Association among Serum Cortisol, Dehydroepiandrosterone-sulfate Levels and Psychiatric Symptoms in Men with Chronic Schizophrenia / 医学研究杂志

Objective The purpose of this study was to examine possible associations of serum levels of cortisol and dehydroepiandrosterone-sulfate (DHEA-S) with psychiatric symptoms in men with chronic schizophrenia.Methods This study involved 120 men with schizophrenia and 110 age-matched healthy controls,and the information of patients were collected on demographic characteristics,age at disease onset,disease duration,positive and negative syndrome scale (PANSS) scores,and history of atypical antipsychotic treatment.Results Serum levels of cortisol and DHEA-S were calculated,as well as the ratios of the two levels.Possible correlations were explored between these levels and psychiatric symptoms before and after antipsychotic treatment.Serum levels of cortisol and DHEA-S levels as well as the ratios of cortisol to DHEA-S levels were more higher in patients than in controls (P ＜ 0.01).Among patients,serum levels of cortisol and DHEA-S were significantly lower after treatment than before (P ＜ 0.01),although the ratios of cortisol to DHEA-S levels remained similar.Serum levels of cortisol,DHEA-S and the ratios of the two levels were positively correlated with the negative symptoms score on the PANSS.Conclusion The pathophysiology of schizophrenia may involve in the spread levels of cortisol and DHEA-S.These levels may serve as biomarkers for diagnosing schizophrenia and monitoring treatment efficacy.

Some hormones changes in women with polycystic ovary syndrome / Монголын Анагаах Ухаан

IntroductionPolycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductive-age women,affecting an estimated 5-8% of all women in this age group. Clinically, hyperandrogenism, chronicanovulation, central obesity and polycystic ovary can all occur in women with PCOS and may causeof infertility. Dehydroepiandrosterone sulfate is an androgenic hormone which produced from adrenalcortex and recently, there is no any data determined DHEA-S in patients with PCOS.GoalOur study was designed to determine serum DHEA-S and other hormones in women with PCOS.Materials and MethodWe have used a cross-sectional study design and the study included reproductive-age 18 women withPCOS. ELISA test to determine serum DHEA-S and other sexual hormones was analyzed for all of thesewomen.ResultWhen we analyzed ELISA test to determine serum DHEA-S and other sexual hormones, DHEA-S level8.0±2.1 μg/ml, LH level 9,45±4,3 mlU/ml, FSH level 5,04±1,1 mlU/ml, prolactin level 19,78±12,2 ng/ml, E2 hormone level 16,8±8,9pg/ml, testosterone level 0,25±0,1 ng/ml were in women with PCOS,respectively. And LH/FSH ratio was 2:1 in these women. (p=0.001).ConclusionBy the result, serum DHEA-S, LH,

Study of Estradiol, Testosterone and DHEA-S Status in Women After Surgical Menopause.

Aims and Objectives : The study was done to assess the hormones namely Estradiol, Testosterone, and Dehydroepiandrosterone-sulphate (DHEAs) one day before operation and on ninth post-operative day following surgical menopause. Materials and Methods : This is a cross sectional observational study. The study was done amongst the thirty four women aged between 40-48 years with functioning uterus and at least one ovary, not using any exogenous hormone preparations affecting ovarian function for last three months and having at least one menstrual period in three previous months were included in this study. They had under gone hysterectomy with bilateral salpingooophorectomy due to non-ovarian pathology. Fasting venous blood samples were taken one day before operation and on ninth post-operative day of surgical menopause Serum concentration of estradiol, testosterone, and DHEAS were determined. Results : The circulating estradiol level decreased significantly (p = 0.043) from 161 pg/ml preoperatively to 108 pg/ml. on ninth post-operative day after surgical menopause. In spite of reduction in mean testosterone level from 0.11 ng/ml. to 0.09 ng/ml. following surgical menopause, which is statistically insignificant (p = 0.247).There was no significant difference between the serum DHEA-S level before and after surgical menopause. A significant positive correlation was observed between pre-operative circulatory levels of DHEA-S with that of estradiol while there was absence of any significant co-relations corelations between any of the other pairs of values. Conclusion : The circulating estradiol level decreased significantly on ninth day after surgical menopause and significant positive correlation between pre-operative circulatory levels of DHEA-S with that of estradiol, but there was no significant co-relation between post-operative circulating estradiol with that of DHEA-S. Testosterone did not show any significant relation with estradiol neither in pre-operative period nor in post-operative condition.

