Eclipta alba Derived Phytochemicals against Jaundice

Phytochemicals otherwise known assecondary metabolites means the small organic molecules that are not essential for growth, development and reproduction but can protect from various diseases. Infact these phytochemicals are the key source of medicine. Secondary metabolites from Eclipta alba plant extract are traditionally used to cure Jaundice which is caused by Leptospira interrogans. The curative property of Eclipta alba against Leptospira interrogans was proved by molecular docking method in “Biovia Discovery Studio”. “High positive values of -CDOCKER energy and -CDOCKER interaction energy” recommended that pentadecan, heptadecane, 6,10,14-trimethyl-2-petadecanone can successfully deactivate the thioredoxin-disulfide reductase enzyme thereby disrupting the cellular function as well as the lifecycle of causative organism

Eclipta alba L. Derived Phytochemicals against Escherichia coli Causing Diarrhea

Phytochemicals from Eclipta albaL.plant extract are traditionally used to cure Diarrhea. It is caused by Escherichia coli.Molecular docking method applied using “Biovia Discovery Studio”. “High positive values of -CDOCKER energy and -CDOCKER interaction energy” suggested that Pentadecanone can effectively deactivate the alcohol dehydrogenase enzyme (protein database code 4GKV) thereby interrupting the life cycle of the organism

Eclipta alba L. Derived Phytochemicals against Campylobacter Causing Diarrhea

Phytochemicals from Eclipta albaL.plant extract are traditionally used to cure Diarrhea. It is caused by Campylobacter. Molecular docking method applied using “Biovia Discovery Studio”. “High positive values of -CDocker energy and -CDocker interaction energy” suggested that 6, 10, 14-trimethyl-2-pentadecanone can effectively deactivate the Argininedecarboxylase enzyme (protein database code 3N29) thereby interrupting the life cycle of the organism

Modulated UV-B radiation invoking cytomorphological and biochemical aspects of a medicinal plant, Bhringraj (Eclipta alba (L.) Hassk.)

Aim: The current experimental work has been designed to study the effect of UV-B exposure on the seedling growth and meiotic consequences of Eclipta alba (L.) Hassk.Methodology: The seedlings were exposed to UV-B radiation for different time durations, i.e., 40, 80, 120 min along with control sets. UV-B irradiated seedlings along with respective controls were sown in the field and young floral buds were fixed in Carnoy’s fixative for 24 hrs and preserved in 90% ethanol for meiotic study. Results: Exposure of UV-B exposure resulted in various chromosomal aberrations like stickiness, laggards, bridges, unorientation, precocious, multivalents etc. Chromosomal stickiness was profound abnormality encountered at shorter duration (40 min) to UV-B exposure. The results revealed that UV-B exposure for shorter duration is quite beneficial to plant as it induces significant cytomorphological and biochemical variations. Interpretation: Shorter exposure to UV-B radiation induced certain beneficial traits in Eclipta alba. Since, Eclipta alba is a medicinally significant plant, hence, it is essential to improve their quantitative and qualitative traits through induced mutagenesis using UV-B radiation to impel the novel characteristics of plant

In Vitro Study Of An Aqueous Extract Of Eclipta Alba Hassk. For Hepg2 Cell Line

It is the need of the day to identify the new anticancer herbal drug, which not only in possession of good anticancer effects but also cost effective. Here we are presenting such an anticancer Ayurvedic herb which is used since the centuries for the treatment of different diseases of diverse origin. Eclipta alba Hassk., also called as Bhringraj is very important medicinal herb in many medicinal formulations. Though it is commonly used for hair growth, many evidences found its hepatoprotective activity. Here we are presenting all aspects about Bhringraj in terms of qualitative and quantitative values and we have also tried to prove the anticancer activity of it for hepatic cancer. We have used the aqueous extract of Eclipta alba Hassk. for phytochemical analysis, TLC, HPLC analysis to test active chemical components in it. Extract showed presence of many active chemical components which were responsible for its anticancer activity. In vitro study we used the aqueous extract of Eclipta alba Hassk. for the evaluation of its effects on HepG2 (Human liver cancer cell line). The SRB assay results were used to evaluate the anti-cancer activity of the extract. The effects of whole plant extract on cancer cell line were studied. Percentage of cell growth and cell viability were calculated from tabulated result values of srb assay. The experiment revealed that the average percentage of growth inhibition was 68.74%. Cell viability SRB assay also showed significant growth inhibition, at the same time statistical analysis of SRB assay also proved significant results. The research performed here is very useful for set up of different extract studies of Bhringraj for its anticancer activity.

