RESUMO
The present work used paramorphic forms of Neurospora crassa 74A to remove erythrosine. The fungus culture was grown in medium containing the dye, as only carbon source for 2 and 90 h of interaction. A washing process using distilled water isolated the cellular mass mycelia was dried for 12 h at 105ºC and transformed in fine powder and analyzed in FTIR. The supernatant was analyzed through spectrophotometer UV-Vis and FTIR. Significant differences in the spectrum of UV-VIS and FTIR were observed between the control and the supernatant and between wall control and the walls colored by red, in FTIR for 2 and 90 h. Some significant bands were modified, suggesting the possibility of enzymatic biodegradation in proportion to the time of contact between the dye and fungal biomass.
O presente trabalho utilizou formas paramorfogênicas de Neurospora crassa 74A linhagens, na remoção do corante "Erythrosin B". O fungo, induzido química e fisicamente em forma de "pellets", foi usado no estudo da biodegradação deste corante. A cultura fúngica foi crescida em meio contendo o corante, como única fonte de carbono por 2 e 90h de interação. As paredes celulares foram isoladas por um processo de lavagem em água destilada e o micélio fresco foi secado por 12 h a 105ºC, e transformado num pó fino, e analisado em FTIR. O sobrenadante foi analisado através de espectrofotômetro UV-VIS e FTIR. Diferenças significativas no espectro UV-VIS e no FTIR foram observadas entre o controle e o sobrenadante e entre o controle e as paredes coloridas de vermelho e em FTIR no tempo de 2 e 90 h. Algumas bandas foram modificadas sugerindo a possibilidade de uma biodegradação enzimática em função do tempo de contato entre o corante e a biomassa fúngica.
RESUMO
BACKGROUND: Reusable Proseal(TM) laryngeal mask airways (PLMAs) can act as a vector for the transmission of prion diseases such as variant Creutzfeldt-Jacob disease. This study tested the hypothesis that supplementary ultrasonic cleaning facilitates the removal of protein deposits on PLMAs after anesthesia. METHODS: After clinical use, 40 PLMAs were randomly allocated into two groups. In the first group, the PLMAs were washed by hand and were then subsequently placed in an autoclave at 134degrees C for 40 min (Group 1, n = 20). In the second group, the PLMAs were washed by hand and ultrasonic cleaning using an enzymatic solution for 5 min, and were then subsequently placed in an autoclave (Group 2, n = 20). In both groups, protein deposits were detected on PLMAs by erythrosin staining. A staining score designated as none (0%), mild (0-20%), moderate (20-80%) and severe (80-100%), was assigned to each site (outer surface, inner surface and edges of the cuff, airway and drain tube, finger strap) according to the percentage of the stained surface area. RESULTS: Despite the cleaning of the masks, residual protein was found on the outer surface, inner surface and edge of the cuff, airway and drain tube, and finger strap of the PLMAs in both groups. Similar scores were observed for each part of the cleaned PLMAs in both groups, except for the outer surface of the PLMAs in Group 2 (P < 0.05). CONCLUSIONS: We conclude that the use of an ultrasonic cleaner with an enzymatic solution may be effective to cleanse the outer surface of the PLMAs, but there were no differences in the total scores for both groups.