SOME RESULTS FROM SERUM DEHYDROEPIANDROSTERONE SULFATE HORMONE DETECTION IN REPRODUCTIVE-AGED WOMEN WITH PCOS AND HEALTHY / Шинэ санаа Шинэ нээлт

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductive-age women, affecting an estimated 5-8% of all women in this age group. Clinically, hyperandrogenism, chronic amenorrhea, central obesity and polycystic ovary can all occur in women with PCOS and may cause of infertility. Dehydroepiandrosterone sulfate is an androgenic hormone produced inadrenal cortex and recently, there is no any data determined DHEA-S in patients with PCOS.Our study was designed to evaluate obesity, central obesity, hirsute grade and infertility and to determine serum DHEA-S hormone in women with PCOS and healthy non-PCOS women.We have used a cross-sectional study design and the study included reproductive-age 36 women, of whom 18 were diagnosed with PCOS and 18 were non-PCOS. Anthropometric components were measured and ELISA test to determine serum DHEA-S hormone was analyzed for all of these women. When we analyzed ELISA test to determine serum DHEA-S hormone, DHEA-S level was2.9±2.0 µg/ ml in non-PCOS reproductive-age women and 8.0±2.1 µg/ml in women with PCOS, respectively (р=0.01). Among the women diagnosed with PCOS had higher incidence of central obesity and grade II hirsute and infertility than healthy women (p=0.001). By the result, higher level of serum DHEA-S in women diagnosed with PCOS may cause of sign of hyperandrogenism such as central obesity, infertility and hirsute.

Tensión arterial y masa corporal en mujeres infértiles con síndrome de ovario poliquístico y su relación con el perfil hormonal / Blood pressure and body mass in infertile women polycystic ovary syndrome and its relation to the profile hormone

Objetivo: Evaluar perfil hormonal, índice de masa corporal y tensión arterial en 40 mujeres infértiles con ovario poliquístico. Métodos: En la consulta del Centro de Atención Integral de la Universidad de Los Andes se valoró talla, peso y tensión arterial. Durante la fase folicular del ciclo menstrual en ellas se midió en sangre las hormonas sexuales e insulina (basal y 2 horas poscarga glucosada) por electroquimioluminiscencia. Resultados: El índice de masa corporal se correlacionó directamente con el valor de tensión arterial sistólica, la relación LH/FSH y la testosterona sérica; e inversamente con las hormonas FSH y PRL. Los datos clínicos y de laboratorio se observaron dentro de los límites de referencia; sin embargo, la testosterona guardó relación directa con LH/FSH, índice de masa corporal y tensión arterial sistólica. Al compararse dos grupos de paciente con base al valor de la mediana poblacional, los grupos masa corporal > 24 kg/m2 y tensión arterial sistólica >120 mmHg mostraron niveles de DHEA-S e insulina (basal y 2 h) más elevados que en mujeres con índice de masa corporal y tensión arterial sistólica más bajos. Conclusiones: Existe correlación entre niveles séricos de andrógenos con sobrepeso e hipertensión arterial por mecanismos etiológicos interrelacionados. Síndrome de ovario poliquístico es de origen multicausal eventualmente con hiperandrogenemia. La falla metabólica debe controlarse en estas pacientes, lo que permitiría bajar el efecto de los andrógenos y favorecer el estado de fertilidad, pero sobre evita a largo plazo complicaciones como obesidad, diabetes mellitus tipo 2 e hipertensión arterial.

Objective: To evaluate hormonal profile, body mass index and blood pressure in 40 infertile women with polycystic ovary. Methods: In the out patien clinic of the Centro de Atencion Integral de la Universidad de Los Andes, height, weight and blood pressure were assessed. During follicular phase of the menstrual cycle in blood of them sex hormones and insulin (basal, 2 hours post glucose load) were measured by electrochemiluminescence. Results: The body mass index was directly correlated with the value of systolic blood pressure, ratio LH/ FSH and testosterone in serum, and it was inversely correlated with the hormones FSH and PRL. Clinical and laboratory data were observed within the reference limits, but kept directly related to testosterone LH / FSH, BMI and systolic blood pressure. When comparing two patient groups based on the value of the population median, body mass groups > 24 kg/m2 and systolic blood pressure > 120 mmHg, they showed levels of DHEA- S and insulin (basal and 2 h) higher than those women with lower values of BMI and systolic blood pressure. Conclusions: There is a correlation between serum androgen levels with overweight and hypertension by aetiological mechanisms interrelated. Polycystic ovary syndrome is multicausal origin eventually with hyperandrogenemia. Metabolic failure should be monitored in these patients, which would lower the effect of androgens and promote fertility status, but prevents long-term complications such as obesity, type 2 diabetes mellitus and high blood pressure.