Influence of Eclipta alba on serum alanine aminotransferase and aspartate aminotransferase activity in rabbits

Background: Paracetamol is a recognized antipyretic, analgesic drug which produces hepatic necrosis in high doses. Eclipta alba elaborates a vast array of biologically active compounds that are chemically diverse and structurally complex.Methods: Randomized open controlled experimental study Estimated levels of Serum aspartate aminotransferase (AST), Serum alanine aminotransferase (ALT) and Hepatoprotective action of in High doses of Paracetamol on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity.Results: ALT in all the groups including Control group (A) was (51.8±4.56IU/L). Paracetamol treated group (B) the ALT level increased at 48 hours and continued to be high up to 60 days (136.4±20.73IU/L) then decreased to (113.7±11.35IU/L) at 90 days. AST in all the groups including Control group (A) was (22.5±1.23IU/L). Appropriate antioxidant in appropriate doses as a matter of routine whenever hepatotoxic or potentially hepatotoxic drugs are prescribed. In Paracetamol treated group (B) the AST level increased at 48 hours and continued to be high up to 60 days (99.4±9.73IU/L) then decreased to (85.4±7.39IU/L) at 90 days.Conclusions: Appropriate antioxidant in appropriate doses as a matter of routine whenever hepatotoxic or potentially hepatotoxic drugs are prescribed.

Hepatoprotective activity of Eclipta alba against carbon tetrachlorideinduced hepatotoxicity in albino rats.

Background: Despite the tremendous scientific advancement in the field of gastroenterology over the recent years, there is not even a single effective allopathic medication available for the treatment of liver disorders. Hence, the study was conducted to elucidate the hepatoprotective activity of aqueous extract of traditional medicinal plant Eclipta alba against carbon tetrachloride (CCl4) induced toxicity in male albino rats. Methods: The hepatoprotective effect of the aqueous extracts of E. alba was evaluated by biochemical parameters such as serum alanine transferases (ALT), serum aspartate transferases (AST), alkaline phosphatase (ALP), total serum bilirubin, and serum protein, and confirmed by histopathology of liver. The hepatotoxic agent CCl4 was used to induce liver toxicity and silymarin was used as a control drug. The aqueous extracts of E. alba were administered at the doses of 250 mg/kg/day and 500 mg/kg/day orally for 4 days. One-way Analysis of Variance was used for the statistical analysis of data. A probability value of p<0.05 was considered as significant. Results: E. alba administration at doses 250 mg/kg and 500 mg/kg orally demonstrated significant hepatoprotective activity by preventing the increase of ALT, AST, ALP, and serum bilirubin and also confirmed by histopathology of the liver. The results were comparable to that of silymarin. Conclusion: The results of the study confirmed the hepatoprotective activity of aqueous extracts of E. alba at doses of 250 mg/kg and 500 mg/kg against CCl4 induced hepatotoxicity in rats. However, the dose adjustments may be necessary to optimize the similar hepatoprotective efficacy in clinical settings.

Effect of Eclipta alba on learning and memory ability and brain derived neurotrophic factor with Alzheimer's rats / 中华行为医学与脑科学杂志

Objective To study the effect of Eclipta alba on learning and memory ability and brain derived neurotrophic factor(BDNF) in brain of Alzheimer's disease (AD) model rats.Methods Healthy adult SD rats were randomly divided into control group,model group,low dosage group and high dosage group.There were 10 rats in each group.The model of Alzheimer's disease was established with subcutaneous injection of D-galactose and microinjection Aβ25-35 on bilateral hippocampus.All rats were treated with saline solution or different dosage of Eclipta alba respectively lasting 8 weeks.Then the ability of learning and memory of AD rats was evaluated by the Morris water maze test.The levels of expression of BDNF in brain were determined by immunohistochemical staining method and Western Blot method.Results The Morris water maze test:the average escape latent period prolonged besides the percentage of the swimming time in the target quadrant from the total swimming time and the times across the platform((34.14± 1.43) s,(33.71±3.82) ％,(3.40±0.70) times) decreased significantly in model group compared with control group((18.83±0.62) s,(41.98±3.96) ％,(5.40± 1.17) times,P＜0.01).The average escape latent period shortened besides the percentage of the swimming time in the target quadrant from the total swimming time and the times across the platform increased significantly in high dosage group compared with the model group (P＜0.01).Immunohistochemical staining and Western blot:the level of expression of BDNF in brain in model group was prominently less than control group (P＜ 0.01).The BDNF level in drug treated groups was prominently higher than model group(P＜0.01).The expression of BDNF increased with the drug dosage increasing.Conclusion Eclipta alba can improve the learning and memory function of AD rats by enhance the expression of BDNF.

