Research on Q-markers of Eupatorium lindleyanum based on analytic hierarchy process-entropy weight method and network pharmacology / 中国中药杂志

The potential quality markers( Q-markers) of Eupatorium lindleyanum were studied with analytic hierarchy process(AHP)-entropy weight method(EWM) and network pharmacological method. Based on the concept of Q-markers of traditional Chinese medicine, AHP-EWM was employed to quantitatively identify the Q-markers of E. lindleyanum. AHP method was applied to the weight analysis of the validity, testability, and specificity of the first-level indexes, and EWM method was used to analyze the secondlevel indexes supported by literature and experimental data. At the same time, based on the theory and method of network pharmacology, the component-target-disease-efficacy network of E. lindleyanum was built, and the components most closely related to the efficacy of resolving phlegm and relieving cough and asthma were screened out. Through the integrated analysis of the results obtained with AHP-EWM and network pharmacological method, 13 compounds including rutin, quercetin, nepetin, cirsiliol, luteolin, hyperoside,isoquercitrin, kaempferol, caffeic acid, eupalinolide K, eupalinolide A, eupalinolide B, and eupalinolide C were comprehensively identified as the potential Q-markers of E. lindleyanum. The results provide a basis for the quality control of E. lindleyanum.

Chemical constituents from ethyl acetate extract of Eupatorium adenophorum / 中草药

Objective: To study the chemical constituents of the Eupatorium adenophorum. Methods: The chemical constituents were isolated and purified by silica gel chromatography repeatedly from the ethyl acetate extract of E. adenophorum, and their structures were identified by spectral analysis and chemical methods. Results: Fourteen compounds were isolated from E. adenophorum and identified as p-hydroxybenzoic acid ethyl ester (1), (1R,4R)-8aα-hydroxy-1-isopropyl-4,7-dimethyl- 1,2,3,4,6,8a-hexahydro-naphthalene-2,6-dione (2), daedalin A (3), 10-oxo-7-hydroxy-nordehydrotremetone (4), caffeoyl acetate (5), syringic acid (6), 3-hydroxy-4-(1-oxo-ethane)-benzoic acid (7), ferulic acid (8), p-hydroxyphenylethyl alcohol (9), protocatechuic acid ethyl ester (10), 4-hydroxy-3-isopropyl benzoic acid (11), balanophonin (12), indole-3-carboxylic acid (13), and 6-methoxy kaempferol (14). Conclusion: Compound 11 is obtained from natural products for the first time, compounds 1, 3, 6, 7, 9, 10, 12-14 are obtained from this genus for the first time, and compound 5 is obtained from this plant for the first time.

Analysis of chemical constituents in Eupatorium lindleyanum by UPLC-Q-TOF/MS / 中草药

Objective: To analyze and identify the chemical constituents from Lindley eupatorium by using UPLC-Q-TOF/MS. Methods: The separation was performed on Waters Acquity UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm) column with gradient elution of 0.1% formic acid (A)-acetonitrile (B), the flow rate was 0.2 mL/min. The column temperature was set at 35 ℃. The MS analysis was based on information associated mode (IDA), and positive and negative ions were collected respectively. Results: A total of 26 compounds in L. eupatorium were identified by PeakView, combined with the mass spectrometry data of each chromatographic peak in the database, and the cleavage law of secondary fragment of each peak of which11 compounds were first reported for L. eupatorium. The main chemical constituents included flavonoids, nucleosides, alkaloids, phenylpropanoid, sesquiterpenoids, coumarins, polyols, etc. Conclusion: The method is accurate, reliable and effficient, which is suitable for rapid identification of ingredients in L. eupatorium, which provides a reference for clarify its efficacy and material basis.

A new cadinane-type sesquiterpenoid from Eupatorium adenophorum Spreng / 药学学报

Four cadinane-type sesquiterpenes were obtained from the petroleum ether of 95% ethanol extract of Eupatorium adenophorum Spreng by using an HP-20 macroporous resin column, silica gel, and semi-preparative HPLC. Their structures were determined by physical, chemical and spectroscopic methods and identified as eupatorinol (1), (+)-(5R,7S,9R,10S)-2-oxocadinan-3,6(11)-dien-12,7-olide (2), (1S,4R)-7-hydroxycalamenen-3-one(3) and (-)-(5R,6R,7S,9R,10S)-cadinan-3-ene-6,7-diol (4). Among them, compound 1 is a new cadinane-type sesquiterpene, and compound 3 was isolated from this genus for the first time. In bioassay, none of these compounds displayed obvious cytotoxicity.

