Study on the anti-tumor effect of CD30 CAR-T cells based on multi-chain structure / 安徽医科大学学报

Objective @#To develop a CD30-targeted CAR-T cell drug based on the multi-chain chimeric antigen re- ceptor T cells (CAR-T) of the bridging protein DAP12 , and to study the in vitro and in vivo preclinical efficacy of CD30 CAR-T on Hodgkin lymphoma tumor cells .@*Methods @#Through gene synthesis and molecular cloning tech- niques , a CAR plasmid targeting CD30 was designed and constructed , and the obtained lentivirus was packaged . The T cells were transfected with the lentivirus , where the multi-chain CAR-T targeting CD30 was the CD30 - KIRS2/Dap12-BB group , the single-chain second-generation CAR-T was the CD30-41BBζgroup , and the T cells without virus infection were the NTD group . The positive rate of CAR was detected by flow cytometry , the cytotoxic- ity of the cells was detected by lactate dehydrogenase (LDH) release assay , the secretion level of the cytokine in- terferon γ(IFN-γ) was detected by enzyme-linked immunosorbent assay ( ELISA) , and the antitumor activity of CD30 CAR-T in mice was further detected by a mouse xenograft tumor model . @*Results @#A comparison was made between the multi-chain CAR-T targeting CD30 and the single-chain second-generation CAR-T. It was found that the antitumor effect of the multi-chain CAR-T was similar to that of the single-chain CAR-T. However , it was worth noting that the IFN-γsecretion level of the multi-chain CAR-T was higher (P < 0 . 001) . More importantly , in the mouse tumor model experiment , the multi-chain CAR-T achieved complete tumor regression . @*Conclusion @#The multi-chain CAR-T targeting CD30 is superior to the traditional single-chain CAR-T in terms of antitumor activity .

Trombocitopenia severa inducida por un oclusor para DAP: reporte de caso en tecnovigilancia

Introducción: El ductus arterioso persistente (DAP) es una cardiopatía congénita de especial interés. La oclusión intervencionista es el tratamiento de elección, no obstante, el dispositivo médico "oclusor para DAP", usado en este procedimiento, puede dar lugar a la aparición de eventos adversos de rara frecuencia como hemorragia, trombocitopenia, entre otros. Reporte de caso: Se presenta un caso de una paciente pediátrica con trombocitopenia severa posterior a la intervención. Durante su hospitalización, la paciente mantuvo niveles bajos de plaquetas hasta que logra su recuperación y sale de alta sin una nueva manifestación hematológica. El caso fue reportado como sospecha de incidente adverso asociada al oclusor para DAP. Conclusión: Al realizarse el análisis causa-raíz, se concluye que el tamaño del oclusor para DAP podría ser la principal causa de la trombocitopenia. El presente caso es una muestra que los dispositivos médicos pueden causar eventos adversos severos, por lo que, es necesaria la implementación de tecnovigilancia, principalmente a los dispositivos médicos de alto riesgo.

Introduction: Patent ductus arteriosus (PDA) is a congenital heart condition of special interest. Interventional occlusion is the treatment of choice; however, a PDA occlude, used in this procedure, is a medical device that rarely could lead to adverse events such as hemorrhage, thrombocytopenia, etc. Case report: We present a case of a pediatric patient with severe thrombocytopenia after surgery. During her hospitalization, platelet levels remained lower and, finally, she was fully recovered without any hematological manifestation. The case was reported as a suspected adverse incident related to PDA occlude. Conclusion: After performing a cause-root analysis, we concluded that the size of the PDA occlude may be the main cause of thrombocytopenia. This case demonstrates that medical devices could lead to adverse events. Thus, it is important for clinicians to implement medical device surveillance, mainly for high-risk medical devices.

Novel CD19-KIRS2/Dap12-BB CAR-T Treatment for 3 Patients with Relapsed and Refractory B-Cell Tumors / 中国实验血液学杂志

OBJECTIVE@#To investigate the safety and efficacy of novel CD19-KIRS2/Dap12-BB chimeric antigen receptor T cells (CAR-T cells) in the treatment of relapsed/refractory B-cell malignancy (R/R BCM).@*METHODS@#Three patients with R/R BCM treated with novel CD19-KIRS2/Dap12-BB CAR-T cells from June 2020 to November 2020 were enrolled, including 1 case of B-cell acute lymphoblastic leukaemia (B-ALL) and 2 cases of non-Hodgkin's lymphoma (NHL), and the efficacy and adverse reactions were observed.@*RESULTS@#After CAR-T cells infusion, patient with B-ALL achieved complete remission (CR) and minimal residual disease (MRD) turned negative, and 2 patients with NHL achieved partial remission (PR). Grade 2 cytokine release syndrome (CRS) occurred in B-ALL patient, grade 1 CRS occurred in 2 NHL patients, and grade II to IV hematologic adverse reactions occurred in 3 patients, all of which were controllable and reversible. The progression-free survival (PFS) of the 3 patients was 143, 199, and 91 days, and overall survival (OS) was 282, 430, and 338 days, respectively.@*CONCLUSION@#The novel CD19-KIRS2/Dap12-BB CAR-T cells in treatment of 3 patients with R/R BCM have significant short-term efficacy and controllable adverse reactions, but the long-term efficacy needs to be further improved.

