(os) estavam em tratamento. Os resultados foram analisados a partir da Análise de Conteúdo Clínico-Qualitativa e da Psicanálise das Configurações Vinculares. A relação pai-filha foi marcada por proximidade afetiva na infância, embora com pouco envolvimento com as tarefas de cuidado. O distanciamento afetivo, a partir do período pubertário da(o) filha(o), contribuiu para fragilizar o vínculo paterno. Os resultados fornecem subsídios para a inclusão dos pais no tratamento.

Unlike the mother-daughter bond, the father-daughter relationship has received little attention in eating disorders (ED). This study with clinical-qualitative method aimed to understand how parents of young people with ED experienced the bonds with their parental figures during childhood/adolescence and, later, with their wives and daughters. Semi-structured interviews were conducted with five parents whose daughters were in treatment. The results were analyzed using the Clinical-Qualitative Content Analysis and the Psychoanalysis of Linking Configurations. The father-daughter relationship was marked by affective closeness in childhood, although with little involvement in caregiving tasks. The affective distancing, starting in the daughter's pubertal period, contributed to weaken the paternal bond. The results provide subsidies for the inclusion of fathers in the treatment.

Contrairement au lien mère-fille, la relation père-fille a reçu peu d'attention dans le domaine des troubles alimentaires (TA). Cette étude avec la méthode clinico-qualitative visait à comprendre comment les parents de jeunes atteints de troubles de l'élocution ont vécu les liens avec leurs figures parentales pendant l'enfance / adolescence et, plus tard, avec leurs épouses et leurs filles. Des entretiens semi-structurés ont été menés avec cinq parents dont les filles étaient en traitement. Les résultats ont été analysés sur la base de l'analyse de contenu clinico-quanitative et de la psychanalyse des configurations d'attachement. La relation père-fille était marquée par une proximité affective dans l'enfance, mais avec une faible implication dans les tâches de soins. La distanciation affective, dès la période de puberté de la fille, a contribué à affaiblir le lien paternel.

La relación padre-hija ha recibido poca atención en los trastornos alimentarios (TAs). Este estudio, con un diseño clínico-cualitativo, pretendía comprender cómo los padres de jóvenes con TAs experimentaban los vínculos con sus figuras parentales durante la infancia/adolescencia y, posteriormente, con sus esposas e hijas. Se realizaron entrevistas semiestructuradas a cinco padres cuyas hijas estaban en tratamiento. Los resultados se analizaron a partir del Análisis de Contenido Clínico-Cualitativo y del Psicoanálisis de las Configuraciones de Apego. La relación padre-hija estuvo marcada por la cercanía afectiva en la infancia, aunque con poca implicación en las tareas de cuidado. El distanciamiento afectivo, a partir del período de pubertad de la hija, contribuyó a debilitar el vínculo paterno. Los resultados proporcionan subsidios para la inclusión de los padres en el tratamiento.

Sentidos de (des)regulação do mercado de trabalho no setor da saúde em Portugal / Directions of health labor market (de)regulation in Portugal

Resumo Reconhece-se a escassez de investigação sobre o mercado de trabalho do setor da saúde. Com a ampliação dos fenómenos de precarização objetiva e subjetiva, decorrente da agenda neoliberal de mercadorização do valor do trabalho e das reformas gestionárias da saúde, a "atipicidade" dos vínculos laborais e a "insegurança" de vida dos profissionais de saúde têm introduzido lógicas de segmentação e polarização do mercado de trabalho. Este deixa de ser palco apenas de mercados protegidos, passando a incluir mercados desregulados e "híbridos". Neste artigo pretende-se explorar os sentidos, com implicações desiguais, da (des)regulação do mercado de trabalho no setor da saúde em Portugal. Parte-se de fontes secundárias e de investigação qualitativa suportada em 32 entrevistas semiestruturadas feitas com profissionais de saúde, que atestam o fenômeno da crescente individualização e subcontratação laboral. Propõe-se um modelo conceitual que capte os sentidos de (des)regulação, num continuum de regulação social e mobilidade profissional, traduzindo mercados protegidos (profissionais e internos) e mercados híbridos (terciarizados e secundários) a serem testados por confronto empírico e investigação futura.

