Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BSF) of T. b. brucei: (i) fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hexokinase, phosphofructokinase, glucose-6-phosphate isomerase, phosphoglycerate kinase, triosephosphate isomerase (glycolytic enzymes) and glycerol-3-phosphate dehydrogenase (a glycolysis-auxiliary enzyme) in glycosomes, (ii) enolase, phosphoglycerate mutase, pyruvate kinase (glycolytic enzymes) and a GAPDH isoenzyme in the cytosol, (iii) malate dehydrogenase in cytosol and (iv) glucose-6-phosphate dehydrogenase in both glycosomes and the cytosol. Specific enzymatic activities also suggest that T. evansi is alike to the BSF of T. b. brucei in glycolytic flux, which is much faster than the pentose phosphate pathway flux, and in the involvement of cytosolic GAPDH in the NAD+/NADH balance. These similarities were expected based on the close phylogenetic relationship of both parasites.

Examining relationship between environmental gradients and Lesser Himalyan forest vegetation of Nikyal valley, Azad Jammu and Kashmir using ordination analysis

OBJECTIVE@#To report the relationship of vegetation structure and environmental gradient and physiochemical properties of soil at Nikyal valley, Azad Jammu and Kashmir .@*METHODS@#A survey of natural vegetation and soil of Nikyal valley was undertaken. Phytosociological survey was conducted by using Braun-Blanquet's approach. The study also investigated the vegetation structure and its relationship with altitude and edaphic factors. The floristic data was analyzed by cluster anlaysis, detrended correspondence analysis and canonical correspondence analysis using CANACOO 5.0.@*RESULTS@#A total of 110 plant species and 13 stands were merged into five major associations as dema rcated by cluster analysis and detrended correspondence analysis. The associations were Olea-Pinus-Themeda association, Myrsine-Rhus-Quercus association, Quercus-Rubus-Pinus association and Quercus association. Soil organic matter, saturation, pH and altitude play the major role in distribution of species.@*CONCLUSIONS@#The variation in vegetation structure is controlled by the altitudinal gradient and physiochemical properties of soil. These results indicate a deteriorated forest structure and reduced regeneration pattern, demanding immediate attention of forest management authorities.

Examining relationship between environmental gradients and Lesser Himalyan forest vegetation of Nikyal valley, Azad Jammu and Kashmir using ordination analysis

Objective: To report the relationship of vegetation structure and environmental gradient and physiochemical properties of soil at Nikyal valley, Azad Jammu and Kashmir Methods: A survey of natural vegetation and soil of Nikyal valley was undertaken. Phytosociological survey was conducted by using Braun-Blanquet's approach. The study also investigated the vegetation structure and its relationship with altitude and edaphic factors. The floristic data was analyzed by cluster anlaysis, detrended correspondence analysis and canonical correspondence analysis using CANACOO 5.0. Results: A total of 110 plant species and 13 stands were merged into five major associations as dema rcated by cluster analysis and detrended correspondence analysis. The associations were Olea- Pinus- Themeda association, Myrsine- Rhus- Quercus association, Quercus- Rubus- Pinus association and Quercus association. Soil organic matter, saturation, pH and altitude play the major role in distribution of species. Conclusions: The variation in vegetation structure is controlled by the altitudinal gradient and physiochemical properties of soil. These results indicate a deteriorated forest structure and reduced regeneration pattern, demanding immediate attention of forest management authorities.

DCA on medicinal materials of three species of Isodon Kudo based on their UV spectra / 中草药

Object To observe the differences of Isodon amethystoides (Benth.) C. Y. Wu et Hsuan, I. nervosa (Hemsl) Kudo and I. macrocalyx (Dunn) Kudo from different regions in their UV spectra, to make the applicability of the method of Detrended Correspondence Analysis (DCA) clear in the comparison of Chinese medicinal materials based on the UV spectra. Methods The UV spectra of the 15 samples of I. amethystoides, I. nervosa and I. macrocalyx from different regions and organs were obtained, based on the indices of wavenumber absorbance, the differences of 15 UV spectra were compared by DCA. Results(1) the UV spectra of I. amthystoides from different regions were very different; (2) the samples of I. macrocalyx (or I. nervosa) from the same region were rather similar in their UV spectra; (3) there were resemblances among I. nervosa, I. macrocalyx and I. amethystoides in their UV spectra.; (4) the difference of UV spectra between the stems and leaves were obvious. Conclusion The above results indicate: (1) the medicinal effects of I. amethystoides from different regions may be very different; (2) the stems and leaves of I. amethystoides are of different medical effects; (3) I. macrocalyx and I. nervosa could be considered as the substitutes of I. amethystoides. It is practical to apply DCA on the basis of UV spectra to compare the chemical differences of plant samples.