Advance in studying early bolting of Umbelliferae medicinal plant / 中国中药杂志

The early bolting has become one of the key issues affecting the Umbelliferae herbs production. The early bolting does not only reduce herbs production but also affect the active ingredients of plants. The concept of early bolting, physiological theory, induce factors of the quality of medicine and, control technology about the early bolting of Umbelliferae are integrative reviewed in the paper. Its occurrence mechanism include: genetic factors, environmental and ecological factors, endogenous hormones, sowing, fertilizer and other production management measures. For the above occurring mechanism, the proposed prevention measures are as follows: preferably germplasm, sowing, rational fertilization, exogenous hormones, shading, reed head cut off, cut fall the leaves, reasonable planting density and other agronomic measures can reduce its bolting rate. And the early bolting problem focused for future research are mentioned, including: development of authentic ingredients cultivation technical specifications, screened early bolting hormone inhibitor, in-depth study of the mechanism of early bolting, reveal its early bolting gene and key ecological factors. The research of vernalization gene, gene regulation of flowering early bolting will solve the problem of quality and production reducing fundamentally caused by the early bolting.

Study on Physiological Mechanisms in Frozen Storage to Reduce Early Bolting of Angelica Sinensis / 世界科学技术-中医药现代化

This article was aimed to study the physiological mechanisms in frozen storage to reduce early bolting of Angelica sinensis. By analyzing the root soluble sugar, soluble protein, MDA content changes before and after -10oC processing of the Angelica sinensis seedlings of different size from different sources , the physiological mechanisms in frozen storage reducing early bolting of A . sinensis had been studied. The results indicated that the greater seedlings , the higher content of soluble sugar , lower protein content and higher MDA . There were no obvious differences on the soluble sugar , soluble protein and MDA content of the same size seedlings from different sources . After -10oC freezing , the content of soluble sugar and protein in the seedlings with the same size were decreased. Meanwhile, the content of MDA was increased. It was concluded that the content of soluble sugar, soluble protein and MDA were obviously related to the size of Angelica sinensis seedlings from the same source before and after low temperature process .

cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels

Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20 percent-60 percent, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and sequencing, 32 distinct sequences were obtained from these 26 TDFs, and 25 were found with homologous sequences in databases. Confirmation of differential expression of 13 sequences was obtained by semi-quantitative RT-PCR, their showing higher expression levels in flower buds. These homologous sequences encode transposable elements, pentatricopeptide repeat-containing proteins, DNA-binding transcription factors, zinc finger (B-box type) family proteins, NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH), amongst others.

Effect of root diameter on early bolting rate and yield in seedling of Angelica sinensis / 中草药

Objective To investigate the relationship between the root diameter of Angelica sinensis and its early bolting rate, and to decrease the bolting rate. Methods The seedling transplanting of A. sinensis with various root diameters was used to study the bolting rate and yield. Results Bolting plants can be observed in the field at June and the period from June to July is the bolting heyday. The bolting rate was the highest, up to 94.65%, in the treatment of root diameter ≥ 0.86 cm. However, it was only 1.63% in the treatment of root diameter ≤ 0.35 cm on Oct. 2nd. In the treatment of root diameter ≤ 0.35 cm, the seedling rate was only 33.62%, while in the treatment of root diameter ≥ 0.86 cm, the seedling rate was the highest, up to 95.75%, but the harvest seedling number was only 6 395 plant/hm2. In the treatment of root diameter of 0.46—0.55 cm, the harvest seedling number was 68 398 plant/hm2, which was significantly higher than other treatments. The highest yield appeared in the treatment of root diameter in 0.46—0.55 cm of transplanting seedling, the fresh and dry yields of A. sinensis were 5 717.58 kg/hm2 and 1 554.98 kg/hm2, respectively. Conclusion The seedlings with root diameter in the range of 0.46—0.65 cm should be selected to transplant in practice.