Chemical constituents from roots of Eupatorium chinense / 中草药

Objective To study chemical constituents of the roots of Eupatorium chinense. Methods The chemical constituents were separated and purified by the normal phase silica gel column chromatography, preparative thin-layer chromatography, and preparative HPLC. Their structures were determined by various spectral data. Results Nineteen compounds were isolated from the acetic ether extract of E. chinense and the structures were identified as euparin (1), 1-[2-(1-acetoxymethyl-vinyl)-6-hydroxy-benzofuran-5-yl]- ethanone (2), 6-hydroxy-3β-methoxytrematone (3), euparone (4), 8-methoxy-9-hydroxythymol (5), dehydroespeleton (6), 8-methoxy-9-hydroxythymol 3-O-angelate (7), 9-hydroxythymol (8), 4-hydroxy cinnamic acid methyl ester (9), p-hydroxy benzaldehyde (10), 8,9-dehydro-10-hydroxythymol (11), diisobutyl phthalate (12), dibutyl phthalate (13), p-coumaric acid (14), dihydrocoumarin (15), methylcaffeate (16), 2,5-dimethylphenol (17), 1H-indazole (18), and (Z)-3-(hydroxymethyl)-7-methylocta- 2,6-dienly1 acetate (19). Conclusion The chemical constituents are investigated and identified from the roots of E. chinense for the first time. Among them, compounds 2, 3, 5-11 are isolated from E. chinense for the first time, and compounds 6, 9, 12, 14-19 are isolated from the genus of Eupatorium for the first time. Compounds 1-4 are benzofurans, which are the characteristic constituents in Eupatorium genus.

Optimization of Eehanol Extraction Technology of Anti-atherosclerosis Effective Fraction in Lindley eupatorium by Multiple Index Scoring Method / 中国药房

OBJECTIVE:To optimize the ethanol extraction process of anti-atherosclerosis effective fraction of Lindley eupatorium. METHODS:Using the contents of hyperin,quercetin,kaempferol and jaceosidin as indexes,the effects of ethanol volume fraction,liquid-solid ratio,extraction time and extraction times on ethanol extraction technology were investigated. By multiple index scoring method,orthogonal test was designed to optimize extraction technology,and validation test was conducted. RESULTS:The optimal extraction technology was 70% ethanol as extraction solvent with solid-liquid ratio of 12 : 1,reflux extracting for 3 times,2 h each time. Results of 3 batches of validation tests showed that the contents of 4 marker components were in high level,being 5.305 3,1.560 1,1.986 5,6.703 6 mg/g in average(RSD<1.1%,n=3). Results of validation tests were stable and reliable,and were in good agreement with the results of orthogonal tests. CONCLUSIONS:The optimized ethanol reflux extraction technology of L. eupatorium is stable and reliable,and performs well. Energy consumption and cost are suitable for industrial production.The results provide technology basis for further purification and enrichment of anti-atherosclerosis effective components of L.eupatorium.

Effect and mechanism of methanolic extract of Eupatorium to model rats with chronic muscle injury / 中成药

AIM To investigate the effect and mechanism of methanolic extract of Eupatorium (MEOE) to model rats with chronic soft tissue injury.METHODS The model rats were established by mechanical injury and a subsequent two-week normal feeding for respective administration of high,medium and small dosage of MEOE once a day successively for 14 days.An array of indices,the level of superoxide dismutase (SOD),malondialdehyde (MDA),prostaglandin E2 (PGE2),nitric oxide (NO),interleukin-6 (IL-6) and histamine,the expression of tumor necrosis factor-alpha (TNF-α),nitric oxide (NO) and Collagen-Ⅰ/Ⅲ,and the activity of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were measured to analyze the effect of MEOE to model rats with chronic muscle injury.RESULTS MEOE resulted in apparent reduction of contents of MDA,PGE2 and NO,and the levels of TNF-α and IL-6 in muscular tissue (P ＜ 0.05),significantly increased of the SOD in muscular tissue (P ＜ 0.01),a remarkably inhibited expression of the tissue Collagen-Ⅰ/Ⅲ protein (P ＜ 0.01),and significantly improved activity of tissue VEGF and bFGF (P ＜ 0.01).CONCLUSION The certain therapeutic effects of MEOE to rats with chronic muscle injury may correlate with its influence to the levels of inflammatory factors inhibition,the oxidative stress relief,the overexpression of collagen-Ⅰ/Ⅲ inhibition,the VEGF and bFGF activity improvement,and the time spare from the repairing.