Actitudes hacia la muerte expresadas por jóvenes en contexto COVID-19 en la región de Tarapacá / The death attitudes expressed by young adults from Tarapacá region in COVID-19 context / Atitudes de jovens da região de Tarapacá em relação a morte no contexto de COVID-19

Resumen Esta investigación analizó las actitudes hacia la muerte expresadas por un grupo de adultos jóvenes en contexto COVID-19. Los participantes fueron 10 jóvenes residentes en la región de Tarapacá (60% mujeres, 40% hombres) entre 18 a 29 años. La metodología tuvo un enfoque mixto de preponderancia cualitativa y diseño secuencial. En la primera etapa, se administró el Perfil Revisado de Actitudes hacia la muerte [PAM-R] para medir cinco actitudes: Miedo a la muerte; Evitación de la muerte; Aceptación de acercamiento; Aceptación de escape; y Aceptación neutral. Posteriormente, se aplicaron entrevistas semiestructuradas que profundizaron los resultados, mediante la exploración de las percepciones, emociones y pensamientos de los participantes durante la pandemia. Finalmente, se triangularon los datos cuantitativos y cualitativos para obtener una mayor validez. Los resultados señalan a estas actitudes como un fenómeno contextual, cuya expresión depende de diversos elementos personales y del entorno, siendo los más importantes los miedos asociados a familiares. En jóvenes, la muerte fue comprendida como una realidad lejana. Esto influyó en una alta neutralidad y bajo miedo a la muerte propia. El contexto COVID-19 actuó como un factor influyente en las actitudes, particularmente en el miedo al contagio o a la posibilidad de morir. Si bien los jóvenes reconocieron la peligrosidad del virus, sus necesidades emocionales-afectivas se vislumbraron como problemas más urgentes. Por lo tanto, los miedos en contexto COVID-19 no evitaron la exposición al contagio en compañía de amigos. Estas conductas fueron significadas como medidas de autocuidado psicológico, particularmente en jóvenes con Aceptación de Escape.

Abstract This research analyzed the attitudes towards death expressed by a group of young adults in a COVID-19 context. The participants were 10 young residents in the Tarapacá region (60% women, 40% men) between 18 and 29 years old. The methodology had a mixed approach of qualitative preponderance and sequential design. First, the Death Attitude Profile-Revised [DAP-R] was administered to measure five attitudes: Fear of Death, Death Avoidance, Approach Acceptance, Escape Acceptance, and Neutral Acceptance. Subsequently, semi-structured interviews were applied that deepened the results, by exploring the perceptions, emotions and thoughts of the participants during the pandemic. Finally, the quantitative and qualitative data were triangulated to obtain greater validity. The results point to these attitudes as a contextual phenomenon, the expression of which depends on various personal and environmental elements, the most important being the fears associated with family members. In young people, death was understood as a distant reality. This influenced a high neutrality and low fear of own death. The COVID-19 context acted as an influencing factor in attitudes, particularly in fear of contagion or the possibility of dying. Although the young people recognized the dangerousness of the virus, their emotional-affective needs were seen as more urgent problems. Therefore, fears in the COVID-19 context did not prevent exposure to contagion in the company of friends. These behaviors were signified as measures of psychological self-care, particularly in young people with Escape Acceptance.

Resumo Este estudo teve como objetivo analisar as atitudes de um grupo de jovens adultos em relação a morte no contexto de COVID-19. Nela participaram 10 jovens que vivem na região de Tarapacá (60% mulheres, 40% homens) os quais tinham entre 18 e 29 anos. A metodologia da pesquisa teve uma abordagem mista de preponderância qualitativa e desenho de investigação sequencial. Na primeira etapa, foi administrado o Perfil Revisado de Atitudes em Relação a Morte [PAM-R] para medir cinco atitudes: Medo da morte; Evitar a morte; Aceitação de abordagem; Aceitação de fuga; e aceitação neutra. Posteriormente, foram aplicadas entrevistas semi-estruturadas que aprofundaram os resultados, explorando as percepções, emoções e pensamentos dos participantes durante a pandemia. Por fim, os dados quantitativos e qualitativos foram triangulados para obter maior validade. Os resultados apontam essas atitudes como um fenômeno contextual, cuja expressão depende de diversos elementos pessoais e do meio ambiente, sendo o mais importante deles, os medos associados aos parentes. Nos jovens, a morte era entendida como uma realidade distante. Isso influenciou uma alta neutralidade e pouco medo da propia morte. O contexto de COVID-19 atuou como um fator influente nas atitudes, particularmente no medo de contágio ou na possibilidade de morrer. Enquanto os jovens reconheciam o perigo do vírus, suas necessidades emotivas e afetivas eram vistas como problemas mais urgentes. Portanto, os temores no contexto de COVID-19 não impediram a exposição ao contágio na companhia de amigos. Esses comportamentos foram considerados como medidas de autocuidado psicológico, particularmente em jovens com Aceitação da Fuga da morte.