Abstract The need for further research on the labor market in the health sector is acknowledged. With the expansion of the phenomena of objective and subjective precariousness, resulting from the neoliberal agenda of commodification of the value of work and the managerial reforms in health, the "atypicality" of labor ties and the "insecurity" of health professionals' lives have introduced segmentation and polarization logics into the labor market. The latter is no longer the stage for protected markets, but includes deregulated and "hybrid" markets. This article aims to explore the directions, with unequal implications, of the (de)regulation of the labor market in the health sector in Portugal. We begin from secondary and qualitative research supported by 32 semi-structured interviews with health professionals, which attest to the phenomenon of increasing individualization and labor subcontracting. A conceptual model is proposed that captures the meanings of (de)regulation, in a continuum of social regulation and professional mobility, translating protected markets (professional and internal) and hybrid markets (outsourced and secondary) to be tested by empirical confrontation and future research.

Research progress of interaction between peste des petits ruminants virus and host cells / 中国生物制品学杂志

@#Peste des petits ruminants(PPR)is a highly contagious disease with high mortality which is caused by peste des petits ruminants virus(PPRV)infection and leads to enormous economic losses for sheep husbandry both at home and abroad. The knowledge of interactions between PPRV and host cells is the basis of understanding of the pathogenesis and the prerequisite of disease prevention and control. This paper reviews the studies on the activation of host innate immune response by PPRV infection,the immune evasion of PPRV mediated by viral protein products,and the induction of apoptosis,autophagy and inflammasome resistance to virus,which provides a reference for further discussion on the immune evasion of PPRV and the key nodes involved in the interaction between PPRV and the host,thus providing theoretical guidance for effective prevention and control of PPR transmission.

Progress in research on detection techniques for peste des petits ruminants virus / 中国生物制品学杂志

@#Peste des petits ruminants(PPR)is an acute and highly contagious disease caused by peste des petits ruminants virus(PPRV),which mainly infects goats and sheep with high morbidity and mortality. Because of its serious pathological damage and wide spread,PPR has caused huge economic losses to the aquaculture industry and international trade in various countries,so it is particularly important to establish a rapid and accurate detection method for the prevention and control of the disease. This paper reviews the progress in research on biological detection techniques for PPRV detection,such as routine RT-PCR,routine multiplex PCR,real-time fluorence quantitative PCR(RT-qPCR),pyrosequencing,nested PCR(nPCR),loop-mediated isothermal amplification(LAMP)and recombinase polymerase amplification(RPA),so as to provide bases and ideas for scientific detection and identification of PPRV.

Preparation of colloidal gold test strips for the detection of antibodies to peste des petits ruminants based on monoclonal antibodies to N protein / 生物工程学报

A simple, fast, and visual method for detecting antibodies against peste des petits ruminants virus (PPRV) using colloidal gold strips was developed. In this study, the pET-32a-N was transformed into Escherichia coli Rosetta (DE3) for expression. Hybridoma cell lines were generated by fusing SP2/0 myeloma cells with splenocytes from immunized mice with the expressed and purified N protein of PPRV. The PPRV N protein was labeled with colloidal gold particles as the gold-labeled antigen. The N protein served as the gold standard antigen and as the test (T) line-coated antigen, while the monoclonal antibody served as the quality control (C) line-coated antibody to assemble the colloidal gold immunochromatographic test strips for detecting antibodies against the N protein of PPRV. Hybridoma cell line designated as 1F1 was able to stably secrete the monoclonal antibody against the N protein of PPRV. The titer of 1F1 monoclonal antibody in ascites was 1:128 000 determined by indirect enzyme-linked immunosorbent assays (ELISA), and the immunoglobulin subtype of the monoclonal antibody was IgG1, with kappa chain. The obtained monoclonal antibody was able to specifically recognize the N protein of PPRV, as shown by Western blotting and indirect immunofluorescent assay (IFA). The developed colloidal gold test strip method was able to detect PPRV antibodies specifically, and there was no difference between different batches of the test strips. Testing of a total of 122 clinical sera showed that the compliance rate of the test strip with ELISA test was 97.6%.The test strip assay developed in this study has good specificity, reproducibility, and sensitivity, and it can be used for the rapid detection of PPRV antibodies.