Study on the Antihypertensive Effects of Water Decoction of Eupatorium lindleyanum on Spontaneously Hy-pertensive Rats and Corresponding Mechanism / 中国药房

OBJECTIVE:To study the antihypertensive effects of water decoction of Eupatorium lindleyanum on spontaneously hypertensive rats (SHR) and corresponding mechanism. METHODS:50 SHR were randomly divided into model group,captopril tablets group(positive drug,10 mg/g)and the groups of high,medium and low-dose(65.45,32.73,16.36 g/kg)water decoction of E. lindleyanum,with 10 rats in each group. The rats in the model group were given normal saline ig,while those in other groups were given corresponding drugs ig,once a day,for 7 consecutive weeks. The systolic blood pressure (SBP) and diastolic blood pressure(DBP)of the caudal arteries of the rats were measured every week,and the dynamic changes in the blood pressure of the rats as a result of drug intervention were recorded. The rats were sacrificed 7 weeks later,and the levels of endothelin(ET), angiotensin (AngⅡ) and nitric oxide (NO) in serum were determined. RESULTS:After 7 weeks of drug use,compared to the model group,the rats in the drug administration groups had significantly lower SBP and DBP of the caudal artery(P<0.05),show-ing obvious dose-effect and time-effect relationships in the antihypertensive effect of water decoction of E. lindleyanum,and had ob-viously lower levels of NO and AngⅡ and markedly higher level of ET in serum,demonstrating a dose-effect relationship. CON-CLUSIONS:The water decoction of E. lindleyanum has an obvious antihypertensive effect by a mechanism which may be related to decrease in the levels of NO and AngⅡin serum and increase in the level of ET therein.

Screening of various leaf extracts of Chromolaena odorata L. for biochemical constituents and antimicrobial sensitivity.

Background: Ethanolic, aqueous, petroleum ether and ethyl acetate extracts of leaves of Chromolaena odorata were studied for their antimicrobial activity. Twelve bacterial species, including six Gram-positive and six Gram-negative bacteria viz. Vibrio cholerae, Shigella sonnei, Klebsiella pneumoniae, Salmonella paratyphi A, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus citreus, methicillin-resistant S. aureus, nonpathogenic Mycobacterium gordonae and Mycobacterium fortuitum along with fungi Candida albicans and Aspergillus niger were studied. Methods: This study involved antibiotic sensitivity testing of extracts against a wide spectrum of micro-organisms, to determine the antimicrobial activity of the plant. Results: Initial phytochemical testing unveiled the chemical constituents of C. odorata as saponins, flavonoids, tannins, steroids and proteins. The zone of inhibition for the respective extracts was compared with the zone of the standard antibiotic and was found to be quite effective. Conclusions: The ethanolic extract of C. odorata exhibited significant antimicrobial activity. The plant extracts could be used to treat resistant form of prevailing infections. Successful antimicrobial drugs can be developed out of these extracts if specific compounds are isolated and purified.

Antipyretic Effects of Eupatorium chinense and Its Mechanism / 中草药·英文版

Objective: To investigate the antipyretic effect of Eupatorium chinense and its mechanism. Methods: The content of arginine vasopressin (AVP) in ventral septal area (VSA) and blood plasma, and cyclic adenosine monophosphate (cAMP) levels of hypothalamus and blood plasma were determined by enzyme linked immunosorbent assay. Results: The body temperature (Tb) was decreased at 1 h after administration of E. chinense (3 and 6 g/kg) and Aspirin (0.3 g/kg) respectively, which was significantly different from the temperature of fever model group. The antipyretic effect of Aspirin and E. chinense lasted for longer time. Aspirin (0.3 g/kg) and E. chinense (3 and 6 g/kg) reduced the level of cAMP in hypothalamus of fever rats and increased AVP content in plasma. The changes in cAMP content in plasma of all drug treatment groups were not obvious. Conclusion: E. chinense has strong antipyretic effect and may affect the production of AVP and cAMP in fever rats.

