Preparation of low molecular weight Eucheuma striatum sulfated polysaccharides by oxidative degradation with H_2O_2 under the ultrasonic wave / 中国海洋药物

Objective To prepare low molecular weight Eucheuma striatum sulfated polysaccharides. Methods Eucheuma striatum sulfated polysaccharides were degraded with hydrogen peroxide under the ultrasonic wave. Molecular weight and the content of sulfate radical, 3,6-anhydro-galactose of all sulfated polysaccharides were determined. Results The polysaccharides with molecular weight from 5000 to 40000 were prepared and the content of sulfate radical was above 18.5%. Conclusion This method is fit for preparing low molecular weight and good water-solubility polysaccharides. The post-treatment is simple and the method keeps the sulfated radical perfectly.

Antiviral activities of polysaccharides from Eucheuma gelatinae and Eucheuma striatum in vitro / 中国海洋药物

Objective The antiviral effects of polysaccharides from Eucheuma gelatinae and Eucheuma striatum on herpes simplex virus type 1(HSV-1) and coxsackie virus(CVB3) in vitro were assayed and their antiviral mechanism was elucidated.Methods The antiviral effects of samples were evaluated with cell cytotoxicity by MTT and cytopathic effect(CPE) methods.Antiviral mechanism of two extracts was investigated by four different ways.Results and Conclusion Polysaccharides from Eucheuma gelatinae and Eucheuma striatum had an obviously HSV-1 and CVB3 inhibitory effect.Antiviral activities of two extracts(ESPA and ESPA2) were better than acyclovir(ACV).Antiviral activities of all extracts were better than ribavirin injection.Polysaccharides from Eucheuma striatum had remarkable anti-CVB3 effects.The experiment also showed that Eucheuma polysaccharides not only kill the above viruses directly but also restrain them via getting into cells or absorbing on cells.