DNA molecular identification of Polyrhachis dives medicinal materials / 药学学报

In the study, specific primers were designed based on the CO Ⅰ gene sequence of Polyrhachis dives. By optimizing the genomic DNA extraction method and amplification conditions, we established an efficient, specific, and accurate DNA molecular identification method for Polyrhachis dives. In this method, the length of the target fragment was 294-308 bp, and the other counterfeits had no target bands. In this paper, the specific identification method of the origin of Polyrhachis dives established can be used to identify the medicinal materials of Polyrhachis dives accurately.

Identification of Bupleurum scorzonerifolium and its adulterants / 中国药学杂志

OBJECTIVE: To explore a set of identification methods of discriminating Bupleurum scorzonerifolium from its adulterant Bupleuru bicaule. METHODS: Methods of morphological comparison of plant samples with specimens, macroscopic identification of the roots, TLC identification of the root extracts, and DNA molecular identification by ITS2 sequence were applied. RESULTS: There were distinctive differences in the morphological, macroscopic, and TLC characteristics between B. chinense and B. scorzomerifolium. The DNA of ITS2 sequence was able to be identified by measuring their mean intraspecific genetic distance (K-2-P distance) which was lower than their mean interspecific genetic distance. In the cluster dendrogram, both of them showed monophyletic property and could be distinguished from other adulterants. CONCLUSION: On the basis of comparison of Bupleurum scorzonerifolium and its adulterant Bupleurum bicaule, the main identification features were summarized by a combination of original plant morphology, macroscopic identification of the roots, TLC identification, and DNA molecular identification methods. DNA identification technology is an important supplement of the traditional identification methods, which may have great potential and application prospect in origin identification of medicinal plants.

Research Progress on DNA Molecular Identification of Plants in the Genus Chrysanthemum / 世界科学技术-中医药现代化

This review summarized the research status on DNA molecular identification of plants in the genus Chrysanthemum. Some case studies on representative DNA molecular identification techniques, which included ge-nomic in situ hybridization (GISH), DNA molecular markers and DNA barcoding, were described. Simultaneously, the merits, demerits and development of the techniques were discussed. The above work provides evidence for the identi-fication and resource utilization of plants in the genus Chrysanthemum.