Actitudes hacia la muerte expresadas por jóvenes en contexto COVID-19 en la región de Tarapacá

Esta investigación analizó las actitudes hacia la muerte expresadas por un grupo de adultos jóvenes en contexto COVID-19. Los participantes fueron 10 jóvenes residentes en la región de Tarapacá (60% mujeres, 40% hombres) entre 18 a 29 años. La metodología tuvo un enfoque mixto de preponderancia cualitativa y diseño secuencial. En la primera etapa, se administró el Perfil Revisado de Actitudes hacia la muerte [PAM-R] para medir cinco actitudes: Miedo a la muerte; Evitación de la muerte; Aceptación de acercamiento; Aceptación de escape; y Aceptación neutral. Posteriormente, se aplicaron entrevistas semiestructuradas que profundizaron los resultados, mediante la exploración de las percepciones, emociones y pensamientos de los participantes durante la pandemia. Finalmente, se triangularon los datos cuantitativos y cualitativos para obtener una mayor validez. Los resultados señalan a estas actitudes como un fenómeno contextual, cuya expresión depende de diversos elementos personales y del entorno, siendo los más importantes los miedos asociados a familiares. En jóvenes, la muerte fue comprendida como una realidad lejana. Esto influyó en una alta neutralidad y bajo miedo a la muerte propia. El contexto COVID-19 actuó como un factor influyente en las actitudes, particularmente en el miedo al contagio o a la posibilidad de morir. Si bien los jóvenes reconocieron la peligrosidad del virus, sus necesidades emocionales-afectivas se vislumbraron como problemas más urgentes. Por lo tanto, los miedos en contexto COVID-19 no evitaron la exposición al contagio en compañía de amigos. Estas conductas fueron significadas como medidas de autocuidado psicológico, particularmente en jóvenes con Aceptación de Escape.

This research analyzed the attitudes towards death expressed by a group of young adults in a COVID-19 context. The participants were 10 young residents in the Tarapacá region (60% women, 40% men) between 18 and 29 years old. The methodology had a mixed approach of qualitative preponderance and sequential design. First, the Death Attitude Profile-Revised [DAP-R] was administered to measure five attitudes: Fear of Death, Death Avoidance, Approach Acceptance, Escape Acceptance, and Neutral Acceptance. Subsequently, semi-structured interviews were applied that deepened the results, by exploring the perceptions, emotions and thoughts of the participants during the pandemic. Finally, the quantitative and qualitative data were triangulated to obtain greater validity. The results point to these attitudes as a contextual phenomenon, the expression of which depends on various personal and environmental elements, the most important being the fears associated with family members. In young people, death was understood as a distant reality. This influenced a high neutrality and low fear of own death. The COVID-19 context acted as an influencing factor in attitudes, particularly in fear of contagion or the possibility of dying. Although the young people recognized the dangerousness of the virus, their emotional-affective needs were seen as more urgent problems. Therefore, fears in the COVID-19 context did not prevent exposure to contagion in the company of friends. These behaviors were signified as measures of psychological self-care, particularly in young people with Escape Acceptance.

Este estudo teve como objetivo analisar as atitudes de um grupo de jovens adultos em relação a morte no contexto de COVID-19. Nela participaram 10 jovens que vivem na região de Tarapacá (60% mulheres, 40% homens) os quais tinham entre 18 e 29 anos. A metodologia da pesquisa teve uma abordagem mista de preponderância qualitativa e desenho de investigação sequencial. Na primeira etapa, foi administrado o Perfil Revisado de Atitudes em Relação a Morte [PAM-R] para medir cinco atitudes: Medo da morte; Evitar a morte; Aceitação de abordagem; Aceitação de fuga; e aceitação neutra. Posteriormente, foram aplicadas entrevistas semi-estruturadas que aprofundaram os resultados, explorando as percepções, emoções e pensamentos dos participantes durante a pandemia. Por fim, os dados quantitativos e qualitativos foram triangulados para obter maior validade. Os resultados apontam essas atitudes como um fenômeno contextual, cuja expressão depende de diversos elementos pessoais e do meio ambiente, sendo o mais importante deles, os medos associados aos parentes. Nos jovens, a morte era entendida como uma realidade distante. Isso influenciou uma alta neutralidade e pouco medo da propia morte. O contexto de COVID-19 atuou como um fator influente nas atitudes, particularmente no medo de contágio ou na possibilidade de morrer. Enquanto os jovens reconheciam o perigo do vírus, suas necessidades emotivas e afetivas eram vistas como problemas mais urgentes. Portanto, os temores no contexto de COVID-19 não impediram a exposição ao contágio na companhia de amigos. Esses comportamentos foram considerados como medidas de autocuidado psicológico, particularmente em jovens com Aceitação da Fuga da morte.