Pharmacological Activities of Eclipta Alba(L.).

Eclipta Alba (L.) is small branched annual herbaceous plant with a long history of traditional medicines uses in many countries especially in tropical and subtropical regions. The herb has been known for its curative properties and has been utilized as antimytotoxic, analgesic, antibacterial, antihepatotoxic, antihaemorrhagic, antihyperglycemic, antioxidant, immunomodulatory properties and it is considered as a good rejuvenator too. Recent studies showed an antivenom property & corrosion pickling inhibitor action on mild steelin hydrochloric acid. A wide range of chemical compounds including coumestans, alkaloids, thiopenes,flavonoids, polyacetylenes, triterpenes and their glycosides have been isolated from this species. Extracts and metabolites from this plant have been known to possess pharmacological properties. This contribution provides an comprehensive review on ethnomedicinal uses, chemical composition, and the pharmacological profile as medicinal plant. Particular attention is given to antihepatotoxic, analgesic, antioxidant , antihyperglycemic, antiaggresive, wound healing properties and insecticidal effects presented in this review such that the potential use of this plant either in pharmaceutics or as an agricultural resource can be evaluated.

Development and validation of a rapid high performance liquid chromatography - photodiode array detection method for estimation of a bioactive compound wedelolactone in extracts of Eclipta alba

Following optimization of extraction, separation and analytical conditions, a rapid, sensitive and simple reverse-phase high performance liquid chromatography-photo diode array (HPLC-PDA) method has been developed for the identification and quantification of wedelolactone in different extracts of Eclipta alba. The separation of wedelolactone was achieved on a C18 column using the solvent system consisting of a mixture of methanol: water: acetic acid (95: 5: 0.04) as a mobile phase in isocratic elution mode followed by photo diode array detection at 352 nm. The developed method was validated as per the guidelines of the International Conference on Harmonization (ICH). Calibration curve presented good linear regression (r²>0.998) within the test range and the maximum relative standard deviation (RSD, %) values for intra-day assay were found to be 0.15, 1.30 and 1.1 for low (5 µg/mL), medium (20 µg/mL) and high (80 µg/mL) concentrations of wedelolactone. For inter-day assay the maximum RSD (%) values were found to be 2.83, 1.51 and 2.06 for low, medium and high concentrations, respectively. Limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 2 and 5 µg/mL respectively. Analytical recovery of wedelolactone was greater than 95%. Wedelolactone in different extracts of Eclipta alba was identified and quantified using the developed HPLC method. The validated HPLC method allowed precise quantitative analysis of wedelolactone in Eclipta. alba extracts.

Desenvolveu-se método rápido, sensível e simples de Cromatografia Líquida de Alta Eficiência em fase reversa, utilizando-se arranjo de fotodiodo (HPLC-PDA), visando à separação, extração e às condições analíticas para a identificação e quantificação de wedelolactona em diferentes extratos de Eclipta alba. A separação de wedelolactona foi efetuada por meio de uma coluna C18, utilizando mistura de metanol:água:ácido acético (95:5:0.04) como fase móvel, em sistema de eluição isocrática, seguida de detecção por arranjo de fotodiodo a 352 nm. O método desenvolvido foi validado de acordo com as diretrizes da Conferência Internacional de Harmonização (ICH). As curvas de calibração apresentaram boa regressão linear (r²>0,998), dentro dos intervalos de teste, e os valores máximos de desvio padrão relativo (RSD,%) dos ensaios intra-dia foram 0,15, 1,30 e 1,1 para concentrações de wedelolactona baixa (5 µg/mL), média (20 µg/mL) e elevada (80 µg/mL) Para o ensaio inter-dia,os máximos de RSD (%) foram 2,83, 1,51 e 2,06 para as concentrações baixa, média e alta, respectivamente. O Limite de Detecção (LD) e o Limite de Quantificação (LOQ) foram de 2 e 5 µg/mL, respectivamente. A recuperação analítica de wedelolactona foi maior do que 95%. A wedelolactona em diferentes extratos de Eclipta alba foi identificada e quantificada pelo método de HPLC desenvolvido. O método de HPLC validado permitiu a análise quantitativa precisa de wedelolactona em extratos de Eclipta alba.

Screening of eclipta alba extracts for anticancer activity.