Evaluation of performance stress among military submarine soldiers by using salivary cortisol,DHEA-S and cortisol/DHEA-S ratio assay / 军事医学

Objective To investigate the significance of salivary cortisol , dehydroepiandrosterone sulfate (DHEA-S) and cortisol/DHEA-S ratio changes for evaluation of military performance stress .Methods Forty submarine soldiers were selected, whose saliva samples were collected separately at the end of long-term dive training and after nine months of relaxation break.In addition, the saliva samples of thirty-four graduate students were collected the moment they finished a three-hour final examination and one week later .The method of ELISA was used to detect the levels of salivary cortisol and DHEA-S and to count their ratio .Results After long-term dive training , the submarine soldiers showed significantly decreased DHEA-S and an increased cortisol/DHEA-S ratio, but the cortisol level did not change very much .In contrast, the final examination stress did not change the level of cortisol , DHEA-S or the cortisol/DHEA-S ratio among these students.Conclusion This is the first study to show that long-term, chronic military performance stress is associated with the salivary DHEA-S and cortisol/DHEA-S ratio changes .The increase in the cortisol/DHEA-S ratio may be used as an important and useful biomarker to evaluate chronic stress .In addition , it is relatively simple and sensitive to detect stress biomarkers by using saliva samples .

El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 / DHEAS increases levels of GluR2/3 and GluR2, AMPA receptor subunits, in C57BL/6 mice hippocampus

El DHEAS es un neuroesteroide con efecto neuromodulador de la transmisión sináptica y en la neuroprotección, sin embargo las vías moleculares a través de las cuales se inducen estos cambiosno están completamente claras. Como varios de los neuroesteroides actúan a través de los recetores ionotrópicos de glutamato, se evaluó el efecto del DHEAS en las subunidades GluR2 y GluR3 del receptor AMPA para esclarecer sus efectos. Con este fin se administró DHEAS o una sustancia control durante 7 días a ratones C57/BL6. La expresión de las subunidades se evaluó por Westernblotting.Los resultados presentados muestran que la administración prolongada de 40mg/kg/día de DHEAS a ratones C57/BL6 produce un incremento en los niveles de proteína de las subunidades GluR2/3 yGluR2 del receptor AMPA en el hipocampo. Dado el papel específico que juega la subunidad GluR2 del receptor AMPA en el control de la entrada de calcio durante los procesos de muerte celular y de plasticidad sináptica, este hallazgo contribuye al estudio de los neuroesteroides como una estrategia terapéutica relevante en enfermedades neurodegenerativas y eventos cerebrovasculares.

Dehydroepiandrosterone sulfate (DHEA-S) is a neurosteroid that has effects such as neuromodulator of synaptic transmission and neuroprotection. The specific signaling pathways for these effects are not elucidated yet. Given that, some neurosteroids act through the activation of ionotropic glutamate receptors, therefore the effect of DHEA-S on the subunits GluR2 and GluR3of the AMPA receptor was evaluated. Either DHEA-S or a control substance was administered to C57/BL6 mice. Subunit expression of the AMPA receptor was analyzed by Western blotting. Results show that long-term DHEA-S administration toC57/BL6 mice, increases the protein levels of the subunits GluR2 and GluR2/3 of the AMPA receptors located in the hippocampus. Due to the role of AMPA receptor, specifically GluR2subunit in the regulation of intracellular calcium levels, cellular apoptosis, and synaptic plasticity, the study of neurosteroids as a therapeutic strategy in neurodegenerative diseases and cerebrovascular events is very relevant.