O Desenho da Figura Humana com História (DFH-H) e crianças com dificuldades de aprendizagem / Draw-A-Person with A Story (DAP-S) Test and children with learning difficulties / Dibujo de la Figura Humana con História (DFH-H) y niños con dificultades de aprendizaje

As dificuldades de aprendizagem (DA) representam um desafio para as áreas da educação, social e da saúde. O objetivo é avaliar a aplicabilidade do Desenho da Figura Humana com História (DFH-H) na compreensão de crianças com DA. Foram avaliadas, individualmente, 20 crianças da terceira série do Ensino Fundamental público de São Paulo/SP, identificadas pela coordenação da escola como sofrendo algum tipo de DA. Os desenhos foram avaliados segundo os indicadores emocionais (IE) e maturacionais (IM) propostos por Koppitz e também pelo método de livre inspeção do material projetivo, gráfico e narrativo. A média dos IM foi menor, e a dos IE foi superior ao resultado esperado para a idade. A correlação entre os indicadores foi inversa e significante, ainda que moderada. O DFH-H contribuiu para uma compreensão singular das crianças com DA e possibilitou levantar hipóteses sobre os motivos de suas dificuldades, esclarecendo os dados fornecidos pelos IM e IE. (AU)

Learning disabilities (LD) represent a challenge for the educational, social and health fields. The aim was to evaluate the applicability of the Draw-a-Person with a Story (DAP-S) test considering the understanding of children with learning disabilities. Twenty children from the third grade of a Public Elementary School in São Paulo-SP, identified by the school coordination as suffering some type of LD were individually evaluated. Their drawings were analyzed according to the emotional indicators (EI) and maturational indicators (MI) proposed by Koppitz and also through the free inspection method of the projective (graphic/narrative) material. The mean for the MI was lower, and the mean for the EI was higher than the result expected for the age. The correlation between the indicators was inverse and significant, although moderate. The DAP-S contributed to a singular understanding of children with LD and made it possible to raise hypotheses about the reasons for their difficulties, clarifying the data provided by the MI and EI. (AU)

Las dificultades de aprendizaje (DA) representan un reto para las áreas de educación, social y salud. El objetivo es evaluar la aplicabilidad del Diseño de la Figura Humana con Historia (DFH-H) en la comprensión de las DA. Fueron evaluados individualmente veinte niños del tercer año de primaria de una escuela pública de São Paulo-SP, identificados por la coordinación de la escuela como portadores de algún tipo de DA. Sus dibujos fueron evaluados según los indicadores emocionales (IE) y madurativos (IM) propuestos por Koppitz y por el método de libre exploración del material proyectivo, gráfico y narrativo. La media del IM fue menor y la media del IE fue mayor que el resultado esperado para la edad. La correlación entre los indicadores fue inversa y significativa, aunque moderada. El DFH-H contribuyó a una comprensión singular de los niños con DA y permitió plantear hipótesis sobre sus dificultades, aclarando los datos aportados por los IM y IE. (AU)

Recent advances in triggering receptor expressed in myeloid 2 and its role in Alzheimer's disease / 中华神经医学杂志

Mutations in the triggering receptor expressed in myeloid 2 (TREM2) gene have recently been found to be closely related to the development of Alzheimer's disease (AD) and other neurodegenerative diseases. However, the detailed mechanism underlying TREM2's participation in the pathogenesis of AD and other diseases has yet to be fully elucidated. In this review, we discuss current understanding on the structure and function of TREM2, the relevance between TREM2 and AD, and the possible causes of AD by TREM2 mutations.

Eatimation of effective doses from a dental cone beam computed tomographic scanner / 中华放射医学与防护杂志

Objective To calculate effective dose based on the dose-area Product (DAP) measured in dental cone beam computed tomograhic (CBCT) scanning and using a conversion coefficient.Methods The method of the DAP was used to calculate the effective dose from KODAK 9500 dental CBCT scanner with fields of view (FOV) of 16 cm×15 cm and 12 cmu×8 cm.The DAP-values (mGy · cm2) were measured using a transmission ionization chamber connected to an electrometer (Doseguard 100,RTI Electronics AB,Sweden) and the chamber was placed directly on the exit of the X-ray tube.All measurements were repeated three times and averaged on each FOV data.The conversion coefficient of 0.080 μSv/mGy · cm2 was used to estimate the effective dose on the basis of DAP values,which was quoted from a literature.Results DAP values were found between the two FAOs to vary from 512 to 1 083 mGy · cm2,and the effective doses calculated with 0.080 μSv/mGy · cm2were 41 to 87 μSv.The effective doses given in this paper was lower than those reported in other literatures.Conclusions DAP measurement was found to be well defined and easy-to-use method of determining effective dose for some CBCT Units.However,determination of specific conversion coefficient in the CBCT must be further developed.

The relationship between inflammatory factor expression and cell apoptosis in gingival tissue of the subjects with diabetes associated periodontitis / 实用口腔医学杂志

Objective:To study the relationship between IL-1β and TNFα expression and cell apoptosis in gingival tissue of the subjects with diabetes associated periodontitis(DAP).Methods:20 cases of DAP(group DAP) and 20 cases of health controls(group H)were included.The cell apoptosis and the ultrastructural changes in gingival tissue were observed by Tunnel staining and transmission electron microscope (TEM).IL-1β and TNFα expression in gingival tissue were detected by immunohistochemical staining.SBI,GI,PD and AL of the subjects were measured.The relationship between the level of IL-1β,TNFα and the cell appotosis was analyzed.Results:Apoptosis was obvious in prickle cells and basal cells of gingival tissue of DAP group.The percentage of apoptosis cells of DAP group was significantly higher than that of group H(P＜0.01).The expression of IL-1β and TNFα in group DAP was significant higher than that of group H (P＜0.01),and the mainly positive expression cells were macrophages,plasmocytes and lymphocytes.Conclusion:IL-1β and TNFα play a role in cell apoptosis in the gingival tissue of the patients with DAP.

