RESUMO
Objective To evaluate the safety of the Eucommia folium and to provide the basis of the rational use ofEucommia folium resource.Methods According to < Technical Standards for Testing & Assessment of Health Food>,version 2003, acute safety test/micronucleus test of born marrow in mice/sperm shape abnormality test in mice/Ames test andthirty-day feeding test were all performed after water extract concentrations of Eucommia folium treatment (1 mLconcentrations equal to 1.5 g Eucommia folium) . Results On the acute toxicity test, the MTD of rats and mice were both40.0 mL/kgbw. The results of genetic toxicity test were all negative in the dosage of 10 g/kg bw(according to the dosage ofEucommia folium) .The results of thirty-day feeding test indicated that there have no significant differences in body weight,blood cytology indexes, blood biochemical indexes, organ/body ratio and pathematology examination in the rats in all thegroups (0.83, 1.67, 3.30 mL/kg) as compared to the control group(P>0.05) . Conclusion Under this experimentalcondition, no obvious abnormity and toxic reaction were observed.
RESUMO
Objective To determine the four effective components in Eucommia folium. Methods HPLC was employed to determine the contents of four effective components: chlorogenic acid, quercetin, kaempferol, rutin. The chromatogram colume was WondaSil C18 Superb (4.6 mm × 250 mm, 5 µm), gradiently eluting with methanol as mobile phase A and 0.4% phosphoric acid as phase B at a flow rate of 1 ml/min. The injection volumn was 10 µl. Detection wavelength was set at 262 nm. Results Chlorogenic acid was linear in the range of 0.825-4.123 µg with correlation coefficient of 0.9999. The average recovery was 101.89% with RSD 0.49% (n=6). Quercetin was linear in the range of 0.165-0.824 µg with correlation coefficient of 0.9998. The average recovery was 99.88% with RSD 2.64%. Kaempferol was linear in the range of 0.021-0.104 µg with correlation coefficient of 0.9999. The average recovery was 100.0% with RSD 0.89%. Rutin was linear in the range of 0.002-0.012 µg with correlation coefficient of 0.9991. The average recovery was 100.0% with RSD 0%. Conclusion The method is convenient, fast, accurate and practicable. It is suitable for content determination of components in Eucommia folium.