Four changes of plasma D- dimer and coagulation in pregnant women at different stages of pregnancy and their value of test / 中国生化药物杂志

Objective To observe and analyze the four changes of plasma D- dimer and coagulation in pregnant women at different stages of pregnancy, and to explore the value of the test. Methods The subjects of this study were 120 pregnant women who underwent routine physical examination in our hospital, according to different stages of pregnancy (early pregnancy, middle pregnancy, late pregnancy, and before delivery) Four D- dimer and four blood coagulation tests were performed in pregnant women, and the results were observed and compared. Results During the pregnancy, D- dimer has increase trend (P<0.05); early in pregnancy, pregnancy and late pregnancy, PT has shortened(P<0.05); pregnancy, APTT has shortened (P<0.05), at the end of pregnancy and parturient period, showing a significant shortening (P<0.05); phenomenon in early pregnancy to mid pregnancy stage, FIB increased significantly (P<0.05), and at the end of pregnancy to perinatal period, with a sharp increase (P<0.05). Conclusion It is of great significance to perform dynamic monitoring of of plasma D- dimer and coagulation four indices in pregnant women during pregnancy.

Effect of ventilation with low tidal volume and positive end-expiratory pressure in different periods on postoperative pulmonary function and short-term prognosis in elderly patients with abdominal surgery / 临床麻醉学杂志

Objective To observe the effects of ventilation with low tidal volume and positive end-expiratory pressure (PEEP)in different periods on the postoperative pulmonary function and short-term prognosis in aged patients undergoing abdominal surgery.Methods Sixty aged patients undergoing selective open abdominal surgery scheduled for general anesthesia,21 males and 39 fe-males,were randomized into 3 groups (n =20).Patients in group A received PEEP 1 h after the be-ginning of surgery;patients in group B received PEEP 1 h before tracheal extubation;patients in group C received PEEP intraoperatively.The secretion score in preoperative,postoperative 24 h and 72 h respectively,and the arterial blood gas analysis indexes (PaCO 2 ,PaO 2 ,A-aDO 2 ,PaO 2/FiO 2 calculation)in postoperative 1 h and 24 h were recorded.Results Compared with preoperative,in postoperative 1 h,PaCO 2 increased obviously in all groups,PaO 2 decreased in group B,A-aDO 2 in-creased in group A (P <0.05);in postoperative 24 h,PaCO 2 was significantly increased in group B and C,PaO 2/FiO 2 decreased in group B (P <0.05).Compared with postoperative 1 h,in postopera-tive 24 h,PaCO 2 and A-aDO 2 decreased obviously in group A (P <0.05).There were no differences in postoperative secretions score in between the 3 groups.Conclusion Low tidal volume combined short-range PEEP in different periods of surgery may improve postoperative pulmonary oxygenation. But they had no obvious help with postoperative pulmonary complications.

Effects of Bauhinia championii flavones on adjusting autophagy against myocardial ischemia/reperfusion injury / 中国药理学通报

Aim To study the effects of Bauhinia championii flavones ( BCF ) on adjusting autophagy a-gainst myocardial ischemia reperfusion injury. Meth-ods 120 SD rats were randomly divided into control, model, 3-MA (15 mg·kg-1 ) and BCF (10, 20 mg ·kg-1) groups (n=8). 30min. ischemia, ischemia/reperfusion 1h. or 3h, while myocardial ischemia reperfusion model was established by ligating left ante-rior descending coronary artery. The contents of crea-tine kinase-MB ( CK-MB) , inducible nitric oxide syn-thase ( iNOS ) and total antioxidative capacity ( T-AOC ) in serum were determined according to the ultra-violet spectrophotometer colorimetric method. The pro-tein expression of LC3-Ⅱ, Beclin-1 and mTOR was observed by Western blot. Results Compared with model group, BCF dose-dependently improved T-AOC, decreased CK-MB and iNOS ( P < 0. 05 ) . Meanwhile, BCF down-regulated the protein expression of LC3-Ⅱand Beclin-1 , and up-regulated mTOR pro-tein expression ( P < 0. 05 ) . Compared with 3-MA group, BCF reduced the contents of CK-MB and iN-OS, increased the level of T-AOC. It raised LC3-Ⅱand Beclin-1 protein expression at 30min. of ischemia, and then declined the protein expression of LC3-Ⅱand Beclin-1 . BCF descended the protein expression of mTOR all over the time ( P <0. 05 ) . Conclusion Autophagy occurs in ischemia then improves in ische-mia/reperfusion period. BCF could promote the occur-ring of autophagy at 30min. of ischemia in the short term and inhibit the excessive expression of autophagy with the increase of time in reperfusion period. BCF pretreatment reduce myocardial ischemia/reperfusion injury may by promoting the occurrence of autophagy during ischemia period and inhibit them in reperfusion.

