RESUMO
Objective To analyze the content and variation rules of 10 constituents in radix, rhizome, and leaf of Rheum officinale at one-, two-, and three-year-old stage, respectively, and provide theoretical guidance for efficient production and quality control of the crud drug. Methods The content of each constituent in R. officinale was determined by high performance liquid chromatography (HPLC), and one factor analysis of variance and multiple comparison were performed by SPSS 24.0. Results HPLC system was established for the determination of 10 components in R. officinale. The linear range was good (r2 > 0.997), RSD of precision, stability, and repeatability were less than 2%, and the recoveries were 96.10%—107.10%, respectively. The content analyses showed that, in the same part, the content of gallic acid decreased significantly year by year or at the 2nd growth years (P 3 > 2 (P 1 > 2 (P radix > leaf (P < 0.05). Conclusion The HPLC based determination of 10 constituents in R. officinale showed that the accumulation profiles of the samples at different years or from different parts varied. For the same parts, the contents of most constituents increased year by year. During the same growth year, the contents of most constituents in radix or rhizome were higher than those in leaf. The radix and rhizome of the three years old samples had the highest contents of main constituents.
RESUMO
Objective: To establish an HPLC method for determing nine components from the leaves of Rheum officinale, such as gallic acid, senna glycosides B, rhubarb phenol-1-O-glucoside, emodin-8-O-glucoside, aloe-emodin, rhein, emodin, rhubarb, and emodin methyl ether, as well as to explore the scientific evidence for reasonable exploitation and utilization of medicinal rhubarb leaf. Methods: Takeing C18 chromatographic column (250 mm × 4.6 mm, 5 microns), methanol-0.2% acetic acid water as mobile phase, gradient elution and flow rate of 1 mL/min, column temperature 30℃, and detection wavelength of 260 nm. Results: Gallic acid, senna glycosides B, rhubarb phenol-1-O-glucoside, emodin-8-O-glucoside, aloe-emodin, rhein, emodin, rhubarb, and emodin methyl ether had the good linear relationship, respectively in the range of 20.2-606.0, 79.3-2 379.0, 10.1-301.8, 14.8-443.7, 0.7-2.2, 0.13-3.9, 12.4-372.0, 38.8-1 164.0, 6.2-185.4 ng (r > 0.999 6); The nine kinds of the ingredients of the average recovery were 95.76% - 95.76%, RSD was 1.46% - 2.43%. Conclusion: This method is simple and accurate, and with good effect and reliable results for the separation and determination of the components from the leaves of R. officinale, which can provide the reference for its rational development and utilization.
RESUMO
Objective: To research the influence of prescription compatibility on the extraction rate of main constituents in Yinchenhao Decoction (YCHD). Methods: The extraction volume of two iridoids (geniposide and deacetylasperulosidic acid methyl ester), two crocetin derivatives (crocin I and crocin II), three bound anthraquinones (aloe-emodin-8-O-glucoside, chrysophanol-1-glucoside, and emodin-8-glucoside), five free anthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion), two tannins (gallic acid and catechin), and chlorogenic acid in Artemisiae Scopariae Herba (ASH), Gardeniae Fructus (GF), Rhei Radix et Rhizoma (RRR), the compatibility of ASH and GF (compatibility A), the compatibility of ASH and RRR (compatibility B), the compatibility of GF and RRR (compatibility C), and the compatibility of ASH, GF, and RRR (compatibility D) were determined by HPLC. Taking the extraction volume in single medicine as 100%, the extraction rates of 15 constituents mentioned above in compatibilities A-D were calculated. Results: The extraction rates of geniposide, crocin I, and crocin II in compatibilities A, C, and D, deacetylasperulosidic acid methyl ester in compatibility C, gallic acid in compatibilities C and D, and chlorogenic acid in compatibilities A-D decreased. The extraction rates of deacetylasperulosidic acid methyl ester in compatibilities A and D, three bound anthraquinones, aloe-emodin, and chrysophanol in compatibility B, chrysophanol and physcion in compatibilities C and D increased. Conclusion: Prescription compatibility has some influence on the extraction rate of main constituents in YCHD.