The Relationship between Serum DHEA-s Level and TAS in Healthy Adults

BACKGROUND: DHEA is the most abundant steroid hormone secreted from the adrenal cortex and has several roles such as increasing insulin sensitivity, lowering serum lipid, improving obesity, and anti-cancer effects. DHEA levels vary profoundly and levels decline as age increases. But the role of DHEA in the aging process is not yet fully understood. TAS which provides information of one's antioxidant capacity, also decreases with age. In this study, we investigated the relationship between TAS and serum DHEA-s concentration. METHODS: By reviewing the medical records of 197 healthy adults, we determined the serum levels of DHEA-s and TAS according to BMI, pulse pressure, WBC, hs-CRP, A/G ratio, LDL-cholesterol, triglyceride, ferritin, uric acid and total bilirubin. We studied the relationship between the parameters by Pearson correlation coefficients and multiple regression analysis. RESULTS: The mean level for TAS and serum DHEA-s was 1.20 mml/L and 177.3 ug/mL, respectively. Serum DHEA-s correlated positively with TAS (r=0.44) and negatively for age (r=-0.44). WBC triglyceride and uric acid also showed statistically significant correlations. But, according to multiple regression analysis DHEA-s only correlated with TAS and age. CONCLUSION: Serum DHEA-s correlates positively with TAS.

The Relationship between Serum DHEA-s Level and TAS in Healthy Adults

BACKGROUND: DHEA is the most abundant steroid hormone secreted from the adrenal cortex and has several roles such as increasing insulin sensitivity, lowering serum lipid, improving obesity, and anti-cancer effects. DHEA levels vary profoundly and levels decline as age increases. But the role of DHEA in the aging process is not yet fully understood. TAS which provides information of one's antioxidant capacity, also decreases with age. In this study, we investigated the relationship between TAS and serum DHEA-s concentration. METHODS: By reviewing the medical records of 197 healthy adults, we determined the serum levels of DHEA-s and TAS according to BMI, pulse pressure, WBC, hs-CRP, A/G ratio, LDL-cholesterol, triglyceride, ferritin, uric acid and total bilirubin. We studied the relationship between the parameters by Pearson correlation coefficients and multiple regression analysis. RESULTS: The mean level for TAS and serum DHEA-s was 1.20 mml/L and 177.3 ug/mL, respectively. Serum DHEA-s correlated positively with TAS (r=0.44) and negatively for age (r=-0.44). WBC triglyceride and uric acid also showed statistically significant correlations. But, according to multiple regression analysis DHEA-s only correlated with TAS and age. CONCLUSION: Serum DHEA-s correlates positively with TAS.

The Study about the change of Fetal Adrenal Gland Hormone during Pregnancy and Labor / 대한산부인과학회잡지

OBJECTIVE: Fetal adrenal gland hormone was known to have close association with fetal weight and labor pain in response to fetal stress. Our purpose was to analyze the correlation between cortisol, DHEA-S in cord blood and obstetric status of fetuses. METHODS: We collected cord blood samples from 60 neonates immediately after delivery of baby. Six cases were complicated by intrauterine growth restriction and fourteen patients were complicated by preeclampsia. We measured cortisol, DHEA-S levels by using the RIA commercial Kits for the ELISA. We performed statistic analysis of the data on the SPSS computer program. RESULTS: Cortisol was significantly higher in intrauterine growth restriction (23.4 +/- 16.2 microgram/dL) statistically than control group (13.7 +/- 9.5 microgram/dL) (P=0.022). Cortisol and DHEA-S had negative correlation each other (P=0.025). DHEA-S was significantly lower in intrauterine growth restriction (1202.2 +/- 1005.9 ng/ mL) than control group (1714.8 +/- 978.6 ng/mL) (P=0.01). DHEA-S had increased with advancing gestational age (P=0.03) and fetal weight (P=0). CONCLUSION: Cord blood cortisol level and DHEA-S level has close association with intrauterine growth restriction and negative correlation with each other.

Relationship between Stress and Dehydroepiandrosterone Sulfate in Umbilical Blood of Pre-birth / 中国心理卫生杂志

Objective:To explore the correlation of stress, coping style and the concentration of dehydroepiandrosterone sulfate in umbilical blood between preterm birth and normal birth.Methods:46 with preterm birth and 42 normal birth controls were assessed with Life Event Scale (LES) and Defense Style Questionnaire (DSQ) at 28th-week of pregnancy. The concentrations of DHEA-s in umbilical blood were determined by ELISA for the two groups. Results:Of the scales of malignant life events (frequency: 1.24?0.74 vs 1.04?0.03; strength: 56.21?4.03 vs 44.35?1.06)、immature and middle type defense style scales (4.24?0.13 vs 3.55?0.11; 3.86?0.08 vs 3.64?0.06), and DHEA-s (0.72?0.02 vs 0.33?0.03) there were significant difference between preterm birth and normal birth controls (P