Study analgesic effect site of eupatorium odoratum L and its mechanism / 重庆医学

Objective To study the analgesic effect of Eupatorium odoratum L and its mechanism .Methods Mice were random‐ly divided into 6 groups .Swinging tail method in spinal cord destroyed mouse ,hot‐plate and twisted body method in naloxone blocked mouse ,and formalin‐induce inflammatory pain method were done to investigate the analgesic effect of Eupatorium odoratum L .Each pain index under each experiment condition was determined after intra gastric administration of each group of mice .The concentration of NO and PGE2 in serum and brain were measured to study its mechanisms .Results High‐dose of Eupatorium odor‐atum L significantly enhanced pain threshold in either spinal cord destroyed or naloxone blocked mouse with pain induced by hot and acetic acid(P<0 .05) .High and middle dosage groups of Eupatorium odoratum L markedly reduced the first‐and second‐phase re‐sponses of formalin‐induced inflammatory pain mouse(P< 0 .05) ,and decreased the writhing times and licking rear feet times caused by twisted body method(P<0 .05) .The level of NO and PGE2 in serum and brain of mouse induced by formalin were both decreased(P<0 .05) .Conclusion Eupatorium odoratum L has notable analgesic effect ,which may be related to reduce the level of NO and PGE2 in central and peripheral site .

Study on the Flavonoids in She Medicine Eupatorium chinense / 中国药房

OBJECTIVE:To study the flavonoids in She medicine Eupatorium chinense. METHODS:Silica gel,ODS and Sep-hadex LH-20 column chromatography were conducted to isolate and purify the flavonoids in She medicine E. chinense,and com-pound structures were analyzed and identified based on the physicochemical properties and spectral data. RESULTS:From the ethyl acetate extract of E. chinense,10 flavonoids were isolated as tricin (1),quercetin(2),kaempferol(3),luteolin(4),luteo-lin-7-O-β-D-glucoside (5),4-methoxyctricin (6),quercetin-3-O-β-D-glucoside(7),kaempferol-3-O-β-D-glucoside(8),kaempfer-ol-3-O-rutinoside(9)and rutin(10). CONCLUSIONS:Compound 1-10 are isolated from E. chinense for the first time. The study provides certain basis for the quality evaluation of E. chinense.

The Anticonvulsant Effect of Eupatorium Birmanicum DC Leave Extract Alone and in Combination with Phenytoin Against MES Seizure in Albino Mice.

To evaluate the anticonvulsant activity of aqueous extract of Eupatorium birmanicum DC leave (EB) alone and in combination with phenytoin against MES seizure in albino mice. Method: Aqueous extract of EB was prepared using Soxhlet apparatus. The anticonvulsant effect of the extract was tested on prescreened albino mice at 3 doses (200, 400 & 800 mg/kg). After 1 hr of oral administration of EB the animals were subjected to MES seizures by convulsiometer with a current of 45 mA for 0.2 sec via transauricular electrodes and the duration of the THLE was recorded. Sub-anticonvulsant dose of phenytoin was also determined and the effect of its combination with the most effective dose of EB tested. Results: EB aqueous extract exhibited significant anticonvulsant activity in the MES model at doses 400 mg/ kg (p<0.01) & 800 mg/kg (p<0.001). This reduction in the duration of THLE at 800mg/kg of EB was further reduced significantly (p<0.001) when combined with subanticonvulsant doses of phenytoin (10mg/kg). Conclusion: The aqueous extract of E. birmanicum leaves showed significant anticonvulsant activity in MES seizure model in albino mice and it significantly increased the anticonvulsant effect of phenytoin in the same animal model.

The inhibitory effect of eupatorium japonicum thunb and foeniculum vulgare extract on prostatic hyperpla-sia in rats / 医学研究生学报