Fijación de carbono atmosférico en la biomasa total de sistemas de producción de cacao en el departamento del tolima, colombia

Los sistemas de uso del suelo con leñosas perennes, como cacaotales, mitigan el cambio climático, al capturar CO2 atmosférico. El objetivo del estudio fue estimar la fijación de carbono en biomasa total en los sistemas de producción de cacao, dominantes del Tolima, Colombia. El estudio, se realizó en Rovira y Falan, empleando un diseño experimental, completamente al azar, con seis sistemas de producción de cacao: monocultivo, sistema agroforestal (SAF) con maderables, SAF con aguacate, SAF con cítricos, SAF con frutales, y SAF con maderables y frutales y tres repeticiones. Se establecieron dos parcelas de muestreo rectangulares, de 1.000m² por unidad de muestreo, donde se identificaron y midieron los árboles con dap ≥ 10cm (diámetro del tronco a la altura del pecho -dap- y altura total -ht). Se establecieron dos sub-parcelas de 256m², por parcela principal, para medir arbustos de cacao: ht y diámetro del tronco, a 30 cm de altura (D30). Se emplearon modelos alométricos, para estimar la biomasa. El SAF con maderables y frutales y SAF con frutales presentaron la mayor biomasa total (122,0 y 72,5t/ ha). En el SAF con maderables y frutales, se evidenció el mayor almacenamiento de carbono, con 61,0t C/ha, mientras que la mayor tasa de fijación de carbono fue de 17,7t/ha/ año, para el SAF con cítricos. Los resultados sugieren que la producción de cacao, que incluyen otras especies vegetales, diversifica la producción e incrementa la seguridad alimentaria y la generación de servicios ambientales, tal como la captura de carbono atmosférico.

The land use systems with woody perennials species, such as cacao plantations, mitigate climate change by capturing atmospheric CO2. The aim of the study was to estimate the fixation of atmospheric carbon in total biomass in the most dominant cocoa production systems in Tolima, Colombia. The study was carried out in Rovira and Falan using a completely randomized experimental design with six cocoa production systems: monoculture, agroforestry system (SAF) with timber trees, SAF with avocado, SAF with citrus species, SAF with fruit trees and SAF with timber and fruit species with three replications. Two rectangular sampling plots 1000m² were established by sampling unit where trees with diameter of trunk at breast height (dbh) ≥ 10cm were identified and measured (dbh and total height - th). Two subplots of 256 m² by main plot, for measuring cocoa bushes were established: ht and trunk diameter at 30cm height (D30). Allometric models to estimate biomass were used. The AFS with timber and fruit species and AFS with fruit species had the greatest biomass (122.0 and 72.5t/ha). In the AFS with timber and fruit species presented the highest carbon storage in biomass with 61.0t C/ha; whereas the greatest carbon fixation rate was 17.7 t/ha/year in AFS with citrus species. The results suggest that cocoa plantations that include other plant species diversify the production and increase food security and generation of environmental services, such as capturing atmospheric carbon.

Is the NMDA Receptor of the CA1 Region Participated in the Amnesic Effect of Harmane?

Aims: In the present study, we investigated the influence of NMDA receptor agonist (N-methyl-d-aspartate) and antagonist (D-AP7) on amnesia induced by a β-carboline alkaloid, harmane. Methodology: Animals implanted with bilateral cannulae at the CA1 regions of the dorsal hippocampus and microinjected with glutamatergic drugs. One-trial step-down was used to assess memory acquisition and then, the hole-board method to assess exploratory behaviors in adult male NMRI mice. Results: The results revealed that pre-training intra-CA1 administration of NMDA (0.5 ng/mouse) and D-AP7 (0.25 and 0.5 ng/mouse) improved and impaired memory acquisition, respectively. Also, pre-training intra-peritoneal (i.p.) administration of harmane (12 mg/kg) decreased memory acquisition. Furthermore, pre-training intra-CA1 injection of sub-threshold dose of NMDA (0.02 ng/mouse) reversed, while non-significant dose of D-AP7 (0.125 ng/mouse) did not change impairment of memory acquisition induced by harmane (12 mg/kg, i.p.). Conclusion: In addition, all above doses of drugs did not alter locomotor activity. These results suggest that the CA1 NMDA receptors are involved in harmane-induced amnesia.

Laparoendoscopic single-site nephrectomy and standard laparoscopic partial nephrectomy: A comparison of clinical eeficacy / 第二军医大学学报

