RESUMO
Objective To analyze the effect of antimicrobial enema liquid to explore different bacterial infection patients with chronic pelvic inflammatory disease by clearing away heat and promoting diuresis, detumescence,promoting blood circulation and relieving pain, and to investigate the influence of antimicrobial enema liquid applicated in rectal on endovaginal microecologics.Methods Seventy-one cases of chronic pelvic inflammatorydisease patients were selected as the research objects in Obstetrics and Gynecology Hospital Affiliated to Fudan University from March 2011 to January 2012.The bacterial culture of cervical secretions in patients with chronic pelvic inflammatory disease(excluding the vulva and vaginitis) was swab by cotton swab, then bacterial culture and bacterial vaginal infection Wulian analysis were conducted.The minimal inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of the common pathogenic bacteria were determined by using the flat dish two times dilution method according to the different kinds of pathogenic bacteria.Wulian analysis bacterial vaginal infection was reviewed after patients received the same kind of enema rectal administration.Results Main pathogenic bacteria in 71 cases of patients with chronic pelvic inflammatory disease were enterococcus faecalis of 12.5％ (4/71), beta hemolytic streptococcus of 31.25％ (10/17), escherichia coli of 34.38％ (11/71), Staphylococcus aureus of 28.13％ (9/71), mlicrococcus scarlatinae of 6.25％ (2/71), bacillus typhosus of 6.25％ (2/71), Shigella of 3.13％ (1/71), staphylococcus epidermidis of 3.13％ (1/71), klebsiella pneumoniae of 3.13％ (1/71) and pseudomonas aeruginosa of 3.13％ (1/71) .Chronic pelvic inflammatory disease enema on enterococcus faecalis, beta hemolytic streptococcus, escherichia coli, staphylococcus aureus and antibacterial activity was obvious, the minimum inhibitory concentration (MIC) were the original drug concentration of 1/32,1/32,1/16, 1/16, the minimum bactericidal concentration(MBC) respectively as raw material concentrations of 1/16, 1/8, 1/8, 1/8;has certain antibacterial activity against streptococcus pyogenes, typhoid salmonella, shigella flexneri, the MIC were the original drug concentrations of 1/8,1/8,1/4,the MBC were the original drug concentrations of 1/8,1/4,1/4.In vitro antibacterial activity on pseudomonas aeruginosa, coagulase negative staphylococci was not obvious.There were statistically significant differences in PH activity abnormality rate (67.61％ vs 36.62％), positive rate of catalase (66.20％ vs 29.58％), leukocyte esterase (56.33％ vs 29.58％), neuraminidase (53.52％ vs 25.35％), proline aminopeptidase (57.75％ vs 32.39％) and glucosaminidase(52.11％ vs 22.55％) after enema liquid applicated in rectal, and the difference was significant(P＜0.5).Conclusion The use of clearing away heat and promoting diuresis, detumescence, Huoxue Zhitong enema on chronic pelvic inflammatory disease pathogenic bacteria have certain inhibition, especially enterococcus faecalis infection.The drug has efficient on chronic pelvic inflammatory disease and endovaginal microecologics.