Objective Benign prostatic hyperplasia ( BPH) is one of common diseases in aged males , and searching for new therapeutic drugs to BPH has been a research hotspot in recent years .This article was to study the inhibitory effect of eupatorium ja-ponicum thunb and foeniculum vulgare extract ( EFE) on benign prostatic hyperplasia in rats and its possible mechanism . Methods 48 male rats were randomly divided into 6 groups:normal control group without any treatment , model group of BPH treated with subcu-taneous injection of testosterone propionate , positive control group of BPH treated with dutasteride , high, middle and low dosage groups according to different EFE dosage (156 mg/kg, 234 mg/kg and 312 mg/kg).45 days after the treatment, the rats were sacrificed for measurement of the prostate glandular wet weight , the index of prostate gland ( PI ) , the morphological changes of prostate gland by light microscopy and the content of sex hormone . Results The prostate wet weight and PI decreased after EFE treatment for 45 days compared with the BPH model group(P<0.01 ).The hyperplastic glandular epithelium papilla waned and even disappeared in three EFE groups under the light microscope , and the epithelial cells became cubical or flat .High dosage EFE group (312 mg/kg) has simi-lar efficacy to dutasteride group .EFE significantly reduced serum testosterone content , dihydrotestosterone content and T/E2 ratio( P<0.05 ). Conclusion EFE can significantly inhibit prostatic hyperplasia in rats , and its mechanism is related to the decrease of the contents of serum testosterone and dihydrotestosterone as well as T/E2 ratio.

Study on larvicidal activity of weed extracts against Spodoptera litura.

Larvicidal activity of various concentrations of crude aqueous leaf extracts of E. triplinerve were studied against S. litura following the treatment on fourth instar larvae. The results of the total percent mortality (from fourth instar to adult) demonstrated that the mortality rate progressively increased (41.42, 58.57, 69.80, 84.49, 86.77, 90.55, 91.30, 90.54) as the extract concentrations increased (1, 2, 3, 5, 7.5, 12.5, 15, 17.5%). The maximum of 92 % total mortality was observed at 15 % concentration of the extract. It was significant to note that the percent mortality observed from 5 % onwards was more than 85 %. Results of adult emergence inhibition indicated that the EI50 of E triplinerve was more effective at 4.07 %. Interestingly the calculated EI90 value was 14.10 %. This is an ideal eco-friendly approach for the control of agricultural pest, S. litura.

Composição química volátil e não-volátil de Eupatorium ballotifolium Kunth, Asteraceae

Este trabalho descreve a composição química dos óleos essenciais e o isolamento de onze substâncias de Eupatorium ballotifolium Kunth, Asteraceae. Os óleos essenciais foram obtidos por hidrodestilação, analisados por CG-EM e avaliados quanto às suas atividades frente à enzima acetilcolinesterase. O rendimento dos óleos foi de 0,11 por cento para as folhas e 0,03 por cento para os talos. Os principais constituintes dos óleos foram os sesquiterpenos β-cariofileno (24,9 e 22,2 por cento), espatulenol (17,7 e 12,4 por cento) e epóxi-allo-aromadendreno (23,0 e 23,6 por cento). Do extrato hexânico da parte aérea foi isolada a mistura de β-sitosterol e estigmasterol, incluindo suas formas glicosiladas, e os triterpenos acetato de taraxasterila e taraxasterol, enquanto, do extrato etanólico foram isolados os flavonóides nepetina and 3-O-glicosil-quercetina. Do extrato hexânico das raízes foram isolados os triterpenos epi-friedelanol e damara-20,24-dien-3β-ol e do extrato etanólico a cumarina 11-hidroxi-11,12-di-hidroobliquina. As estruturas de todos os compostos foram determinadas usando técnicas espectroscópica tais como IV, EM e RMN ¹H e 13C.

This work describes the chemical composition of the essential oils and the isolation of eleven substances from Eupatorium ballotifolium Kunth, Asteraceae. The essential oils were obtained by hydrodistillation, analyzed by GC/MS and evaluated towards the acetylcholinesterase enzyme. The oils yield was of 0.11 percent for the leaves and 0.03 percent for the stems. The main constituents of the oils were the sesquiterpenes β-caryophyllene (24.9 and 22.2 percent), spathulenol (17.7 and 12.4 percent) and epoxy-allo-aromadendrene (23.0 and 23.6 percent). From the hexane extract of the aerial part were isolated a mixture of sitosterol and stigmasterol, its glucosides, and the triterpenes taraxasteryl acetate and taraxasterol, while from the ethanol extract were obtained the flavonoids nepetin and 3-O-glucoside-quercetin. The triterpenes epi-friedelanol and dammara-20,24-dien-3β-ol were obtained from the hexane extract of roots, while the coumarin 11-hydroxy-11,12-di-hydroobliquine was obtained from the ethanol extract. The structures of all compounds were determinate based on spectroscopic methods such as IR, MS and ¹H and 13C NMR.