Saúde mental e o impacto da pandemia de covid-19 nos estudantes de uma universidade pública de Manaus-Am / Mental health and the impact of the covid-19 pandemic on students of a public university of Manaus-Am / Salud mental y el impacto de la pandemia covid-19 en los es- tudiantes de una universidad pública de Manaus-Am

Objetivo: Descrever as repercussões da Covid-19 na saúde mental dos estudantes de enfermagem de uma instituição de ensino superior no estado do Amazonas, Brasil. Metodologia: Trata-se de uma pesquisa quantitativa e transversal realizada com 92 estudantes da Escola de Enfermagem de Manaus, da Universidade Federal do Amazonas (EEM/UFAM) matriculados no curso de enfermagem durante período pandêmico. Os dados foram coletados a partir de um questionário composto por três partes: Perfil sociodemográfico; Questões sobre condições de aprendizagem e isolamento social em relação à pandemia adaptado de Oliveira et al. (2022); e Escala de Sintomas de Estresse validado por Lima, Formiga e Melo (2018). Sendo, os dados analisados por meio de Análise Estatística Descritiva. Resultados: O perfil sociodemográfico dos estudantes de Enfermagem traçado foi de mulheres em geral (89,1%), que 53,3% se autodeclaram como pardos, solteiros (90,2%), oriundos de Manaus - AM (87%), moradia própria (70,7%), de 3 a 5 moradores (60,9%) e o carro sendo o principal meio de transporte com 54,3%. Os sintomas de estresse durante a pandemia de Covid-19 traçaram que 41,3% dos estudantes têm pensamentos que provocam ansiedade, cansaço (55,4%), desânimo (43,5%), esgotados emocionalmente (41,3%), exaustão física (47,8%), preocupações (42,4%) e vontade de ficar sozinho (42,4%). Conclusão: Espera-se, portanto, que esse estudo propicie qualificar o cuidado aos estudantes universitários, no qual se possa realizar ações preventivas para os impactos na saúde mental e ainda, contribua a futuras pesquisas que investiguem outras realidades estudantis tanto em meio público como privado.

Objective: To describe the repercussions of COVID-19 on the mental health of nursing students at a higher education institution in the state of Amazonas, Brazil. Methodology: It is a quantitative and cross-sectional research carried out with 92 stu- dents from the Manaus Nursing School, Federal University of Amazonas (EEM/UFAM) enrolled in the nursing course during pandemic period. Data were collected from a questionnaire composed of three parts: Sociodemographic profile; Questions about learning conditions and social isolation in relation to the pandemic adapted de Oliveira et al. (2022); and Stress Symptom Scale validated by Lima, Ant and Melo (2018). Thus, the data analyzed by means of Descriptive Statistical Analysis. Results: The sociodemographic profile of the Nursing students was drawn up of women in general (89.1%), that 53.3% self-declared as mulatto, single (90.2%), coming from Manaus - AM (87%), own housing (70.7%), from 3 to 5 residents (60.9%) and the car being the main means of transport with 54.3%. Symptoms of stress during the COVID-19 pandemic have traced that 41.3% of students have thoughts that provoke anxiety, tiredness (55.4%), discouragement (43.5%), emotionally exhausted (41.3%), physical exhaustion (47.8%), worries (42.4%), and willingness to be alone (42.4%). Conclusion: It is hoped, therefore, that this study will make it possible to qualify the care of university students, in which preventive actions can be carried out for the impacts on mental health, and also, contribute to future research that investigates other student realities both in public and private environments.

