Actividad antibacteriana de un gel experimental de Eucalyptus globulus Labill frente a Porphyromonas gingivalis / Antibacterial activity of an experimental Eucalyptus globulus Labill gel against Porphyromonas gingivalis

Introducción: Las plantas medicinales han demostrado poseer propiedades antibacterianas para el control de la periodontitis. Objetivo: Determinar la actividad antibacteriana frente a Porphyromonas gingivalis ATCC 33277 de un gel experimental compuesto por aceite esencial de Eucalyptus globulus Labill. Métodos: Se realizó un estudio experimental in vitro. Se empleó el programa EPi InfoTM para el cálculo de las repeticiones. El aceite esencial se obtuvo por el método de arrastre de vapor; se identificó su composición química por cromatografía de gases acoplada a espectrometría de masas. Se evaluó la concentración mínima inhibitoria (CMI) y concentración mínima bactericida (CMB). Se realizó un ensayo de difusión en Agar para medir los halos de inhibición del gel experimental al 4,46 por ciento frente a P. gingivalis, la comparación con clorhexidina al 0,12 por ciento se evaluó con la prueba U de Mann-Whitney. Se adoptó un nivel de significancia del 5 por ciento . Resultados: Se identificaron 11 constituyentes en el aceite esencial, los principales componentes químicos fueron 3-heptadecene, (Z)- (36,13 por ciento ), 1-tridecene (14,7 por ciento ) y 1,8-cineole (9,72 por ciento ). La CMI del aceite esencial fue 36,195 mg/mL y la CMB fue 39,114 mg/mL. Los halos de inhibición del gel experimental de P. gingivalis fueron 25,533 mm ± 0,960. mm. Se observaron diferencias estadísticamente significativas frente a clorhexidina al 0,12 por ciento (23,282 ± 0,345) (p < 0,05). Conclusiones: El gel experimental al 4,46 por ciento compuesto por aceite esencial de Eucalyptus globulus Labill presentó una actividad antibacteriana importante frente a Porphyromonas gingivalis ATCC 33277(AU)

Introduction: Medicinal plants have proved to have antibacterial properties for the control of periodontitis. Objective: Determine the antibacterial activity against Porphyromonas gingivalis ATCC 33277 of an experimental gel composed of essential Eucalyptus globulus Labill oil. Methods: An in vitro experimental study was conducted. The software EPi InfoTM was used to estimate the repetitions. The essential oil was obtained by steam entrainment, and its chemical composition was determined by gas chromatography / mass spectrometry. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also evaluated. An agar diffusion test was performed to measure the inhibition haloes of the 4.46 percent experimental gel against P. gingivalis. Comparison with 0.12 percent chlorhexidine was evaluated with the Mann-Whitney U test. A 5 percent significance level was adopted. Results: A total 11 constituents were identified in the essential oil. The main chemical components were 3-Heptadecene, (Z)- (36.13 percent), 1-Tridecene (14.7 percentand 1,8-cineole (9.72 percent). MIC of the essential oil was 36.195 mg/ml, whereas MBC was 39.114 mg/ml. The inhibition haloes of the experimental P. gingivalis gel were 25.533 mm ± 0.960 mm. Statistically significant differences were observed versus 0.12 percent chlorhexidine (23.282 ± 0.345) (p < 0.05). Conclusions: The 4.46 percent experimental gel composed of Eucalyptus globulus Labill essential oil displayed considerable antibacterial activity against Porphyromonas gingivalis ATCC 33277(AU)

Evaluation of the effect of coating vertex denture lining material with plant oils on microbial growth and hardness

Aims: To evaluate the effect of surface coating with natural plant oils (Salvia officinalis, ginger and eucalyptus) on Candida growth and the hardness of Vertex denture lining material. Materials and method: Forty five specimens were prepared from soft acrylic lining material, twenty five of which were 10x10x2mm in size for testing antifungal activity, and twenty samples were 20mm in diameter and 12mm in thickness, for testing shore A hardness after coating samples with three types of natural oils (Salvia officinalis, ginger and eucalyptus oils). Significant differences among the groups at (p≤0.05) level of significance were determined statistically with one way analysis of variance and Duncan's multiple range test Result: Antifungal assay showed a significant difference between five groups regarding Candida albicans growth (p≤ 0.05). For the hardness test, comparing different times of storage in water (1, 7, 14, 30 days) revealed a significant difference within all groups (p≤0. 05). While comparing the groups coated with natural oils with the control group, significant differences were found between different times of storage in water (1, 7, 30 day) (p≤0.05), except at 14 days of water storage there was no significant difference between groups (p>0.05). Conclusion: All tested natural oils were effective as fungicidal agents and increased the softness and duration of soft acrylic lining material.