Relation of plasma dehydroepiandrosterone sulfate(DHEA-S) to cardiovascular risk factors

BACKGROUND: Few studies have been done regarding the level of DHEA which is influenced by age, and their effect on cardiovascular disease and prevention of cancer. It is a well known fact that the level of DHEA is decreased with age and the aging is not a correctable risk factor for cardiovascular disease. The aim of this study was to identify plasma DHEA-S change by age and to find out if there was any correlation with serum DHEA-S and cardiovascular risk factors. METHODS: The author collected blood from 85 males and 80 females who had no particular disease history and no specific findings on physical examination. If there were any changes of DHEA according to age, we analyzed the correlation of DHEA with cardiovascular risk factors such as blood pressure, total cholesterol, triglyceride, low density lipoprotein, high density lipoprotein and obesity index (body mass index, waist/hip ratio). RESULTS: In both males and females, plasma DHEA-S level peaked at third dacades and the concentration of DHEA was significantly decreased according to aging (p<0.01). In males, DHEA-S showed no correlations with cardiovascular risk factors. In females, DHEA-S showed negative correlations with systolic blood pressure, diastolic blood pressure, total cholesterol, and low density lipoprotein. Also, high density lipoprotein positively correlated with DHEA-S. These correlations in female subjects, however, disappeared after multiple regression analysis. CONCLUSION: In both males and females, plasma DHEA-S was significantly decreased with advancing age. There was no significant correlation between DHEA-S and cardiovascular risk factors in both men and women.

Fetal Plasma Cortisol and Dehydroepiandrosterone Sulfate in Pregnancy and Term Parturition / 대한산부인과학회잡지

OBJECTIVE: The role of steroid hormones in the control of human parturition has been a subject of debate. The objective of the study was to examine if changes in fetal plasma cortisol or dehydroepiandrosterone sulfate (DHEA-S) are associated with human term parturition. METHODS: Fetal plasma cortisol and DHEA-S were measured in 374 singleton pregnancies delivered at term. Umbilical cord blood was obtained from patients in the following 6 groups: 1) preterm gestations undergoing cordocentesis for clinical indications before 36 weeks of gestation (n=93), 2) women undergoing cordocentesis for clinical indications after 36 weeks of gestation (n=9), 3) elective cesarean section (C/S) at term without labor (n=140), 4) C/S at term with early labor (cervical dilatationp36 weeks) but did not increase during active labor; 4) The cortisol/ DHEA-S ratio (stress index) increased with advancing gestation and with active labor at term. CONCLUSION: Human parturition at term is associated with an increase in fetal plasma cortisol and cortisol/DHEA-S ratio, but not DHEA-S.

Clinical Features and Serum Dehydroepiandrosterone Sulfate and Total Testosterone Levels in Female Patients with Androgenetic Alopecia / 대한피부과학회지

BACKGROUND: The development of androgenetic alopecia is thought to be caused by increased androgen action on the hair follicles in the genetically predisposed person. Although most reports about dehydroepiandrosterone sulfate(DHEA-S) and total testosterone in female androgenetic patients are within normal limits, there are some controversies about comparing the mean values of the patients with those of the normal control group. OBJECTIVE: The purpose of this study was to compare the mean value of plasma DHEA-S and total testosterone of the patients with those of normal controls according to their ages, and evaluate relations between hormone levels and clinical type and hyperandrogenic symptoms(HAS). METHOD: We examined 60 female patients with androgenetic alopecia for clinical types, symptoms, family histories, and other systemic diseases. The mean value of the patients were compared with those of 42 normal controls according to ages, clinical types, and HAS. RESULTS: 1. Forty two cases(70.0%) were Ludwig type I, 16 cases(26.7%) were type II, 2 cases(3.3%) were type III. 26 cases(43.3%) were between 20-29 years, 19 cases(31.7%) were between 30-39 years. 2. Common age of onset was between 20-29years(23 cases, 38.3%) and below 19 years old(21 cases, 35.0%). 3. There were 32 cases(53.3%) who showed HAS and seborrhea was the most common symptom (20 cases). 4. There were 33 cases(55.0%) who showed a family history and the father was the most common relative(19 cases). Family history of first degree relative was 31 cases(51.7%). 5. Plasma DHEA-S levels of both patients and control group were all within normal limits, and there were no significant differences in the mean values between the patients ( 1633.03+/-736.31 ng/ml) and normal controls(1764.72+/-690.94 ng/ml). There were also no difference between the patients and controls according to their ages. 6. In total testosterone, 7 out of 60 patients and 3 out of 42 normal controls were beyond the normal limit and there were no significant differences in the mean values between the patients(0.548+/-0.386 ng/ml) and normal controls(0.563+/-0.501 ng/ml). There were also no differences between the patients and controls according to their ages. 7. There was no significant difference in the mean value of DHEA-S and total testosterone among type I, types II & III, and normal controls. 8. There was no significant difference in the mean value of DHEA-S and total testosterone among patients with HAS, without HAS, and normal controls. CONCLUSION: There were no significant differences in the mean values of plasma DHEA-S and total testosterone between the patients and normal controls and no significant differences in the mean values of hormone levels according to clinical type and HAS.