Propósito: Describir las repercusiones de Covid-19 en la salud mental de los estudiantes de enfermería de una institución de educación superior en el estado de Amazonas, Brasil. Metodología: Se trata de una encuesta cuantitativa e intersectorial re- alizada con 92 estudiantes de la Escuela de Enfermería de Manaus, de la Universidad Federal del Amazonas (EEM/UFAM) inscritos en el curso de enfermería durante un pe- ríodo de pandemia. Los datos se recogieron a partir de un cuestionario compuesto por tres partes: perfil sociodemográfico; preguntas sobre las condiciones de aprendizaje y el ais- lamiento social en relación con la pandemia adaptada de Oliveira y otros (2022); y una escala de síntomas de estrés validada por Lima, Ant y Melo (2018). Los datos se analizan mediante análisis estadístico descriptivo. Resultados: El perfil sociodemográfico de los estudiantes de enfermería se obtuvo de las mujeres en general (89,1%), que 53,3% se declaran marrones, solteros (90,2%), provenientes de Manaus - AM (87%), de su propia vivienda (70,7%), de 3 a 5 habitantes (60,9%) y de su automóvil como principal medio de transporte. con 54,3%. Los síntomas de estrés durante la pandemia de Covid-19 han mostrado que el 41,3% de los estudiantes tienen pensamientos que causan ansiedad, can- sancio (55,4%), desánimo (43,5%), agotamiento emocional (41,3%), agotamiento físico (47,8%), preocupaciones (42,4%) y deseo de estar solos (44) 2,4%). Conclusión: Por lo tanto, se espera que este estudio proporcione una calificación para la atención de los es- tudiantes universitarios, en la que se puedan llevar a cabo acciones preventivas para el impacto en la salud mental y, aun así, contribuya a futuras investigaciones que investi- guen otras realidades estudiantiles tanto en los círculos públicos como en los privados.

Impact of drug-eluting stent-associated coronary artery spasm on 3-year clinical outcomes: A propensity score matching analysis

Background: It has been reported that significant endothelial dysfunction or clinically evident vasospasm can be associated with drug-eluting stents (DESs). However, the impact of DES associated coronary artery spasm (CAS) on long-term clinical outcomes has not been fully elucidated as compared with those of patients with vasospastic angina. Methods: A total of 2797 consecutive patients without significant coronary artery lesion (<70%), who underwent the Acetylcholine (Ach) provocation test, were enrolled between Nov 2004 and Oct 2010. DES-associated spasm was defined as significant CAS in proximal or distal to previously implanted DES site at follow-up angiography with Ach test. Patients were divided into two groups (DES-CAS; n ¼ 108, CAS; n ¼ 1878). For adjustment, propensity score matching (PSM) was done (C-statistics ¼ 0.766, DESCAS; n ¼ 102, CAS; n ¼ 102). SPSS 20 (Inc., Chicago, Illinois) was used to analyze this data. Results: Baseline characteristics were worse in the DES-CAS group. After PSM, both baseline characteristics and the Ach test results were balanced except higher incidence of diffuse CAS and ECG change in the DES-CAS group. During Ach test, the incidence of diffuse spasm (93.1% vs. 81.3%, p ¼ 0.012) and ST-T change (10.7% vs. 1.9%, p ¼ 0.010) were higher in the DES-CAS group. At 3-year, before and after adjustment, the DES-CAS group showed a higher incidence of coronary revascularization (9.8% vs. 0.0%, p ¼ 0.001), recurrent chest pain requiring follow up coronary angiography (CAG, 24.5% vs. 7.8%, p ¼ 0.001) and major adverse cardiac events (MACEs, 9.8% vs. 0.9%, p < 0.005). Conclusion: In this study, DES associated CAS was associated with higher incidence of diffuse spasm, ST-T change and adverse 3-year clinical outcomes. Special caution should be exercised in this particular subset of patients.

