RESUMO
Objective: To optimize the separation and purification of the total poly phenol from Dryopteris crassirhizoma with anion- exchange resin. Methods: The rates of elution and absorption, contents of dryocrassin ABBA, and total ploy phenol were used as makers to optimize the purification conditions of total ploy phenol. Results: The extracting solution of D. crassirhizoma passed through 201×7 hydrogen-oxygen the anion-exchange resin column (column diameter-column height, 1:7) at the rate of 6 BV/h in reverse direction, then the resin column was flushed with water at the rate of 6BV/h to pH value 6-7 in forward direction. At last, the column was eluted at the rate of 6 BV/h to obtain ploy phenol with 9BV 3% salt water in 60% alcohol. The elution ratio of dryocrassin ABBA is 90.1%, and the total ploy phenol is 91.1%. The content of dryocrassin ABBA is 29.4% and the content of the total ploy phenol is 49.2%. Conclusion: The separation and purification of the total ploy phenol from D. crassirhizoma with 201 × 7 hydrogen-oxygen anion-exchange resin can achieve the satisfactory results which have wide application prospects.