Cancer is the second largest cause of death which killed 7.6 million people worldwide in 2005 (Danaei et al., 2005). The number is believed to become 9 million in 2015 and 11.5 million in 2030 (World Health Organization, 2007). There is an increasing interest to research and develop on new anticancer drugs, from both synthetic and natural sources (Mukherjee et al., 2001). Half of the drugs which have been approved recently are from natural sources. Plants have been extensively used as natural sources to develop anticancer drugs because of their active constituents. Medicinal plants in Asian countries play an important role in cancer treatment and indeed, their chemical constituents and derivatives have been utilized for combating cancers over the last half-century. Presently in the study Eclipta alba ethanolic plant extract has been used for screening the anti-cancer activity in-vitro and the results obtained, gives the evidence that the ethanolic extract inhibit the growth of colon cancer cells at concentrations that showed no apparent toxicity effect against normal cells.

Human immunodeficiency virus (HIV-1) reverse transcriptase inhibitory activity of eclipta alba (L) leaves extracts.

Highly active anti-retroviral therapy (HAART) is the current HIV/AIDS treatment modality. Despite the fact that HAART is very effective in suppressing HIV-1 replication and reducing the mortality of HIV/AIDS patients, it has become increasingly clear that HAART does not offer an ultimate cure to HIV/AIDS. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. This need has further manifested by recent clinical trial failures in anti-HIV-1 vaccines and microbicides. . In the current study, we characterized a panel of extracts of traditional medicinal plants for their activities against HIV-1 replication. The aim of the present study was to evaluate the invitro anti- HIV activity of Eclipta alba plant extracts. Extracts were prepared from dried fruits in n-hexane, ethyl acetate and n butanol. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts by MTT assay using PBMCs isolated from whole blood. HIV-1 RT inhibition activity of the all solvent extracts of Eclipta alba was determined by a RetrsoSys HIV-1 RT activity kit (Innovagen, Sweden). The aerial parts of Eclipta alba extracts are shows anti-HIV-1 activity and this plant has great potential for developing useful drugs.

Atividade alelopática de extratos aquosos de plantas medicinais na germinação de Lactuca sativa L. e Bidens pilosa L. / Allelopathic activity of aqueous extracts of medicinal plants on the germination of Lactuca sativa L. and Bidens pilosa L.

O potencial alelopático de quatro diferentes extratos de plantas medicinais (Eclipta alba, Gomphrena globosa, Tabernaemontana catharinensis e Tithonia diversifolia) foi avaliado na germinação de Bidens pilosa e Lactuca sativa. Dentre os resultados obtidos no teste de germinação, notou-se um maior poder inibitório do extrato de G. globosa sobre sementes e plântulas de Bidens pilosa e Lactuca sativa independente da concentração utilizada e aumento da atividade inibitória de extratos T. catharinensis e de T. diversifolia com aumento da concentração destes. Quanto ao Índice de Velocidade de Germinação, os dados demonstraram redução do índice devido a elevada mortalidade das plântulas submetidas aos extratos de G. globosa e de T. catharinensis e a germinação tardia das sementes submetidas ao extrato de T. diversifolia. Quanto ao vigor das sementes, as menores porcentagens de sobrevivência ocorreram extratos à 2,5 por cento para B. pilosa e 10 por cento para L. sativa.

The allelopathic potential of four different extracts of medicinal plants (Eclipta alba, Gomphrena globosa, Tabernaemontana catharinensis and Tithonia diversifolia) was evaluated on the germination of Bidens pilosa and Lactuca sativa. The results obtained in the germination test indicated a greater inhibitory activity of G. globosa extract on Bidens pilosa and Lactuca sativa seeds and seedlings, regardlessof the used concentration, and an increase in the inhibitory activity of T. catharinensis and T. diversifolia extracts with an increase in their concentrations. As to Germination Speed Index, the data demonstrated a reduction due to the high mortality of seedlings subjected to G. globosa and T. catharinensis extracts and a late germination of seeds subjected to T. diversifolia extracts. As to the vigor of seeds, the smallest survival percentages occur at 2.5 percent for B. pilosa and at 10 percent for L.sativa.

Evaluation of mollucicidal activity of some Indian medicinal plants against lymnaea acuminata.

Molluscicidal property of E. alba, B. aegyptiaca, C.quandragularis against the snails Lymnaea acuminata was studied. It was observed that the toxicity of different preparation for molluscs was both time and dose dependent. On the basis of these results it may conclude that, the Ethanolic extract of E.alba may be used for the pest management, however, further animal study is needed to confirm its physiological toxicity.