Drug Eluting Stents versus Coronary Artery Bypass Graft Surgery for Patients with Diabetes Mellitus and Multivessel Disease

Background: through coronary artery bypass graft (CABG) or through utilization of percutaneous coronary intervention (PCI) with stenting, coronary revascularization can be achieved. Diabetics represents a particularly challenging group for both treatments. This work aimed to estimate clinical outcome in diabetic individuals with multiple vessels disease (MVD) who had either PCI or CABG over 30 days. Methods: This prospective research was conducted on 200 diabetic individuals with MVD established as severe stenosis. Into 2 equal groups, individuals were divided: Group (A) [underwent CABG], and group (B) [performed Drug Eluting Stents (DES) PCI]. All individuals underwent full history taking, resting twelve-leads electrocardiogram, complete clinical examination, transthoracic echocardiography, coronary angiographic, SYNTAX score and Euro score. Results: In CABG group, age, statin, ACE-I/ARB, ?- -blocker and dual antiplatelet therapy was significantly higher than PCI group. PCI group had significantly higher PCI, MI and repeated revascularization than the group of CABG. In PCI group, stroke and MI, death, myocardial infarction (MI), were significantly higher than in group of CABGS. Conclusions: DES have developed for the coronary artery disease (CAD) treatment and are increasingly being utilized for complex CAD treatment, such as multivessel or left main CAD. PCI is preferred over CABG in high surgical risk individuals due to the shorter hospital stay, faster time of recovery, and potentially decreased stroke rate

Educação Física (des)seriada e a oportunidade de equidade na motivação de meninos e meninas / (Un)serious Physical Education and the opportunity for equity in the motivation of boys and girls / La (des)seriación de la Educación Física y la oportunidad de equidad en la motivación de niños y niñas

Este estudo objetivou identificar a motivação de meninos e meninas às aulas de Educação Física do ensino médio, considerando a proposta diferenciada de organização das turmas, denominada (Des)seriação. Analisou-se, por meio dos testes estatísticos descritivos e de comparação de grupos (Mann-Whitney), 1071 instrumentos preenchidos de 2012 a 2018. Observou-se que, na maioria dos anos pesquisados, os valores atribuídos às variáveis Motivação Intrínseca e as dimensões das Necessidades Psicológicas Básicas não apresentaram diferença estatisticamente significativa entre os sexos. Concluiu-se que a proposta (des)seriada na Educação Física do ensino médio pode ser uma potente ferramenta de motivação para incentivar a equidade de oportunidades nesse ambiente.

This study aimed to identify the motivation of boys and girls to Physical Education classes in high school, considering the differentiated proposal of class organization, called (Un)seriation. We analyzed, through descriptive statistical tests and comparison of groups (Mann-Whitney), 1071 instruments completed from 2012 to 2018. It was observed that, in most years surveyed, the values attributed to the variables Intrinsic Motivation and the dimensions of Basic Psychological Needs did not show a statistically significant difference between the sexes. It was concluded that the (un)seriated proposal in high school Physical Education can be a powerful motivational tool to encourage equal opportunities in this environment.

El objetivo de este estudio fue identificar la motivación de niños y niñas para las clases de Educación Física en la enseñanza media, considerando la propuesta diferenciada para la organización de las clases, denominada (Des)seriación. Mediante pruebas estadísticas descriptivas y comparación de grupos (Mann-Whitney) se analizaron 1071 instrumentos completados entre 2012 y 2018. Se observó que, en la mayoría de los años encuestados, los valores asignados a las variables Motivación Intrínseca y las dimensiones de Necesidades Psicológicas Básicas no mostró una diferencia estadísticamente significativa entre los sexos. Se concluyó que la propuesta (no)seriada en Educación Física en la enseñanza media puede ser una poderosa herramienta de motivación para incentivar la igualdad de oportunidades en este medio.

