RESUMO
Objective: To explore the effects of drynaria total flavonoids on serum estrogen (E2) level, OPG/RANKL balance and bone microarchitecture of mandible in a rat model of bone loss induced by ovariectomy. Methods: We randomly divided 40 three-month-old female Sprague-Dawley rats into 5 groups (n=8 per group): sham-operation group (Sham), ovariectomized group (OVX), low-dose drynaria total flavonoids group (GB-L), high-dose drynaria total flavonoids group (GB-H) and estradiol valerate group (EV). The mandibular osteoporosis model was established by removing the rats' ovaries. After 12 weeks the rats were killed, and the serum estrogen levels were measured. OPG and RANKL were detected using ELISA method. Therapeutic effect of drynaria total flavonoids was observed by pathomorphology and histomorphometry of the mandibular molar sections. Results: Compared with those in the sham group, the mandible of rats in OVX group was loosely changed, the level of E2 decreased significantly (P<0.001), and OPG/RANKL decreased significantly (P<0.001). Compared with OVX group, the mandible microstructure of rats in EV group got a good repair, while the serum E2 level and OPG/RANKL increased significantly (both P<0.001). Compared with OVX group, the percentage of trabecular bone (%Tb.Ar) and OPG/RANKL increased in all the GB groups (P<0.001, P=0.029), (P<0.001, P=0.007), while GB-H group also had increased thickness of trabecular bone (Tb.Th) and serum OPG levels (P=0.019, P<0.001), reduced trabecular spacing (Tb.Sp)(P=0.001), but no effect on serum E2 level (P=0.432, P=0.459). Conclusion: Drynaria total flavonoids can inhibit the destruction of the mandibular trabecular structure, thus slowing down the mandibular resorption process. However, they cannot improve E2 concentration. The mechanism is realized by adjusting the balance of OPG/RANKL.
RESUMO
Objective To investigate the protective effect of total flavonoids of drynaria on hemorrhagic shock reperfusion renal injury.Methods The 30 Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, experimental group (hemorrhagic shock group) and drynaria fortunei group, 10 in each group. In drynaria fortunei group and experiment group, the blood from the femoral artery was with drawn until the mean arterial pressure reached 40 mmHg in 90 min, and then the withdrawn blood transfusion were reinfused and blood pressure recovered to the base value of 90%. The rats of drynaria fortunei group were given intraperitoneal injection of Drynaria total flavonoid 300 mg/kg, and experimental group and sham operation group were injected with 3 ml physiological saline. Blood creatinine (SCr) levels, the renal cell apoptosis index, the expression of Bcl-2 and mRNA Bax in kidney were detected. Results Compared with the experimental group, serum SCr (78.36 ± 5.31 mmol/Lvs. 151.43 ± 11.8 mmol/L) of drynaria fortunei group decreased significantly (P<0.01); the renal cell apoptosis (19.56% ± 4.37%vs. 14.31% ± 2.83%), the expression level of Osteopractic rats of Bax group mRNA (1.24 ± 0.05vs. 0.91 ± 0.07) of drynaria fortunei group decreased significantly; and the Bcl-2 mRNA (0.65 ± 0.05vs. 1.38 ± 0.07) and the expression level and Bcl-2/bax ratio (0.52 ± 0.06vs. 1.51 ± 0.08) of drynaria fortunei group increased significantly (P<0.01).Conclusions The drynaria total flavonoids on rats with hemorrhagic shock and resuscitation of renal injury has a protective effect, and its mechanism may improve the expression of Bcl-2 and mRNA, increase the ratio of bcl-2/bax, and inhibit or block the apoptosis of renal tissue.