In vivo transdermal permeation-enhancement activity and skin irritation of eucalyptus oil by using microdialysis technique / 中草药

Objective To evaluate the skin irritation of eucalyptus oil and its in vivo transdermal penetration enhancement properties by using the cutaneous microdialysis technique. Methods The CCK-8 assay was used to measure the toxicity of eucalyptus oil on HaCaT cells, and the TEWL values of the rat skin was determined to investigate the effect of eucalyptus oil on the skin integrity and irritation. Ligustrazine and geniposide were chosen as lipophilic and hydrophilic model drugs, respectively, and their microdialysis probe in vivo recoveries were determined using the retrodialysis method. After treatment with eucalyptus oil, the skin pharmacodynamics behaviors of two model drugs were investigated to evaluate its penetration-enhancement activity. Results The cytotoxicity test revealed there IC50 value of eucalyptus oil to HaCaT cells was 2.452 mmol/L, which was significantly higher than that of chemical penetration enhancer Azone (IC50, 0.266 mmol/L). Meanwhile, the eucalyptus oil had a certain impact on the rat skin TEWL values, but it was weaker than Azone, this implied that the eucalyptus oil had a mild skin irritation. The in vivo transdermal microdialysis tests revealed that the enhancement ratios (ER) of ligustrazine and geniposide were 11.40 and 13.79, respectively, indicating that eucalyptus oil could effectively facilitate the transdermal permeation of both of lipophilic and hydrophilic drugs. Although the penetration enhancement property was generally weaker than Azone, the ER value of eucalyptus oil was closely approximate to Azone. Conclusion The eucalyptus oil could promote the transdermal permeation of both lipophilic and hydrophilic drugs with mild skin irritation, which provided the data support for its application in topical preparation.

Determination of Eucalyptol, Camphor and Menthol in Compound Menthol Camphor Eucalyptus Oil Solution by GC / 中国药师

Objective: To establish a method for the determination of eucalyptol, camphor and menthol in compound menthol camphor eucalyptus oil solution by GC.Methods: An HP-INNOWAX 19091N-216 capillary column(60 m× 0.32 mm , 0.50 μm)was used.The carrier gas was nitrogen with the flow rate of 30 ml·min-1 , the gas was hydrogen with the flow rate of 40 ml·min-1 and the oxidant gas was air with the flow rate of 400 ml· min-1.The detector was FID and the inlet temperature was 250℃.The temperature program was as follows: the initial column temperature was 50℃, and then risen to 160℃ with a rate of 10℃·min-1 and kept for 5 min, and finally risen to 220℃ with a rate of 20℃·min-1 and kept for 3 min.The split ratio was 15∶1 and the injection volume was 1 μl.Results: The linear range of eucalyptol, camphor and menthol was 0.031 9-2.550 0 mg·ml-1 (r=1.000 0), 0.041 3-3.305 0 mg·ml-1 (r=1.000 0) and 0.053 7-4.294 0 mg·ml-1 (r=1.000 0), respectively.The average recovery was 98.24% (RSD=0.3% , n =9), 98.97% (RSD=0.4% , n =9) and 98.98% (RSD=0.5% , n =9), respectively.Conclusion: The method is sensitive and accurate with good stability, which can be used to determine the contents of eucalyptol, camphor and menthol in compound menthol camphor eucalyptus oil solution.

Irrational use of Eucalyptus oil in dentistry: a case report.

Use of herbal medicaments for dental pain is a practice still followed in some parts of rural India. Most often these herbal medicines are readily available to the rural without the prescription from an authorized practitioner. Eucalyptus oil is one such herbal drug which is widely used for a number of ailments. An unusual and a rare case of chemical injury secondary to the use of eucalyptus oil has been presented here. We have also described the management of the injury with herbal medication. This case report tends to highlights the dangers of self medication and also stresses on the role of herbal medications in dentistry.

Avaliação in vitro da atividade antifúngica de extratos de plantas e óleo de eucalipto sobre Trichophyton mentagrophytes / In vitro evaluation of the antifungal activity of plant extracts and eucalyptus oil on Trichophyton mentagrophytes

O presente estudo teve como objetivo determinar a ação antifúngica de extratos de plantas medicinais e óleo de eucalipto frente ao dermatófito Trichophyton mentagropytes, visando a utilização da fitoterapia no controle. As plantas utilizadas na obtenção dos extratos foram arruda (Ruta graveolens), citronela (Cymbopogon nardus), cravo de defunto (Tagetes minuta), eucalipto (Eucalyptus spp), graviola (Annona muricata), fruta do conde (Annona spp), manga (Mangifera indica), romã (Punica granatum), flores e folhas de primavera (Bougainvillea spectabilis). Verificou-se que uso de 0,5 por cento óleo de eucalipto no combate ao T. mentagropytes foi eficaz, já os extratos de citronela (4 por cento) eucalipto (5 por cento) e romã (8 por cento) atuaram como fungistáticos e os restantes não devem ser usados contra este dermatófito porque não causaram nenhum efeito.