Familiares como pesquisadores de serviços de atenção psicossocial: um desafio para a promoção do cuidado e autonomia na perspectiva do recovery / Family members as researchers of psychosocial care services: Challenges for the promotion of care and autonomy in recovery / Les membres de la famille en tant que chercheurs des services de soins psychosociaux: un défi pour la promotion des soins de santé et de l'autonomie dans la perspective du recovery / Los familiares como investigadores de los servicios de atención psicosocial: un desafío para la promoción del cuidado y la autonomía en la perspectiva de recuperación

O presente estudo discute a inserção de familiares cuidadores de pessoas com transtornos mentais graves e persistentes em uma pesquisa acadêmica de modelo participativo. O estudo se apoia no conceito de recovery para discutir a participação ativa de familiares no processo de avaliação dos serviços de saúde mental e de produção compartilhada de conhecimento. Trata-se de uma pesquisa qualitativa que emprega instrumentos de avaliação participativa nas diferentes etapas de inserção de familiares como pesquisadores. Conclui-se sobre o protagonismo de familiares no desenvolvimento de pesquisas como uma ferramenta potente para o recovery na medida em que amplia o conhecimento, qualifica a relação dos usuários com os serviços, fortalece os vínculos sociais e o compartilhamento de experiências e favorece processos emancipatórios e democráticos de controle social.

This qualitative study discusses the inclusion of family caregivers of people with severe and persistent mental disorders in a participatory academic research. Based on the concept of recovery, the paper addresses the active participation of family members in the evaluation process of mental health services and shared knowledge production. Data was collected by means of participatory assessment instruments in the different stages of inserting family members as researchers. In conclusion, the protagonism of family members in research development is a powerful tool for recovery as it expands knowledge, qualifies user relationship with services, strengthens social bonds and the sharing of experiences, and favors emancipatory and democratic processes of social control.

Cette étude qualitative traite de l'inclusion des aidants de personnes atteintes de troubles mentaux sévères et persistants dans une recherche universitaire participative. Basé sur le concept de rétablissement, l'article aborde la participation active des aidants dans le processus d'évaluation des services de santé mentale et la production de connaissances partagées. Les données ont été collectées au moyen d'instruments d'évaluation participative dans les différentes étapes de l'insertion des membres de la famille en tant que chercheurs. En conclusion, le protagonisme des membres de la famille dans le développement de la recherche est un outil puissant pour le rétablissement car il élargit les connaissances, qualifie la relation de l'utilisateur avec les services, renforce les liens sociaux et le partage d'expériences, et favorise les processus émancipateurs et démocratique de contrôle social.

El presente estudio discute la participación de los cuidadores familiares de personas con trastornos mentales graves y persistentes en una investigación académica de modelo participativo. El estudio utiliza el concepto de recuperación para discutir la participación activa de los familiares en el proceso de evaluación de los servicios de salud mental y producción de conocimiento compartido. Esta es una investigación cualitativa que utiliza instrumentos de evaluación participativa en las diferentes etapas de inserción de los familiares como investigadores. Se concluye que el protagonismo de los familiares en el desarrollo de la investigación es una poderosa herramienta de recuperación, ya que amplía el conocimiento, califica la relación de los usuarios con los servicios, fortalece los vínculos sociales y el intercambio de experiencias, y favorece los procesos emancipatorios y democráticos de control social.

Dar lugar à palavra: reverberações da clínica com imigrantes bolivianos num CAPS infantojuvenil / Dando paso a la palabra: reverberaciones de la clínica con inmigrantes bolivianos en un CAPS / Give place to the word: reverberations of the clinic with bolivian immigrants at a CAPS / Faire place aux mots: réverbérations de la clinique avec des immigrants boliviens dans un CAPS

A chegada de crianças filhas de imigrantes bolivianos no CAPS infantojuvenil Mooca vem ocorrendo com maior frequência. Essas crianças chegam ao serviço com queixas de problemas do desenvolvimento, destacando-se atraso na fala e dificuldade na interação, muitos com a hipótese diagnóstica de autismo interrogada. Neste artigo, apresentaremos um grupo terapêutico com imigrantes, que possibilitou um espaço de acolhimento para crianças e famílias e a abertura para narrar e elaborar sobre vivências de sofrimento, desenraizamento e solidão. Buscaremos ampliar as leituras possíveis para os problemas do desenvolvimento apresentados pelas crianças, interrogando como podem estar relacionados à experiência de imigração e seus efeitos sociopolíticos na subjetividade. Por fim, discutiremos como a demanda social por diagnósticos responde à razão psiquiátrica contemporânea e não à demanda de cuidado e escuta das crianças