Objective To compare the clinical outcomes of laparoendoscopic single-site (LESS) and traditional standard laparoscopy partial nephrectomy surgery, so as to investigate the characteristics, safety, feasibility, advantages and disadvantages of LESS nephrectomy surgery. Methods The same surgical group completed 12 cases of LESS partial nephrectomy from August 2009 to February 2015; 22 cases who received standard laparoendoscopic partial nephrectomy in the same period served as controls; two groups had similar tumor size and DAP scores. The clinical data were retrospectively analyzed to compare the clinical efficacies of the two groups. Results All procedures were completed successfully without conversion to open cases in the two groups. The body mass index, tumor size or DAP scores were not statistically different between the both groups (P>0. 05). The bowel recovery time, pain score, and cutouts satisfaction scores of LESS group were significantly different between the two groups ([1. 50 ± 0. 71] d vs [2. 45±± 0. 96] d, 3. 5 ± 1. 2 vs 4. 3 ± 0. 9, and 8. 1 ± 1. 8 vs 2 ± 2. 1, P0. 05). The renal glomerular filtration rate (GFR) was measured with99mTc-DTPA before and 1 month after the operation, and the GFR of LESS and standard laparoendoscopic groups had a decrease of (3. 7 ± 8. 6) mL/min and (8. 9 ± 10. 1) mL/min, respectively, showing no significant difference (P>0. 05). Conclusion The LESS nephrectomy is safe and effective, which has a similar outcome with standard laparoendoscopic partial nephrectomy when the tumor is small and in a good position.

A Case of Nasu-Hakola Disease without Fractures or Consanguinity Diagnosed Using Exome Sequencing and Treated with Sodium Valproate

Nasu-Hakola disease (NHD) is a rare autosomal recessive neuropsychiatric disorder characterized by bone cysts, fractures, and cognitive impairment. Two genes are responsible for the development of NHD; TYROBP and TREM2. Although it presents with typical signs and symptoms, diagnosing this disease remains difficult. This case report describes a male with NHD with no family or past history of bone fractures who was diagnosed using exome sequencing. His frontal lobe psychiatric symptoms recovered partially following treatment with sodium valproate, but not with an antipsychotic.

Efecto sedante del extracto alcóholico de hojas y flores de Melissa officinalis "Toronjil" más Matricaria chamomilla "Manzanilla" sobre la ansiedad inducida en ratones albinos / Sedative effect of the alcoholic extract of leaves and flowers of Melissa officinalis "Toronjil" plus Matricaria chamomilla "Chamomile" on anxiety induced in albino mice

Introducción: Ansiedad enfermedad del milenio, el cual requiere tratamiento para evitar trastorno mayores. Objetivo: Determinar efecto sedante de Melissa officinalis "Toronjil" más Matricaria chamomilla "Manzanilla" sobre ansiedad inducida en ratones albinos. Diseño: Experimental. Lugar: Facultad de Medicina y Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Material Biológico: ratones, ratas albinos. Intervenciones: Treintaises ratones fueron inducidos a hiperactividad y/o cambios conductuales por NMDA, considerándose grupos 1) SSF 10 mL/kg; 2) NMDA 75 mg/kg; 3, 4, 5) NMDA + Extracto 1, 2, 4 g/Kg correspondientemente, y 6) NMDA + DAP-7 antagónico del NMDA 0.33 nmol/kg; observándose hiperactividad, cambios conductuales, periodo latencia en minutos y porcentaje de protección. Se comparó el efecto sedante con diazepam en 30 ratones: 1) SSF 10 mL/kg; 2) diazepam 50 mg/kg; 3, 4, y 5) Extracto 1, 2, 4 g/kg respectivamente, midiéndose tiempo de dormir (minutos). Se realizó el estudio de estudio de toxicidad crónica a 60 días en 20 ratas divididos en 4 grupos: 1, 2 blanco control 3, 4, (extracto alcohólico) a dosis de 4 g/Kg. por vía oral, se evaluó los niveles tóxicos determinando a nivel, Bioquímicos: urea, colesterol, transaminasas, lipoproteína HDL e histología. Resultados: Los compuestos fenólicos y terpenoídes estuvieron en mayor cantidad en el extracto alcohólico; el 100% (p<0.05) de ratones mostró efecto sedante; los hallazgos hematológicos, bioquímicos se encontraron dentro de los límites aceptados; e histopatológicamente no hubo evidencia de cambios morfológicos. Conclusiones: El extracto alcohólico de hojas y flores de Melissa officinalis "Toronjil" más Matricaria chamomilla "Manzanilla" es sedante en ratones, y sin toxicidad en ratas.

Doença Arterial Periférica (DAP): abordagem inicial / Peripheral Artery Disease (PAD): initial approach

Doenças crônicas acentuadas por um inadequado estilo de vida, como a DAP, ganham espaço nos atendimentos primários com o aumento da expectativa de vida e o envelhecimento da população. Este artigo de revisão visa à abordagem inicial, no diagnóstico, na conduta e em um possível encaminhamento para centros especializados.

Chronic diseases marked by inadequate lifestyle, such as DAP, earns space in primary care with the increasing of life expectancy and the aging of population. This review article aims the initial approach, diagnosis, conduct and a possible referral to specialized centers.

Estructura y diversidad de la vegetación del Parque Nacional Natural de la Isla Gorgona, Colombia / Structure and diversity of the vegetation of the Gorgona Island National Natural Park, Colombia

Gorgona is a continental island in the Pacific of Colombia. We studied the structure and diversity of its vegetation with 16 Gentry transects (total area 0.16ha) in nine sites on the island. With these data, we calculate Importance Value Index (IVI), distribution of individuals according to their height and diameter at breast height (DBH), cumulative species curves, and species diversity. The vegetation is mainly secondary forest, with remnants of primary forest on the hills. By density, frequency, and coverage, the most important family is Rubiaceae, with 0.275 IVF. The species with the highest IVI (0.157) is Lacistema aggregatum (Lacistemataceae). In height and diameter classes (DBH), individuals have an inverted “J” distribution. The plant community has a Shannon-Wiener diversity index of 3.98. The cumulative species curve of the plant community does not level off even after more than ten transects. Transects were grouped in the similarity analysis but not strongly. There were species richness differences among transects. Rev. Biol. Trop. 62 (Suppl. 1): 13-26. Epub 2014 February 01.