Study of Exercise-induced Fatigue Rat Model Established by Different Periodical Swimming Training Based on Evaluation of Liver Function / 广州中医药大学学报

Objective To study the influence of swimming training for different periods on rat liver function, and to evaluate the quality of exercise-induced fatigue rat model established by swimming training for different periods. Methods Forty SD rats were randomly divided into 5 groups, namely blank group, and 7-, 14-, 21-and 28-day exercise groups, 8 rats in each group. Rats in the blank group were fed with normal diet, but were not given exercise training. The other groups were given exercise training for 7, 14, 21 and 28 days respectively, two times a day, 15 minutes every time and with 10-minute resting interval between the two times. At the last day of the experiment, all rats were executed by neck dislocation for the sampling of blood and liver tissues, and then the serum contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. And the contents of total anti-oxidization capacity (T-AOC), malondialdehyde (MDA), lactic acid (LA), lactate dehydrogenase (LDH) and glucose (Glu) in liver tissues were also examined. Results Compared with the blank group, the contents of ALT and AST were increased in 7-, 14-, 21-, and 28-day exercise groups, especially in 21-and 28-day exercise groups (P<0.01) . The contents of T-AOC and MDA were increased in 7-, 14-, 21-, and 28-day exercise groups (P<0.01), the effects on T-AOC in the 21-and 28-day exercise groups and on MDA in the 28-day exercise group being more obvious (P<0.05 or P<0.01) . The LA content and LDH activity were increased and the glycogen was decreased in the liver tissues of 7-, 14-, 21-, and 28-day exercise groups, and the increase of LA content in 21- and 28-day exercise groups and that of LDH activity in 28-day exercise groups were more significant ( P<0.05 or P<0.01). The hepatic glycogen content in 14-, 21-, and 28-day exercise groups were lower than that in the other groups (P<0.01). Conclusion With hepatic function injury in exercise-induced fatigue as the criteria, the exercise-induced fatigue rat model has been successfully established in this study, and the model established by swimming training for 21 and 28 days is more steady.

Clinical application of diffusion tensor imaging in acute stroke / 中国综合临床

Objective To reveal the transmiting discipline of parameters and define therapy time window of ischemia penumbra by continuous detection of diffusion tensor imaging(DTI) o Methods 35 patients with acute cerebral infarction in different phases were imaged with conventional DWI and DTI.To measure DCavg,FA,RA,1-VR,ADC in different regions of interest (ROI).Results Change in DCavg had tendency which appear reduced obviously,elevated,renormalized,increased.FA/RA/1-VR of lesions elevated in hyperacute stroke and unrecovered declined acute to sub-acute stroke.Difference of DCavg,FA,RA,1-VR between central and periphral parts of lesions were significant in hyperacute and acute stroke.ADC of lesions were significant in hyperacute and acute stroke.Difference of FA between central and peripheral parts of lesions were significant in acute stroke(P ＜ 0.01).Difference of ADC,DCavg between central and peripheral parts of lesions were significant in acute and subacute stroke(P ＜ 0.01).Conclusion It had characteristic discipline in changes of DCavg,FA,RA,1-VR,ADC,The peripheral parts of lesions in hyperacute and acute stroke maybe ischemia penumbra,DTI has important practical value for clinical.