Study on Antitumor Activity of Ethyl Acetate Extract of Euphorbia lunulata Bge / 世界科学技术-中医药现代化

The ethyl acetate extract of Euphorbia lunulata Bge was separated and researched on anticancer activity about it with in vitro cell experiments,in order to provide a theoretical basis for the exploit and utilization of E.lunulata and more options to develop natural anticancer drugs.Quercetin and Gallic Acid in ethyl acetate were extracted by HPLC,which the ethyl acetate extract of E.lunulata was separated and extracted by solvent system.The MTT was used to measure the inhibition of each component with different concentration from ethyl acetate extract to the 2R-75-30 breast cancer cell.The results showed that Quercetin and Galic Acid of ethyl acetate extract were 19.99％ and 55.04％,respectively.There were 17 component from the ethyl acetate extract,and finally merged into six fractions.Each component (the concentration of 50 μg/ml-150 μg/ml range) from ethyl acetate extract of E.lunulata could inhibit the growth of ZR-75-30 breast cancer cell.And a certain gradient relationship with the drug concentration increased with the inhibition rate enhancement on the 2R-75-30 breast cancer cell's growth.The 2nd fraction showed the highest activity.It was concluded that there were flavonoids and phenolic acids in ethyl acetate extract.Each component from the extract showed inhibition on the growth of breast cancer cell if it was further separated.This study was designed to provide a theoretical basis to exploit the anticancer drugs from E.lunulata.

Chemical constituents in whole herb of Euphorbia lunulata / 中草药

Objective: To study the chemical constituents in the whole herb of Euphorbia lunulata. Methods: The compounds were separated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the compound were identified on the basis of chemical and spectral methods. Results: Seventeen compounds were isolated from 90% ethanol extract from the whole herbs of E. lunulata and identified as 1-octacosanol (1), β-sitosterol (2), octadecyl caffeate (3), cycloart-23Z-en-3β, 25-diol (4), scopoletion (5), 24-methylenecycloartanol (6), stigmasterol (7), vifolin (8), o-phthalic acid bis-(2-ethyl decyl)-ester (9), ferulic acid hexacosyl ester (10), scopoletin-7-glucoside (11), β-amyrin (12), gallic acid (13), sucrose (14), 17-hxdroxyjolkinolide A (15), jolkinolide A (16), and jolkinolide B (17). Conclusion: Compounds 1,3,8-12 and 14-17 are isolated from this plant for the first time, and compound 9 is isolated from the plants of Euphorbia L. for the first time.

A new jatrophane type diterpene from Euphorbia lunulata / 中草药

Objective: To study the chemical constituents of Euphorbia lunulata. Methods: Separation and purification were performed on silica gel chromatography and HPLC, while their structures were identified on the basis of physicochemical characteristics and spectroscopic data. Results: A new compound was isolated from anhydrous ethanol extracts of E. lunulata. The compound was elucidated as 2α,3β,5α,9α,15β-pentaacetoxy-11,12-epoxy-7β-isobutyryl-8α-benzoyloxyjatropha-6(17)-en-4-one. Conclusion: Compound 1 is a new compound named 8Bz-esulatin A.