La llegada de hijos de inmigrantes bolivianos a los CAPS para niños y jóvenes de Mooca viene ocurriendo cada con mayor frecuencia. Estos niños llegan al servicio con retraso en el habla y el desarrollo y dificultad en la interacción, muchos ya con la hipótesis diagnóstica de autismo. Aquí presentaremos un grupo terapéutico con inmigrantes, con un espacio de acogida para niños y familias y la apertura para narrar y elaborar experiencias de sufrimiento, desarraigo y soledad. Buscaremos ampliar las posibles lecturas de los retrasos presentados, cuestionando cómo pueden estar relacionados con la experiencia de la inmigración y sus efectos sociopolíticos en la subjetividad. Finalmente, discutiremos cómo la demanda social de diagnósticos responde a la psiquiatría y no a la demanda de cuidado y escucha de los niños

The arrival of children of Bolivian immigrants at CAPS juvenile Mooca (Psychological Support Center) with speech and developmental delays, has severely increased over time. These children arrive at the center with speech delays, isolation, impairments in social cognition and behavioral, many of them hypothetically diagnosed with autism. In this article, we'll introduce a specific therapeutic group of immigrants that collaborated to describe experiences of suffering, cultural uprooting and loneliness. We will try to broaden the possible reasons for delay in development demonstrated by children, interrogating how it could be related to the immigration experience and its subjective social-political effects. Lastly, we will discuss how the social demand for diagnoses respond to psychiatric reasons as opposed to careand listening to children's necessities

L'arrivée d'enfants fils d'immigrants boliviens chez le CAPS des enfants et des jeunes Mooca est plus fréquente. Ces enfants viennent avec retard dans la parole et dans le développement, des difficultés d'interaction, plusieurs parmi eux avec l'hypothèse diagnostique de autisme. Ici, nous présentons un groupe thérapeutique avec des immigrants, avec un espace d'accueil aux enfants et aux familles, ainsi que l'ouverture pour raconteur et élaborer les vécus de survie et de souffrance, de déracinement et de solitude. Nous chercherons à élargir les lectures possibles des retards présentés par les enfants, en étudiant la relation avec l'expérience d'immigration et ses effets sociopolitiques sur la subjectivité. Nous discutons, de quelle façon la demande sociale les diagnostiques répond à la psychiatrie et non à la demande de soins et d'écoute des enfants

Crianças diagnosticadas em função de impasses na alfabetização: contribuições da psicanálise / Enfants diagnostiqués en fonction des impasses d'alphabétisation: apports de la psychanalyse / Children diagnosed due to literacy problems: contributions of psychoanalysis

O presente artigo descreve os resultados de uma pesquisa/intervenção de orientação psicanalítica por meio do relato de um estudo de caso de um aluno do ensino fundamental de uma escola pública de ensino da cidade de Itabira-MG. O estudante apresentava impasses na alfabetização e entraves na esfera escolar, enfrentados por meio do processo de patologização e medicalização. Contrapondo-se à medicalização, o caso visa demonstrar a contribuição de um dos procedimentos adotados nesta pesquisa: o diagnóstico Clínico-Pedagógico (DCP), cuja intervenção realiza a leitura daquilo que o aluno ensina ao educador sobre sua singularidade e sobre as particularidades que se repercutem na sua relação com o saber e com os laços sociais construídos na escola. Os resultados obtidos por meio do DCP permitiram perceber o impacto do fenômeno da nomeação advinda de um diagnóstico na aprendizagem acadêmica e a possibilidade de utilizar abordagens teóricas e práticas que permitam aos sujeitos agir sobre os seus problemas escolares.