En este trabajo, se estudió la estructura y diversidad de la vegetación en isla Gorgona, a través de 16 transectos de Gentry (área total de 0.16ha), distribuidos en nueve sitios de la isla. Se calculó el índice de Valor de Importancia para la especie y la familia (IVI e IVF respectivamente), la distribución de los individuos en función de su altura y diámetro a la altura del pecho (DAP ), las curvas de especies acumuladas , y la diversidad de especies. La vegetación de isla Gorgona estuvo conformada principalmente por bosque secundario, con restos de bosque primario en las partes altas de los cerros. De acuerdo con la densidad, la frecuencia, y la cobertura, la familia más importante fue Rubiaceae (IVF=0.275), mientras que la especie con el IVI más alto fue Lacistema aggregatum (Lacistemataceae, IVI=0.157). De acuerdo con la altura y clases de diámetro (DAP), la distribución de los individuos describió una forma de “J” invertida, con un valor de diversidad (H’) calculado de 3.98. Incluso después de más de diez transectos de muestreo, la curva de acumulación de especies no se niveló. Con base en el análisis de similitud, se estableció que los transectos estuvieron agrupados en zonas, de las cuales cuatro tuvieron un soporte estadístico, siendo incluso la riqueza de especies diferente entre los transectos.

Correlation between vasoactive intestinal peptide and expression of NKG2D, adaptor protein 10 and extracellular signal-regulated kinase in inflammatory cells of gastric adenocarcinoma / 中华消化杂志

Objective To investigate the expression of NKG2D and related signaling molecules adaptor protein(DAP10) and extracellular signal-regulated kinase(ERK) in the inflammatory cells of gastric adenocarcinoma and para-carcinoma tissues,the effects of vasoactive intestinal peptide(VIP) on the expression of NKG2D,DAP10 and ERK in natural killer(NK) cells of healthy volunteers.Methods The expression of NKG2D,DAP10 and ERK in the inflammatory cells of 36 gastric adenocarcinoma and para-carcinoma tissues were detected by immunohistochemistry.The peripheral blood NK cells of healthy volunteers were isolated and purified with CDC method.The expressions of NKG2D,DAP10 and ERK in NK cells were determined by immunocytochemistry and reverse transcription-polymerase chain reaction(RT-PCR) methods before and after VIP intervention.The ttest was used for comparison between two groups and rank transformation nonparametric test (Kruskal-Wallis H test) for count data analysis.Results The expression levels of NKG2D,DAP10 and ERK in the inflammatory cells of gastric adenocarcinoma were significantly lower than those of para-carcinoma tissues (H=30.640,24.910,20.320,all P＜0.01).The expression levels of NKG2D,DAP10 and ERK were much lower in poorly differentiated gastric adenocarcinoma (H =4.049,11.830,8.118),at stage Ⅲ-Ⅳ (H=4.275,11.560,7.686),and the expression levels of NKG2D,ERK were lower with lymph nodes metastasis (H=6.513,6.064,all P＜0.05).The expression levels of NKG2D,DAP10 and ERK in gastric adenocarcinoma tissues and inflammatory cells were not correlated with gender,age and the location of lesions (all P＞0.05).The expression levels of NKG2D,DAP10 and ERK in NK cells was decreased after VIP intervention (protein:H=12.438,4.798,13.745,mRNA:F=337.640,638.579,1055.015,all P＜0.05).Conclusion Gastric adenocarcinoma may downregulate the expression of NKG2D,DAP10 and ERK in NK cells through VIP secretion,which may be involved in the immune escape of gastric cancer.

Death-associated protein kinase is underexpressed in high-grade oral squamous cell carcinoma / La expresión de la proteína quinasa asociada a muerte celular (DAP quinasa) está reducida en carcinoma de células escamosas oral de alto grado

An immunohistochemical analysis of 40 cases of oral squamous cell carcinoma was performed to evaluate the relationship between the expression pattern of death-associated protein kinase (DAPk) positive cells with the histological malignancy grading of these lesions. According to our results, eleven cases (27.5 percent) were high-grade malignancy tumours and 29 (72.5 percent) were low-grade ones. We found that 92.86 percent of the low-grade tumours were positive to anti-DAP kinase antibody whereas only 7.14 percent of the high-grade tumours presented positivity, and this difference was statistically significant (p<0.01). Sixteen (55.2 percent) of the low-grade carcinomas exhibited moderate immunoreactivity whereas ten cases (34.5 percent) showed weak staining and three cases (10.3 percent) were negative tumours. Immunostaining was lacking in nine (81.8 percent) of the high-grade carcinomas and "weak" in the two (18.2 percent) remaining cases. Thus, DAPk expression is significantly decreased in high-grade oral carcinomas, and evidences indicate that it might be related to the severity of cytological atypia.

