RESUMO
Objective: To study the chemical constituents in Qili Qiangxin Capsule (QQC) and to clear its material basis for efficacy. Methods: A rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS) method was established to identify the diterpenoid alkaloids in the active fraction of QQC. Using target compound screening method, the structures of monitored chemical constituents were identified by retention time, exact relative molecular mass, and cleavage fragments of MS/MS. Results: Twenty diterpenoid alkaloids were identified using XIC Manager, including 6 diester-diterpenoid alkaloids and 14 monoester-diterpenoid alkaloids. Conclusion: This method is simple and rapid for the identification of the constituents in QQC and the results are useful for the quality control of QQC.
RESUMO
OBJECTIVE: To research the content of alkaloids and polysaccharides in different varieties of Aconitum carmichaeli. METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively. RESULTS: In different varieties, the differences of total alkaloids and diester diterpenoid alkaloids were significant. However, the difference of polysaccharides wasn’t significant. The content of total alkaloids and diester diterpenoid alkaloids in processed A. carmichaeli reduced significantly while the content of polysaccharides increased slightly. CONCLUSION: The results of study could provide a scientific basis for rational breeding of A. carmichaeli.