A new species of Hyperomyzus (Neonasonovia) (Hemiptera: Aphididae) from Jamaica / Una Nueva Especie de Hyperomyzus (Neonasonovia) (Hemiptera: Aphididae) de Jamaica

Esta descripción está basada en ejemplares ápteros colectados en Jamaica sobre Eupatorium sp. (Asteraceae). Se proveen fotografías e ilustraciones de la nueva especie. La nueva especie es comparada con una especie no descrita de Costa Rica. Se diferencia de Hyperomyzus (Neonasonovia) nigra (Baker) por tener siphunculi más estrechos, pocas rhinarias secundarias y el rostro más alargado.

Hyperomyzus (Neonasonovia) pullatus Hall & Garraway sp. n (Hemiptera: Aphididae) is described based on apterous specimens collected from Eupatorium sp., (Asteraceae) in Jamaica. Photos and illustrations are provided. It is compared with an undescribed species from Costa Rica. It is distinguished from Hyperomyzus (Neonasonovia) nigra (Baker) by having narrower siphunculi, less secondary rhinaria and a longer rostrum.

Evaluación del efecto tóxico de extractos de Eupatorium microphyllum L.F. (Asteraceae) sobre larvas de Aedes aegypti (Diptera: Culicidae) en condiciones de laboratorio / Evaluation of the toxic effect from Eupatorium microphyllum L.F. (Asteraceae) extracts on Aedes aegypti larvae (Diptera: Culicidae) in laboratory conditions

En el presente trabajo se evaluó la actividad tóxica de extractos de Eupatorium microphyllum L.F. sobre larvas de IV estadio del mosquito Aedes aegypti (Linneaus), bajo condiciones de laboratorio. Se utilizaron extractos acuosos en concentraciones del 500 mg L-1, 1.500 mg L - 1 y 2.500 mg L-1 y acetónicos en concentraciones de 10 mg L-1, 20 mg L-1, 30 mg L-1, 40 mg L-1 y 50 mg L-1. Los bioensayos se realizaron por triplicado, cada uno con 20 larvas, expuestas durante 24 horas a 150 mL de solución. En todos los ensayos biológicos se emplearon grupos control. En la evaluación de los extractos acetónicos, se empleó un control negativo para evitar que la mortalidad de las larvas ocurriera a causa del solvente. Los extractos acuosos mostraron acción moderadamente baja en la mortalidad de larvas, menor del 20%. Por el contrario, la acción de los extractos acetónicos se observó a 10 y 20 mg L-1, con 15% de mortalidad, mientras que a 30 y 40 mg L-1 se registraron 22 al 38% de mortalidad, en tanto que a 50 mg L-1 la mortalidad fue del 95,4% con resultados estadísticos altamente significativos. Las concentraciones de los extractos acetónicos mostraron ser las más eficientes para el control de los mosquitos seleccionados. Ambos tipos de extractos mostraron efecto tóxico en larvas de A. aegypti ; sin embargo, se observó mayor efecto en los extractos acetónicos en relación con los extractos acuosos de E. microphyllum, lo cual constituye una alternativa viable en la búsqueda de nuevos larvicidas a partir de compuestos naturales.

In the present work the toxic activity of extracts of Eupatorium microphyllum L.F. was evaluated on 4 th instar larvae of the mosquito Aedes aegypti (Linneaus), under laboratory conditions. Aqueous extracts were utilized in concentrations of 500 mg L-1, 1,500 mg L-1 and 2,500 mg L-1 and acetone in concentrations of 10 mg L-1, 20 mg L-1, 30 mg L-1, 40 mg L-1 and 50 mg L-1. The bioassays were carried out for triplicate each one with 20 larvae, exposed for 24 hours to 150 mL of solution. In all the bioassays were employed control groups. In the evaluation of the acetone extracts, a negative control was employed to avoid that the mortality of the larvae to occur on account of the solvent. The Aqueous extracts showed low moderate action in the mortality of larvae, less than 20%. On the contrary, the action of the acetone extracts was observed to 10 and 20 mg L-1 with 15% of mortality, while to 30 and 40 mg L-1 were registered 22 to 38% of mortality. However, to 50 mg L-1 the mortality was of 95.4% with highly significant statistical results. The concentrations of the acetone extracts showed to be the most efficient for the control of the mosquitoes selected. Both types of extracts showed toxic effect in larvae of A. aegypti, nevertheless, greater effect in the acetone extracts was observed relating to the aqueous extracts of E. microphyllum, which constitutes a viable alternative in the search of new larvicides from composed natural.