The aim of this study was to assess the antifungal action of medicinal plant extracts and eucalyptus oil against the dermatophyte Trichophyton mentagrophytes in order to employ phytotherapy for its control. The plants used for extract production were common rue (Ruta graveolens), citronella (Cymbopogon nardus), wild marigold (Tagetes minuta), eucalyptus (Eucalyptus spp), sweetsop (Annona muricata), custard apple (Annona spp), mango (Mangifera indica), pomegranate (Punica granatum), besides flowers and leaves of bougainvillea (Bougainvillea spectabilis). The use of 0.5 percent eucalyptus oil was effective in controlling Trichophyton mentagrophytes; however, citronella (4 percent), eucalyptus (5 percent) and pomegranate (8 percent) extracts acted as fungistatic, and the remaining extracts should not be used against this dermatophyte since they did not have any effect.

Antifungal Activity of the Essential oil from Eucalyptus and Eucapytol against Candida albicans / 대한의진균학회지

BACKGROUND: Candida albicans is a member of the normal flora of the skin, mucous membranes and gastrointestinal tract. Vaginal candidiasis remains a significant problem in women OBJECTIVE: The purpose of this study was to develop the antifungal agent from the medicinal herbs traditionally used in Korea. Method: In this study, the essential oil of eucalyptus, cedar wood, clary sage, orange sweet and eucapytol were examined for antifungal activities against C. albicans. RESULTS: 1. Eucalyptus essential oil and eucapytol showed antifungal activity against C. albicans. 2. Minimal inhibitory concentration (MIC) of Eucapytol against eight strains and standard strains was 12.5 microliter/ml and 6.25 microliter/ml against two strains. 3. MIC of eucapytol was 25 microliter/ml against eight strains and 50 microliter/ml against two. CONCLUSION: Eucalyptus essential oil and eucalytol could be a candidate for a new antifungal agent for candidiasis and other fungal diseases.

Antifungal Activity of the Essential oil from Eucalyptus and Eucapytol against Candida albicans / 대한의진균학회지

BACKGROUND: Candida albicans is a member of the normal flora of the skin, mucous membranes and gastrointestinal tract. Vaginal candidiasis remains a significant problem in women OBJECTIVE: The purpose of this study was to develop the antifungal agent from the medicinal herbs traditionally used in Korea. Method: In this study, the essential oil of eucalyptus, cedar wood, clary sage, orange sweet and eucapytol were examined for antifungal activities against C. albicans. RESULTS: 1. Eucalyptus essential oil and eucapytol showed antifungal activity against C. albicans. 2. Minimal inhibitory concentration (MIC) of Eucapytol against eight strains and standard strains was 12.5 microliter/ml and 6.25 microliter/ml against two strains. 3. MIC of eucapytol was 25 microliter/ml against eight strains and 50 microliter/ml against two. CONCLUSION: Eucalyptus essential oil and eucalytol could be a candidate for a new antifungal agent for candidiasis and other fungal diseases.

Disinfection Efficacy of Lemon Eucalyptus Oil on Indoor Air / 中华医院感染学杂志

OBJECTIVE To observe the efficacy of lemon eucalyptus oil on disinfection of indoor air.METHODS Fumigating method and air sampling method were used to evaluate the disinfection efficacy.Ultraviolet disinfection method was used as control.RESULTS Aerosol spraying disinfection with the lemon eucalyptus oil at a dosage of 10 ml/m3 followed by closing the room for 30 min can killed 95.4% of the natural bacteria of indoor air.Irradiation by 30 W ultraviolet lamp for 30 min under same conditions killed 89.7% of the natural bacteria of indoor air.CONCLUSIONS The efficacy of the lemon eucalyptus oil disinfectant at 10ml/m3 concentration in killing natural bacteria in indoor air fulfils the requirement of eligible disinfection and is better than the disinfection efficacy of ultraviolet irradiation.

Preparation and Appraising of Eucalyptus Oil?-cyclodextrin Inclusion Compound / 中国药房

OBJECTIVE:To prepare and appraise eucalyptus oil?-cyclodextrin inclusion compound,and to testify the feasibility of transforming the dosage form of eucalyptus oil by clathration techniques.METHODS:The physicochemical properties of inclusion compound was identified by thin-layer chromatography(TLC),infrared spectroscopy(IR),ultra-violet spectroscopy(UV)and gas chromatograph mass spectrometer(GC-MS),respectively.Meanwhile,the changes in constituents and clathration outcomes of eucalyptus oil before and after clathration were also investigated.RESULTS:The analytic result of TLC,IR and UV showed that stable inclusion compound has been formed from eucalyptus oil and?-cy?clodextrin.The result of GC-MS demonstrated that there was no significant change in the essential components and the percentage composition of eucalyptus oil before and after inclusion.CONCLUSION:Stable inclusion compound can be made from eucalyptus oil and?-cyclodextrin meanwhile without changes in main components and the percentage composition of eucalyptus oil.