Fue realizado un análisis inmunohistoquímico de 40 casos de carcinoma oral de células escamosas para evaluar la relación entre el patrón de expresión de la proteína quinasa (DAPK) asociada a la muerte celular y la clasificación histológica de malignidad de estas lesiones. Según nuestros resultados, 11 casos (27,5 por ciento) eran tumores de alto grado de malignidad y 29 (72,5 por ciento) de bajo grado. Se encontró que 92,86 por ciento de los tumores de bajo grado de malignidad fueron eran positivos para anticuerpos anti-DAP-quinasa, mientras que sólo 7,14 por ciento de los tumores de alto grado presentaron positividad, esta diferencia fue estadísticamente significativa (p <0,01). En 16 casos (55,2 por ciento) los carcinomas de bajo grado de malignidad mostraron inmunorreactividad moderada mientras que 10 casos (34,5 por ciento) mostraron una tinción débil y 3 casos (10,3 por ciento) fueron negativos. La inmunotinción estuvo ausente en 9 (81,8 por ciento) de los carcinomas de alto grado y "débil" grado de malignidad en los 2 (18,2 por ciento) casos restantes. Así, la expresión DAPK se redujo significativamente en los carcinomas orales de alto grado y las evidencias indican que podría estar relacionado con la severidad de la atipia citológica.

Aberrant Promoter Methylation of Death-Associated Protein Kinase in Serum DNA from Lung Cancer Patients / 결핵

BACKGROUND: Promoter methylation of tumor suppressor genes is one of the key epigenetic changes in many human cancers. The aim of this study was to evaluate the promoter methylation status of the Death-associated protein(DAP) kinase gene, which played an important role in lung cancer, in the serum DNA of primary lung cancer patients. METHODS: This study investigated the aberrant methylation of DAP kinase in the serum of 65 primary lung cancer patients by methylation-specific PCR (MSP). RESULTS: Methylation in the serum was detected in 29 of 65(44.6%) for DAP kinase. There was no statistical association between methylation of DAP kinase and age, smoking history, histologic type, or stage. Methylation of DAP kinase was found more frequently in men (p=0.044). CONCLUSIONS: This study suggests that the aberrant methylation of the DAP kinase promoter is readily detectable in the serum DNA of lung cancer patients using MSP analysis.

Método para determinar o diâmetro máximo de corte em florestas tropicais utilizando-se o gráfico "Uplot-Dap"

This research presents a method for determination of the maximum diameter of cut in tropical forests being used the denominated graphic construction of "Uplot-Dap". The main objective consisted on detecting the existence and location of a possible maximum diameter in function of the biological balance of the species in a climax forest. To test the graphic method they were used 28320 DBH (cm) outside bark coming from 15 blocks of 96 hectares is of the National Forest of Tapajós, Belterra, Pará, Brazil. The method that uses technique of visual analysis of data as a way of interpretation of the dispersion of the diameters, was shown quite flexible and essentially practical. The graphs were built considering a width of diameter class of 10 cm adopted as reference value. Through this method it was possible to fasten the DBH=95 cm as an indicator of the maximum diameter of exploitability to be adopted in the 1st intervention as reference value in the cut operations for this forest type. Mean while this point can vary from forest to forest and its clearness depends on the forest type and its structural condition. The largest contribution of the graphic method consisted on respecting the properties of the diameter classes in natural forests that for biological reasons or sucessional estate, express its diameter distribution as a function of rare, null or probabilistics events. The efficiency of the method bases on its capacity of controlling the variation of the data so that the analysis are more consistent with the physical and biological factors that characterize the dynamics of natural forests.

A presente pesquisa apresenta um método de determinação do diâmetro máximo de corte para florestas tropicais utilizando-se a construção gráfica denominada de "Uplot-Dap". O objetivo da pesquisa foi detectar a existência e locação de um possível diâmetro máximo em função do equilíbrio biológico das espécies em uma floresta clímax, utilizando-se o método gráfico "Uplot-Dap". Para testar o método gráfico foram utilizados 28320 DAP com casca de árvores procedentes de 15 blocos de 96 ha da Floresta Nacional do Tapajós, Belterra, Pará. Os gráficos foram construídos considerando uma amplitude de classe diamétrica de 10 cm adotada como valor de referência. O método "Uplot-Dap" que utiliza técnica de análise visual de dados como forma de interpretação da dispersão dos diâmetros, mostrou-se bastante flexível e essencialmente prático. Através do método foi possível fixar o DAP=95 cm como um indicador do diâmetro máximo de explorabilidade a ser adotado na primeira intervenção como valor de referência nas operações de corte para esse tipo de floresta. Todavia, esse ponto pode variar de floresta para floresta e sua nitidez depende do tipo de floresta e suas condições estruturais. A maior contribuição do método gráfico consistiu em respeitar as propriedades das classes diamétricas em florestas naturais que por razões biológicas ou estágio sucessional, expressem a sua distribuição diamétrica como uma função de eventos raros, nulos ou probabilísticos. A eficiência do método baseia-se na sua capacidade de controlar a variação dos dados de modo que as análises sejam mais consistentes com os fatores físicos e biológicos que caracterizam a dinâmica de florestas naturais.