RESUMO
BACKGROUND: Longer RBC storage will benefit blood banker by reduced outdating and patients by receiving fewer nonviable cells and RBC breakdown products as well as by having the opportunity to participate in markedly improved autologous storage system. METHODS: We rejuvenated whole blood 10 units and RBC 15 units, on 1-3 days after expiration, then they were divided into four, washed, and suspended with four kinds of solutions containing saline adenine glucose, mannitol, and phosphate, in closed system. For the evaluation of RBCs, 30 min saline stability (SS), 2 h SS, % hemolysis, ATP, 2,3-DPG, P50, LDH, potassium, and plasma hemoglobin were tested at every week during 42 days. RESULTS: On 35th day, 30 min SS of RBCs was higher than 88% and % hemolysis of RBCs was lower than 1% in solutions containing phosphate. ATP of RBCs was the 50% of the reference value of healthy persons and 2,3-DPG of RBCs was relatively higher in solutions containing phosphate (SAGMP1, 2, 3) than in solution without phosphate (SAGM). CONCLUSION: We could successfully rejuvenate outdated RBCs and extend the expiration date to additive 35 days, but for practical use, post transfusion RBC survival and safety should be evaluated in the future.