Relationship between the concentration of fetal total bile acid and fetal adrenocortical dysfunction in intrahepatic cholestasis of pregnancy / 中华围产医学杂志

Objective To evaluate the association of fetal total bile acid (TBA) concentration with fetal adrenocortical dysfunction in intrahepatic cholestasis of pregnancy(ICP). Methods The concentration of TBA, cortisol and DHEA-S in the cord blood were measured in 20 fetuses with maternal ICP (ICP group) and 22 fetuses of normogravidas (control group) after elective cesarean section. The cord blood TBA concentration was investigated by enzyme method and the cord concentration of cortisol and DHEA-S by radioimmunoassay. Results The cord TBA concentration in ICP group was significantly higher than that of controls [(8.93?3.16)mmol/L vs (4.33?1.51)mmol/L, P0.05)]. The cord blood level of cortisol,DHEA-S and the ratio of DHEA-S over cortisol were correlated with the cord blood TBA concentration (r 1= 0.87,r 2=-0.88,r 3=-0.84,P

Relationship of Dehydroepiandrosterone Sulfate, Sex Hormone Binding Globulin and Carotid Atherosclerosis in Women / 대한내분비학회지

BACKGROUND: Cardiovascular disease is the major cause of morbidity and mortality. It is well known that androgen and sex hormone-binding globulin(SHBG) in women were associated with risk factors for cardiovascular disease such as obesity, central fat accumulation and atherogenic lipid profile. High resolution B-mode ultrasonography can visualize directly both luminal and vessel wall characteristics, and ultrasonic measurement of carotid intima-medial thickness(IMT) can be used to investigate the atherosclerosis of coronary, cerebral and peripheral arteries. The aim of this study was to evaluate the relationship between the serum dehydroepiandrosterone sulfate(DHEA-S) and SHBG, and body fat distribution pattern, cardiovascular risk factor and carotid atherosclerosis in women. METHODS: Blood pressure, fasting serum glucose, insulin, lipid profile, SHBG and DHEA-S were measured. Body fat distribution pattern was assessed by waist to hip ratio, waist to thigh ratio and subscapular to triceps skin fold thickness ratio, faf mass measured by bioelectric impedance analyzer, subcutaneous fat area, visceral fat area, and visceral to subcutaneous fat area ratio(VSR) at the level of umbilicus using the computed tomography. The IMT of the carotid artery was measured by high resolution B mode ultrasound as a marker of atherosclerosis. RESULTS: 1) There was no significant difference of age-adjusted values of carotid IMT among subjects with NGT(0.426+/-0.09mm), IGT(0.46+/-10.09mm) and NIDDM(0.453+/-0.11mm). 2) In postrnenopausal women, carotid IMT was greater(0.484+/-0.11mm, p=0.05) than premenopausal women(0.426+/-0.08mm), but it was not significant after age adjustment. Serum SHBG and DHEA-S levels in postmenopausal women were significantly lower(p<0.05) than premenopausal women, but they were not significant after age adjustment. 3) Carotid IMT was significantly correlated with age(r=0.37, p<0.01), fasting serum glucose (r=0.32, p<0.01), total cholesterol(r= 0.25, p<0.05), LDL-cholesterol(r=0.26, p<0.05), visceral fat area(r=0.35, p<0.01) and VSR(r=0.31, p<0.05). 4) By the stepwise multiple regression analysis, carotid IMT was positively and significantly associated with age(p<0.01) and serum DHEA-S concentration(p<0.05) in dent of age, body mass index, waist to hip ratio, fasting serum glucose. CONCLUSION: Carotid IMT was associated with age and serum DHEA-S concentration in women without cardiovascular disease. Therefore, serum DHEA-S may be one of risk factors for cardiovascular disease in women. However, we could not conclude its cause-result relationship because of cross sectional nature of our study, and prospective study will be needed.

