RESUMO
In order to investigate the immune enhancement effects of Ophiopogon japonicus polysaccharide Ophiopogon japonicus (OJPS) on Newcastle disease (ND) live vaccine, chickens vaccinated against ND live vaccine was orally administered with the OJPS at high, medium and low concentrations respectively. In negative control group, chickens were given orally equal volume of physiological saline. On day 14, 21 and 28, the serum antibody titer, erythrocyte-C3b receptor rosette rate (E-C3bRR), erythrocyte-C3b immune complex rosette rate (E-ICRR) and peripheral lymphocyte proliferation were measured. The results showed that at most time points, the antibody titer, peripheral lymphocyte proliferation, E-C3bRR and elimination rate of immune complex of three OJPS administrating groups were significantly higher (P<0.05) than those in negative control group. It indicated that OJPS could significantly improve the immune efficacy of Newcastle disease live vaccine, Ophiopogon japonicus polysaccharide possessed synergistical immunoenhancement.(AU)
Assuntos
Animais , Galinhas/virologia , Doença de Newcastle/imunologia , Ophiopogon/química , Vacinas Virais/análise , Adjuvantes Imunológicos , Anticorpos/sangue , Eritrócitos/imunologia , Linfócitos/imunologiaRESUMO
Objective To study the expression of interleukin-2 (IL-2), soluble interleukin-2 receptor (sIL-2R),determine the alteration of erythrocytic immunity and T cell subgroup in the blood of outer circulation in patients with hypertensive cerebral hemorrhage so and to probe into the relationship between them, and to explore the clinical significance. Methods Enzyme linked immnunosorbent assay (ELISA) was used to determine the content of IL-2 and sIL-2R. The immunoadsorption was employed to examine the erythrocytic immune activity and its regulating function.Streptavidin-peroxidase(S-P) was used to determine the cell number of CD3 (cluster of differentiation3), CD4 and CD8. Results The content of IL-2 in the group with hypertensive cerebral hemorrhage was significantly lower than that in the control group (P＜0.01), and the content of sIL-2R increased. Red blood cell C3b receptor (RBC. C3b R)and RBC immune adherence enhancing factor (RFEB) dropped greatly (P＜0.01), while RBC immune complex rosette (RBC. ICR) and RBC immune adherence inhibiting factor (RFIR) increased greatly. The cell number of CD3 and CD4decreased (P＜0.01) and there was no obvious change in CD8 (P＜0.05). Conclusion The decrease of immune function was observed in patients with hypertensive cerebral hemorrhage. The determination of the content of IL-2, sIL-2R, erythrocytic immunity and the activity of T subgroup has an important clinical significance in the occurrence,development, treatment, and prognosis of hypertensive cerebral hemorrhage.
RESUMO
Objective: Through treatment based on differentiation of symptoms and signs and classification of defi nited patients of maternofetal blood group incompatibility, detecting anti-A or Anti-B antibody and erythrocyte immune complex chaplet rate(RICR) and erythrocyte receptor chaplet rate(RC3bR) , etc, to observe the therapeutic effect and mechanism of prescriptions mainly with Yinchenhao Decoction. Methods: Patients definited maternofetal blood group incompatibility were divided into control group and observation group according to random principle in 1:3 ratio. Then, observation group was divided into moist heat group(SRG), moist heat and defi ciency of spleen qi group (SR+PQXG) and moist heat and defi ciency of kidney qi group(SR+SQXG) according to syndrome differentiation. Corresponding prescriptions were used, every 30 doses 1 course. Before and after treatment, the anti-A or anti-B antibody and RICR, RC3bR were detected in peripheral blood. Results: In Yinchenhao Decoction group with 21 cases, the anti-A or anti anti-B antibody decreased, including 5 case (1:64) and 4 case (1:32). In group of syndrome differentiation with 63 cases, the anti-A or anti anti-B antibody decreased, including 33 case(1:64) and 20 case (1:32). Before and after treatment, RICR of control group and observation group were(19.43?5.29), (19.57?6.50)and (20.59? 7.96), (23.77?5.12), respectively. RC3bR were(11.62?4.16), (12.42?6.60)and(11.93?4.10), (13.96?6.40)respectively. There was signifi cant difference of before and after treatment in obserbation group(P0.05). Conclusion: In cutting down anti-A and anti-B antibody, and increasing RICR and RC3bR fi eld, the observation groups were better than control group.
RESUMO
Objective To study the content of interleukin- 6 (IL-6) and the altering pattern of erythrocyte immunity and T cell subgroup in the blood of outer circulation in the patients with cerebral glioma, and to expl ore their relationship and the clinical significance. Methods Enzyme linked immunosorbent assay (ELISA) was used to determine the content o f IL-6. The immunoabsorption was employed to examine the erythrocytic immune ac tivity and its regulatory function. Streptavidin-Peroxidase (S-P) was used to determine the cell number of CD3 (cluster of differentiation 3), CD4, CD8. Results The content of IL-6 in the group with cerebral glioma i s significantly lower than that in the control group (P0.05). Conclusion The weakening of immune function is obse rved in the patients with cerebral glioma. Testing of the content of IL-6, eryt hrocyte immunity and the activity of T subgroup has important clinical significa nce in the occurrence, development, treatment, outcome and prognosis of the cere bral glioma.
