RESUMO
In order to study the alkaloids from branches and leaves of Ervatamia hainanensis, silica gel, ODS, Sephadex LH-20 and HPLC chromatography were used to obtain six alkaloids from the branches and leaves of E. hainanensis with use of. Based on the physicochemical properties and spectral data, their structures were identified as 10-hydroxydemethylhirsuteine(1), 3R-hydroxycoronaridine(2), 3-(2-oxopropyl)coronaridine(3), pandine(4), 16-epi-vobasine(5), and 16-epi-vobasinic acid(6). Among them, compound 1 was a new monoterpenoid indole alkaloid, and compounds 5 and 6 were obtained from this plant for the first time.
Assuntos
Alcaloides , Cromatografia Líquida de Alta Pressão , Estrutura Molecular , Folhas de Planta , TabernaemontanaRESUMO
Objective: To study the alkaloids from Ervatamia hainanensis. Methods: The alkaloids were isolated and purified by silica gel, MCI, Sephadex LH-20 column chromatography, and semi-preparative HPLC, and their structures were elucidated by physical and spectroscopic analysis. Results: Twelve alkaloids were obtained and identified as coronaridine (1), 19-epi-heyneanine (2), 9,10-dimethoxycoronaridine (3), vobasine (4), vobasine N(4)-oxide (5), 3-oxo-19-epi-heyneanine (6), strictamine (7), deacetylakuammiline (8), pandine (9), rhazicine (10), rhazicine N(4)-oxide (11), and rhazimine (12). Conclusion: Compounds 8 and 10-12 are isolated from the genus Ervatamia Stapf for the first time, while compounds 3 and 5-7 are firstly obtained from E. hainanensis.
RESUMO
Objective: To study the chemical constituents in the twigs and leaves of Ervatamia hainanensis. Methods: The chemical constituents were isolated by silica gel, ODS, Sephadex LH-20 column chromatographies, and preparative HPLC. The structures were elucidated on the basis of physicochemical properties and spectral analysis. Results: Four lignans were isolated and identified as threo-7-O-ethylguaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (1), threo-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (2), erythro-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (3), and dehydrodiconiferyl alcohol (4). Conclusion: Compound 1 is a new 8-O-4'-neolignan and named as hainanervaligan A (1), and compounds 2-4 are obtained from the plants of Ervatamia (A. DC.) Stapf for the first time.
RESUMO
Objective: To investigate the chemical constituents in the twigs and leaves of Ervatamia hainanensis. Methods: The chemical constituents were isolated by silica gel, Sephadex LH-20, and ODS columns, as well as RP-HPLC chromatography. Their structures were identified on the basis of physicochemical properties and spectral analyses. Results: Thirteen compounds were isolated and idenified as coronaridine (1), 19-heyneanine (2), 19-epi-heyneanine (3), coronaridine hydroxyindolenine (4), vobasine (5), ursolic acid (6), asiatic acid (7), 8α-hydroxypinoresinol (8), (+)-syringarosinol (9), ervatamisin [(-)-(7R, 7'R, 7″R, 8S, 8'S, 8″S)-4', 4″-dihydroxy-3, 3', 3″, 5-tetramethoxy-7, 9', 7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineolignan-7″, 9″-diol, 10], toddalolactone (11), threo-2, 3-bis-(4-hydroxy-3-methoxypheyl)-3-methoxy-propanol (12), and β-sitosterol (13). Conclusion: Compounds 6-8 and 10-12 are isolated from the plants of Ervatamia (A. DC.) Stapf for the first time, and compound 9 is firstly reported from E. hainanensis.
RESUMO
Objective To study indole alkaloids of Ervatamia hainanensis. Methods The compounds were separated and purified by column chromatography with silica gel and Sephadex LH-20. The structures were identified by IR, MS, and NMR. Results Seven alkaloids were identified as: hainanervatasine (Ⅰ), hainanervatacine (Ⅱ), vobasine (Ⅲ), coronaridine (Ⅳ), 3-hydroxyl coronaridine (Ⅴ), 3-(2-oxopropyl) coronaridine (Ⅵ), and tabernamine (Ⅶ), respectively. Conclusion Compounds Ⅰ and Ⅱ are new compounds. Compounds Ⅳ and Ⅵ are isolated from this plant for the first time.