Novel carbon dots derived from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum and their hemostatic effect / 中草药

Objective: A new type of nanoparticles-carbon dots was identified from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum (DCC) and investigated their hemostatic effect. Methods DCC was boiled, filtered, and dialyzed to separate and obtain a novel type of carbon dots named DCC-CDs. These DCC-CDs were characterized using transmission electron microscopy (TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet-visible, X-ray photoelectron spectroscopy, and HPLC. Then, the anti-hemorrhagic effects were evaluated by severed tails ad liver bleeding tests and the related hemostatic mechanisms of the obtained DCC-CDs were assessed by detecting blood coagulation and PLT quantity to discuss the hemostasis mechanism. Results The DCC-CDs separated from DCC were spherical, monodisperse, which had a narrow size distribution between 1 and 7 nm. In the animal experiment, DCC-CDs were revealed excellent hemostatic bioactivity, and significant decreased in the tail and liver bleeding time (P < 0.01). Additionally, the rats showed a profound increasing in fibrinogen (FIB) and platelets (PLT) after DCC-CDs treatment (P < 0.05). Conclusion These results demonstrated an explicit hemostatic effect of DCC-CDs, which offers new avenues for research into DCC to treat hemorrhagic diseases and a new mindset for the exploration of the active ingredients of traditional Chinese medicine.

Identification of Dryopteridis Crassirhizomatis Rhizoma based on psbA-trnH barcode / 中国中药杂志

To identify origin of the medicinal materials Dryopteridis Crassirhizomatis Rhizoma by using the psbA-trnH sequence, the polymerase chain reaction (PCR) amplification and product sequencing of the experimental samples were performed. In order to expand the scope of the study, the psbA-trnH sequences of 8 genera and 3 species were downloaded from GenBank for analysis. DNAMAN 8.0 software was used to show splicing and comparison results of the peak diagrams with analysis of them, and MEGA 6.0 software was to calculate K2P genetic distances and establish clustering tree adjacent genus. The results showed that by using the psbA-trnH sequence, Dryopteridis Crassirhizomatis Rhizoma, its original plant and other easy-confused medicinal materials and plants can be distinguished with each other obviously, with the psbA-trnH sequence of Dryopteridis Crassirhizomatis Rhizoma completely consistent with that of its original plant. Consequently, it is revealed that it's feasible to identify Dryopteridis Crassirhizomatis Rhizoma and its original plant, and separate from its adulterants by means of the psbA-trnH sequence, which can provide more scientific bases for the further study of the identification of the ferny medicinal herbs.