The Study of Total Flavonoids in Leaves of Lonicera Hypoglauca Miq / 医学研究杂志

Objective To study the qualitative analysis and quantitative analysis of flavonoids and other ingredients in leaves of Lonicera hypoglauca miq,and to optimize the reflux extraction condition for total flavonoids from leaves of lonicera hypoglauca miq. Methods Preliminary test was used for qualitative analysis. UV-spectrophotometry was used to determinate content of total flavonoids. By orthogonal design,the extraction rate of flavonoids was determined in different factors of ethanol reflux extracting. Results Leaves of lonicera hypoglauca miq contained alkaloids and flavonoids.The optimum conditions of ethanol reflux extracting total flavonoids are 12 times 50% ethanol,and refluence for 1.0h at water bath for two times.According to validate experiment,the average total flavonoids content was 16.6% in leaves of lonicera hypoglauca miq,with RSD=1.52%(n=3). Conclusion Flavonoids are in higher level in leaves of Lonicera hypoglauca miq. There are some chemical similarity in leaves of lonicera hypoglauca miq and leaves of flos lonicerae.

Optimizing Extraction Technics for Paishi Granule by Orthogonal Test / 中国药房

OBJECTIVE:To optimize the extraction process of paishi granule.METHODS:The extraction process of paishi granule was optimized by L 9 (3 4 )orthogonal test with total flavonoids as index,and with duration of decoction,times of de?coction,the amount of water added and the amount of alcohol used for extraction as factors of investigation.RESULTS:The optimum extraction condition was the following,the amount of water added to which was12times that of the medical material,and it was decocted120min for3times,which then was dried and crushed,then60%alcohol,which was40times that of the amount of medicinal material,was extracted by refluxing for3times with30min each time,filtered while hot,and the alcohol was recovered.The yield of extractum and the content of the total flavonoids were fairly high,and there was a good repro?ducibility.CONCLUSION:The optimized process is stable and feasible.

Extraction of Swertia chirayita(Roxb.ex Flemi) Karsten / 中成药

AIM:To determine the optimal extraction of effective component from Swertia chirayita(Roxb.ex Flemi) Karsten in Tibet. METHODS: HPLC method was used to determine the yield rate and mango glucoside content,the preference extract methods,extraction solvents and extraction method of Swertia chirayita(Roxb.ex Flemi) Karsten were chose by parallel comparative method. RESULTS: 75% ethanol was used to extract medicine materials by Percolate,the flow rate was 4 mL/min/kg,collecting 6 times liquor after percolate. CONCLUSION: The extraction method is simple and convenient,furthermore the extract stability is good too.

Orthogonal Design Experiment on the Extracting of Icariin by Different Means / 中国药房

OBJECTIVE:To optimize the extraction and preparation technics of icariin by orthogonal design.METHODS:The icariin was extracted by the ethanol reflux and the ethanol ultrasonic extracting process respectively;the quantity and concentration of ethanol,extracting time and extracting times were investigated with the content of the icariin as a indicator.RESULTS & CONCLUSION:The ethanol refluxing process is better than the ethanol ultrasonic process in the extraction of icariin.The most optimistic condition of extracting of icariin in ethanol refluxing extracting process was as follows:the crude drug was extracted for 3 times(40min/time)by using 60% ethanol,which is 8 times of the amount of the crude drug.