Changes in Plasma Dehydroepiandrosterone-Sulfate ( DHEA-S ) Level & DHEA-S / cortisol Ratio by Age in Healthy Korean / 대한내분비학회지

BACKGROUND: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. METHODS: Healthy Korean (men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs (such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4 (Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. RESULTS:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels (men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women (men; y=-3.152 * +292.6, r2= 0.8459, P<0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P< 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA-S/cortisol ratio. CONCLUSION: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu (aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index.

Plasma Levels of Dehydroepiandrosterone Sulfate ( DHEA - S ) and Total Testosterone in the Patients with Female androgenetic Alopecia / 대한피부과학회지

BACKGROUND: It has been known that androgenetic alopecia in both male and female is due to the role of the androgen hormone in the hair follicle, but the level of the androgen hormone in the plasma is controversial in relation with the development of the alopecia. OBJECTIVE: We have tried the clarify if there is any or no difference in the plasma levels of the hormones between the normal female and the patient with alopecia, because the range of the plasma DHEA-S and total testosterone in the normal female is very wide. METHODS: Plasma levels if the hormones were examined in 22 patients with female androgenetic alopecia and 20 normal females. RESULTS: 1. In DHEA-S, 21(95.5%) aut of 22 female androgenetic alopecia patients were in the normal range while 20(100%) of the 20 normal females were all within the normal range. However the mean value of the hormone(88.89+101.41 g/dl) in the patient group was significantly higher than that(82.18+44.03 g/dl) of the normal group(p<0.05). 2. In total testosterone, 17(94.4%) out of the 18 patents were in the normal range while 20 (100%) of 20 normal females were all within the normal range. However the mean value of the hormone(0.22+0.20ng/ml) in the patient group was significantly higher than that(0.08+0.09ng/ml) of the normal group(p<0.05). 3. As for distribution of DIEA S according to the age, the value of the patient group was higher than that of the normal group in all age groups studied. In the distribution of total testosterone according to age, there was no difference between the normal and the patient in the twenties. After the twenties, the testosterone levels were higher in the patients than the normal females. CONCLUSION: There were significant differences in mean values of plasma DHEA-S and total testosterone between the patients and the normal females although individuals in both groups were largely wit,hin normal range of the hormones.

A Study on Plasma Levels of Testosterone and Dehydroepiandrosterone - sulfate ( DHEA - S ) in the Patients with Acne Vulgaris / 대한피부과학회지

This study was performed to detect the hormonal abnormalities and to investigate the relationship between the levels of plasma testosterone and DHEA-S in 21 female patients with acne vulgaris using radioimmunoassay. The results were as follows : 1. The levels of plasma testosterone in patients group and control group(n=9) were 352+/-11.8 ng/dl, 223+/-9.7 ng/dl, respectively, and significantly elevated in patient group(p < 0.01), 2. The levels of plasma DHEA-S in patient group and control group were 286.6+/-126.8 pg/dl, 238.6+/-60.0 pg/dl, respectively, and did not show statistical significance. 3. The levels of plasma testosterones in patient subgroups classified by modified Pillsbury method were as follows minor group 32.6+5.3 ng/dl, mild group 33.4+/-15,5 ng/dl, moderate group 39.6+/-12.6 ng/dl. The testosterone levels in all the subgroups increased significantly than those in control group(352-t11.8 ng/dl) (p<0.05, p<0.05 p < 0.01) 4. The levels of plasrna DHEA-S in patient subgroups were follows minor group 242.5+/-412 pg/dl, mild group 263.9+/-166.1 pg/dl, moderate group 353.4+/-1273 pg/dl. The DE3EA-S levels in moderate group increased significantly than those in cotrol (238,6+/-60,0 pg/dl) and minor group(p<0.05). 5. There was no correlation between the plasma testosterone levels and the plasma DHEA-S levels in the acne patients.