UGT1A1 gene mutations in Chinese Dong neonates in Sanjiang, Guangxi / 中国当代儿科杂志

OBJECTIVES@#To study the characteristics of UGT1A1 gene mutations in Dong neonates in Sanjiang County of Liuzhou and its association with the pathogenesis of hyperbilirubinemia in Dong neonates.@*METHODS@#A prospective analysis was performed on 84 neonates who were diagnosed with unexplained hyperbilirubinemia in the Department of Neonatology, Sanjiang County People's Hospital, from January 2021 to January 2022. Sixty healthy neonates born during the same period were enrolled as the control group. Peripheral blood genomic DNA was extracted for both groups, and UGT1A1 exon 1 was amplified by PCR and sequenced.@*RESULTS@#In the case group, 33 neonates were found to have G71R missense mutation, with a mutation rate of 39%. The case group had a significantly higher frequency of A allele than the healthy control group (21% vs 10%, P<0.05). The risk of hyperbilirubinemia in Dong neonates carrying G71R missense mutation was 2.588 times as high as that in healthy neonates carrying wild-type UGT1A1 gene (P<0.05). Hardy-Weinberg equilibrium testing showed that the UGT1A1 G71R locus was in genetic equilibrium in both groups (P>0.05).@*CONCLUSIONS@#UGT1A1 G71R mutation is a high-frequency gene mutation type in Dong neonates in Sanjiang County, and G71R missense mutation is associated with hyperbilirubinemia in Dong neonates.

Chemical constituents from an ethnic drug Dicranopteris pedata / 中国药学杂志

OBJECTIVE: To study the chemical constituents of the ethnic drug Dicranopteris pedata. METHODS: The compounds were isolated and purified by comprehensive application of silica gel and Sephadex LH-20 chromatography, and their structures were identified on the basis of comprehensive analysis of their physical and chemical properties, NMR data and references. RESULTS: Fourteen compounds were isolated and elucidated as quercitrin(1), kaempferol 3-O-α-L-rhamnoside(2), rutin(3), hyperoside(4), kaempferol(5), quercetin(6),2-hydroxy-4-methoxy-2',3'-benzochalcone(7),stigmasterol(8), cycloastragenol(9), shikimic acid(10), protocatechuic acid(ll), gallic acid(12),β-sitosterol(13), and daucosterol(14). CONCLUSION: Compound 1-14 are for the first time isolated from D. pedata.