RESUMO
Platelet adhesion is a key process in thrombosis. Anti-platelet adhesion effect of some Chinese medicines for promoting blood circulation and removing blood stasis (PBCRBS) has been reported, but their relative efficacies as a whole and specific targets remained unclear. This paper combined activity screening, drug compatibility analysis, pathway clustering, target prediction, and molecular docking to explore the mechanism of anti-platelet adhesion by PBCRBS Chinese medicine. Screening the activity of anti-platelet adhesion of 58 commercially available PBCRBS Chinese patent medicines showed that about 50.0% significantly inhibit ADP-induced platelet adhesion in vitro, and about 96.6% significantly inhibit thrombin-induced platelet adhesion in vitro. The animal experiment involved was approved by the Animal Ethics Committee of Tianjin International Biomedical Research Institute. Combined with the auxiliary platform for TCM (V2.0) inheritance showed that the compatibility of Danshen-Chuanxiong was used most frequently among the top 20 active proprietary Chinese patent medicines. IPA network analysis revealed that IL-1, APP and CCL2 might be the key targets for anti-platelet adhesion function of Danshen-Chuanxiong against atherosclerosis, neuroinflammation and chemokine signaling pathways as the main mechanisms. Molecular docking analysis confirmed the interaction between one of the active compounds shared by Danshen and Chuanxiong, i.e. chlorogenic acid, with its target CCL2. This study provides TCM theory guidance and experimental support for targeting platelet adhesion in anti-thrombosis therapy by Chinese medicine for promoting blood circulation and removing blood stasis.
RESUMO
Objective To study the Danshen Chuanxiong injection on the expression of serum inflammatory factors in patients with severe burn ,and the preventive effect on SIRS and multiple organ dysfunction syn-drome (MODS) .Methods 56 patients with the severe burn admitted in the hospital from January 2015 to Jan-uary 2016 were enrolled in the study ,and divided randomly into the control group and the observation group . The control group received routine treatment ,while the observation group received routine treatment as well as intra verous drip infusion treatment of Danshen Chuanxiong at the same time .The clinical efficacy in two groups and the incidence rates of SIRS and MODS were evaluated .The changes of the expression of serum C reactive protein (CRP) ,interleukin -10 (IL-10) and IL-6 before and after treatment ,and their correlations with the severity of SIRS and MODS was analyzed .Results The clinical efficacy of the observation group was better than that of the control group ,and the difference was statistically significant (P<0 .05) .The incidence rate of SIRS in the observation group was lower than that in the control group and the difference was statisti-cally significant (P<0 .01);the illness condition of the patients with SIRS in the observation group were less serious than those in the control group ,and the differences was statistically significant (P<0 .01) .There was no statistically significant difference in the incidence rates of MODS between the observation group and the control group (P>0 .05) ,but the conditions of MODS patients in the observation group were obviously lessheavier than those in the control group ,and the differences were statistically significant (P< 0 .05) .After treatment ,the indexes of IL-6 ,CRP and IL-10 were significantly improved ,and the differences were statistical-ly significant (P<0 .05) .After treatment ,IL-6 and CRP levels in the observation group were lower than those in the control group ,while the IL-10 level was higher than that in the control group ,and the differences were statistically significant ( P< 0 .05) .SIRS had a relatively high correlation with IL-6 ,CRP and IL-10 ( P<0 .05) ,and there was a relatively high correlation between MODS and IL-6 and CRP (P<0 .05) ,and there was a moderate correlation between MODS and IL-10 (P<0 .05) .Conclusion Danshen Chuanxiong injection can control the inflammatory response in patients with severe burns ,improve the expression level of serum IL-6 , CRP and IL-10 ,and it has a clear positive effect on preventing the occurrence of SIRS and MODS and allevia-ting the severity of SIRS and MODS ,which is worth of clinical attention .