RESUMO
@#A novel electrophoretic separation system has been successfully applied for the preparation of human sperm prior to the execution of assisted reproductive techniques (ARTs). This new system is designed to overcome the generation of reactive oxygen species (ROS) through centrifugation in conventional sperm preparation. Since the previous study showed favorable outcomes in humans, this study intends to implement this new system for animal sperm preparation particularly in bull. Fresh semen from adult bulls were used. Optimization of the electrophoretic system for optimum bull sperm separation involved different strength of voltage and separation time. The voltages applied were 10V, 20V, 30V, 40V, 50V, and 60V. For each voltage applied, the system was operated for a duration of 12 min. An average of 10 µl fractionalized semen was taken out at the collection site at every 2-min interval. Every fractionated sperm was then evaluated for percentage of viability, motility, and DNA damage assessment. Result showed that electrophoresis at 20V and 6 min yielded more than 80% viable and more than 70% motile sperm population with the lowest DNA damage. In conclusion, the system was able to fractionate high quality bull sperm at 20V and 6 min.
RESUMO
Recent developments on separation and analysis of bacterial cells by capillary electrophoresis are reviewed. Based on bacterial surface characterization, capillary electrophoresis technique can be utilized for rapidly identify and quantify intact bacteria and microbes at a certain phase. The microbe-molecule interactions can also be described. The separation of microbes that can not be cultured in lab appears to be possible by this method. It makes profound effects on separation science and microbiology to establish capillary electrophoresis procedure for the separation and analysis of bacterial cells.