RESUMO
AIM To develop an HPLC method for the simultaneous content determination of narirutin,hesperidin,nobiletin,3,5,6,7,8,3',4'-heptemthoxyflavone,tangeratin,dehydrotumulosic acid,dehydropachymic acid and pachymic acid in Erchen Mixture (Citri reticulatae Pericarpium,Poria,Pinelliae Rhizoma,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 0.9 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.94％-99.69％ with the RSDs of 0.88％-1.76％.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Erchen Mixture.
RESUMO
Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-acetic acid-water(22∶4∶74). The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg(r=0.999 8). The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.