EFFECT OF AN INCREASED SUPPLEMENTATION OF EGG WHITE PROTEIN ON THE POST-EXERCISE LEUKOCYTE SUBSETS AND THE PLASMA IL-6 LEVELS IN MALE COLLEGIATE RUNNERS / 体力科学

This study was undertaken to compare the effect of the pre-exercise intake of a dry egg white protein supplement (86 kcal) on the post-exercise alterations in leukocytes and the plasma interleukin-6 (IL-6) concentrations in male collegiate runners. The 43 runners who participated in this study were divided into 2 groups, namely those with (E groups) and without (C groups) a 12,000 m pace run, and then they were further divided into 4 subgroups in which the subjects were given the 86 kcal nutrient supplemented including either 0, 5, 10, or 20 g protein from egg white. Peripheral blood samples were collected before supplementation (Pre), just after running (Post 0h), and at 1 h after running (Post 1h). The concentrations of the leukocytes, neutrophils, and monocytes in both groups E and C remained unchanged at Post 0 h. Furthermore, these concentrations increased significantly at Post 1 h. The lymphocytes were not significantly different at Pre. The plasma IL-6 concentrations were significantly higher at Post 0 h than at Pre or Post 1 h. In addition, no effect of the protein supplementation on the leukocytes, leukocytes subsets, or the IL-6 concentrations was observed. These observations suggested that such isocaloric supplementation as described above may therefore be able to help control the changes in the concentrations of leukocyte and leukocyte subsets immediately after running a 12,000 m time trial.

STUDY ON FREE RADICAL SCAVENGING ACTIVITY OF PROTEINASE HYDROLYSATES FROM EGG WHITE / 营养学报

trypsin. The EWPHs with papain for 3h displayed the strongest radical scavenging activity. The values of hydroxyl radical and superoxide free radical scavenging activity were 65.63% and 38.40% respectively. Conclusion EWPHs exhibited free radical scavenging activity and the activity of scavenging hydroxyl radical were stronger than superoxide radical.

IgE and IgG Immunoblot Patterns to Three Major Egg Allergens : According to the Routes of Sensitization in Atopic Children(<3 Years Old) / 소아알레르기및호흡기학회지

PURPOSE: Atopic dermatitis is a chronic, inflammatory relapsing skin disease fre- quently seen in children with a history of food allergy. Recently, acute allergic reaction to egg in patients with atopic dermatitis who have never been exposed to egg white protein has been reported. The pattern of antigen-antibody reaction by means of IgE and IgG western blotting was analysed to evaluate the possibility of non- dietary sensitization. METHODS: Total 16 children(<3 years old) with egg allergy were enrolled. Eight patients of them had never ingested egg before(Non-dietary sensitization, NDS) and the other 8 patients had a history of previous egg ingestion without significant clinical symptoms(Dietary sensitization, DS). Egg proteins were analysed by SOS-PAGE and antigen-antibody reaction by means of IgE and IgG was deteded by Western-Blotting. RESULTS: The pattern of IgE antibodies to egg white protein were similar in the two groups. IgG Western blotting to egg white in the two groups, the ovotran sferrine and ovalbumin were significantly bound by 100% of sera, respectively. In DS patients, specific IgG antibodies to ovomucoid was significantly bound by 87% (7/8) of sera whereas only 38%(3/8) of sera had detectable specific IgG antibodies against ovomucoid in NDS patients. CONCLUSION: Theses results suggest that specific IgE to egg white related to exposure of egg white protein regar specific IgG to ovomucoid is related to dietary ingestion of egg. Specific IgG to ovalbumin and ovotrans-ferrine show good clinical correlation, further study might be essential to clarify to this hypothesis.