Cet article décrit les résultats d'une recherche/intervention d'orientation psychanalytique à l'aide du rapport d'une étude de cas d'un élève du primaire d'une école publique de la ville d'Itabira-MG. L'étudiant avait des impasses en alphabétisation et des obstacles dans la sphère scolaire, confrontés à travers le processus de pathologisation et de médicalisation. Contrairement à la médicalisation, le cas vise démontrer l'apport d'une des procédures adoptées dans cette recherche: le Diagnostic clinico-pédagogique (DCP), dont l'intervention effectue la lecture de ce que l'élève enseigne à l'éducateur sur sa singularité et sur les particularités qui ont des répercussions sur leur rapport aux savoirs et aux liens sociaux construits à l'école. Les résultats obtenus par le biais du DCP ont permis de percevoir l'impact du phénomène de dénomination issu d'un diagnostic sur les apprentissages scolaires et la possibilité d'utiliser des approches théoriques et pratiques permettant aux sujets d'agir sur leurs problèmes scolaires.

This article describes the results of a psychoanalytically oriented research/intervention through the report of a case study of an elementary school student from a public school in the city of Itabira-MG. The student had impasses in literacy and obstacles in the school life, faced through the process of pathologization and medicalization. In contrast to medicalization, the case aims to demonstrate the contribution of one of the procedures adopted in this research: the Clinical- Pedagogical Diagnosis (DCP), whose intervention performs the evaluation of what the student teaches the educator about his singularity and about the particularities that have repercussions on their relationship with knowledge and with the social bonds built at school. The results obtained through the DCP made it possible to perceive the impact of the naming phenomenon arising from a diagnosis on academic learning and the possibility of using theoretical and practical approaches that allow subjects to act on their school problems.

Outro uso do início do tratamento analítico: as entrevistas preliminares como direção da assistência aos colaboradores / Autre utilisation du début du traitement analytique: les entretiens préliminaires comme direction du service d'assistance aux collaborateurs / Another use of the start of analytical treatment: the preliminary interviews as a direction for the assistance to the employees

Este artigo se fundamenta na articulação entre (1) ferramentas teóricas fornecidas em curso de aperfeiçoamento em psicologia do colaborador e (2) experiências de atuação clínica em um projeto de psicanálise aplicada para assistência aos colaboradores de uma instituição hospitalar. O trabalho é permeado pela questão de como sustentar a prática clínica de orientação psicanalítica na instituição. Neste sentido, argumenta-se que é possível encontrar na psicanálise lacaniana bases para formalizar a experiência de atendimento aos colaboradores. Especificamente, no enquadre do início do tratamento analítico - da sondagem diagnóstica em Freud, e das entrevistas preliminares em Lacan. A articulação tangencia o problema da "entrada" e da "inserção" do psicólogo que estabelece na psicanálise aplicada a efetividade de sua atuação em instituição hospitalar.

Cet article s'appuie sur l'articulation entre (1) des outils théoriques dispensés dans un cours de perfectionnement en psychologie du personnel, et (2) des expériences de pratique clinique dans un projet de psychanalyse appliquée pour assister les employés d'un établissement hospitalier. La question de ce travail est de savoir comment soutenir la pratique clinique d'orientation psychanalytique au sein de cette institution hospitalière. En ce sens, on avance qu'il est possible de trouver, dansla psychanalyse lacanienne, des bases pour formaliser l'expérience d'assistance des collaborateurs. Plus précisément, dans le cadre du début du traitement analytique du sondage diagnostic de Freud, ainsi que dans les entretiens préliminaires de Lacan. L'articulation touche au problème de "l'entrée" et de "l'insertion" du psychologue qui établit dans la psychanalyse appliquée l'efficacité de sa performance dans un établissement hospitalier.

This article is based on the articulation between (1) theoretical tools provided by a course in employee psychology and (2) experiences of clinical practice in an applied psychoanalysis project to assist the employees of a hospital institution. The work is permeated by the question of how to sustain the psychoanalytically oriented clinical practice in the institution. Therefore, it is argued that it is possible to find in Lacanian psychoanalysis bases to formalize the experience of assisting personnel. Specifically, within the frame of the beginning of the analytic treatment - of the diagnostic investigation in Freud, and in the preliminary interviews in Lacan. The study analyses the problem of "entry" and "insertion" of the psychologist who establishes the effectiveness of his performance in applied psychoanalysis at a hospital institution.