RESUMO
Objective:Toexplore the changes and mechanismofErythrocyte ImmunityFunction(EIF)in rats with Traumatic Brain Injury(TBI).Methods:HealthyWistar rat(sn=70)were divided intocontrol group(n=10)and TBI group(n=60),model ofTBI were prepared and divided into mild group(n=30)and moderate to severe group(n=30).Red Blood Cell-C3b Receptor Rate(RBC-C3bRR)and Red Blood Cell-Immune Complex Rate(RBC-ICR)of rats with TBI were measured by methed of yeast fungus and the content of serum ?-endophin(?-EP)was tested by radioimmunoassay.Results:Compared with the control group,RBC-C3bRR of rats with TBI decreased significantly while RBC-ICR and?-EP increased significantly(P
RESUMO
Objective To examine the change of erythrocyte immune adherence parameters during the haemodialysis and the influence of different biocompatible dialysis membranes on it.Methods We have observed the dynamic trend of erythrocyte immune adherence parameters at different hemodialysis phase by way of different biocompatible membrane.Results RBC C\-3b declined and RBC IC increased obviously in uremia patients.During the haemodialysis,RBC C\-3b declined even more in the early phase and then increased gradually;RBC IC increased gradually in early phase,and then kept steady.Cu membrane influenced the erythrocyte immune adherence function greatly,Ps membrane moderately,and PMMA membrane slightly.Conclusion The dynamic trends of erythrocyte immune adherence parameters change with the different biocompatible membrances used during hemodialysis.
RESUMO
Objective To investigate the change of erythrocyte immune function and T lymphocyte subsets in patients with uremia and the effect of hemodialysis on them. Methods Flow cytometry and immune adherence rosette method were used to measure the activity of RBC-CR1and the changes of T lymphocyte subsets in 23 uremic patients without hemodialysis, 22 uremic patients before and after sigle hemodialysis and 21 healthy subjects as the control group. Results The activity of RBC-CR1, the number of T lymphocyte subsets significantly changed in patients with uremia, and hemodialysis could partially improve this condition. There was obvious correlation between erythrocyte immune function and T lymphocyte subsets. Serum creatinine was positively relative to the value of RBC-C 3b RR(P
RESUMO
Objective In order to investigate the relationship between erythrocyte immune function and seleni- um(Se) level. Method Red blood cell immune adherence(RCIA) function,serum RCIA regulatory factor, blood Se content and activities of glutathione peroxidase(GPX) of residents in Keshan disease(KD) endemic and non-endemic areas were comparatively studied. Forty-eight residents in KD endemic area aged 13～ 16 years were divided into 2 groups. The residents in the experimental group were orally given 200μg Se daily as Se yeast for 12 weeks, and their erythrocyte Se content and activity of glutathione peroxidase (GPX),RCIA function,serum RCIA regulatory function and circulating immune complexes(CIC) content were determined. ResultsThe results showed that the rosette for- mation rates of erythrocyte and blood Se levels of the residents in KD area were significantly lower and the rosette formation inhibitory rate of serum RCIA of the residents in KD area was significantly higher than those in the non-endemic area. Erythrocyte Se contents, GPX activities and rosette formation rates of erythrocyte were sig- nificantly increased and the rosette formation inhibitory rates of serum RICA were significantly decreased after sup- plementing Se,but the difference in the contents of serum CIC was not significant. ConclusionThe increase of ery- throcyte immune function by Se-supplement might be one of the effective mechanisms in the prevention of KD by Se- supplement.
RESUMO
Objective To study the effect of Solanum nigrum alkaloid on erythrocyte immunity function. MethodsStudying the adherence between erythrocyte immunity and tumor-cell of S180 and H22 tumor-bearing mice; By determining with fluorescence polarization (P) and accounting the micro viscosity (?) with DPH as a probe the erythrocyte membrane fluidity of the S180 and H22 tumor-bearing mice could be investigated. ResultsS. nigrum alkaloid could increase the ratio of adherence anadem between erythrocyte and tumor-cell and promote the erythrocyte membrane fluidity of the two tumor-bearing mice. ConclusionThe inhibition of S. nigrum alkaloid on tumor can be accomplished through improving the erythrocyte membrane fluidity of two tumor-bearing mice and renewing erythrocyte immunity of tumor-bearing mice.
RESUMO
Fourty four specimens of maxillary mucosa from 36 patients with chronic maxillary sinusitis were examined in order to observe whether and where immune complexes are deposited in and to assess the quantity of various immunoglobulins (IgG, IgM, IgA) bearing cells with immunofluorescence technique. Erythrocyte immune adherent function and circulating immune complex (CIC) level were also measured in patients with chronic maxillary sinusitis. The results showed that CIC level was increased and erythrocyte immunological function was disturbed, which led to a lot of immune complexes depositing in maxillary mucosa. The results reveal the relationship between chronic sinusitis and type Ⅲ allergic reaction.
RESUMO
Objective: To investigate the influence and mechanism of zinc toxicity on the stucture and function of immune organs in ducks. Methods: By establishing the pathological model of zinc toxicity in ducks, some immunological methods, light and electron microscopes were used to check the changes in the stucture and function of immune system. Results: (1) The body weight of ZT group was significantly lower than that of ZC group after 3 weeks (P