RESUMO
Aim With SD rats as control, to observe the anxiety susceptibility of FH/Wjd rats. Methods The anxiety behavior of 3-month-old SD rats and age-matched FH/Wjd rats were evaluated by elevated plus-maze test and open field test. The contents of 5-hydroxytryptamine (5-HT), dopamine (DA) and its metabolite from the cortex were detected by high performance liquid chromatography with electrochemical detection, and the metabolic ratios of DA and 5-HT were calculated. The activities of catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO), the contents of corticosterone (CORT) and adrenocorticotropic hormone (ACTH) were detected by enzymelinked immunosorbent assay in cortex. The expression of COMT was detected by polymerase chain reaction. The expression of all genes hippocampus was detected by mRNA-seq. Results As compared with SD rats, in FH/Wjd rats, the closed arms' distance and the total distance were significandy higher in elevated plus-maze; the central distance was significantly shorter, and the total distance was significantly longer in open field. The contents of DA,5-HT and DOPAC in cortex were significantly lower, and there was no significant difference in HVA and 5-HIAA. The ratio of HVA/DA and 5-HIAA/5-HT, the activity of COMT and MAO, the level of CORT and ACTH, the mRNA expression of COMTwere all higher. The differential genes of FH/Wjd rats and SD rats were mainly enriched in the neuroactive ligand-receptor interaction pathway. Conclusions Compared with SD rats, FH/Wjd rats have lower DA and 5-HT contents, hypermetabolism, hyperactivity of hypothalamic-pituitary-adrenal axis, abnormal expression of COMT and gene encoding neuropsychiatric system. Therefore, FH/Wjd rats have obvious anxiety characteristics.
RESUMO
Aim: To observe the preferences and drinking behavior characteristics of congenital alcoholism in (FH/Wjd) rats and SD rats. Methods: Both FH/Wjd rats and SD rats were fed with free access to two bottles (liquor A, liquor B, 53% alcohol and water). The intake of liquor and water and their preference, the difference in day and night intake of each liquor, and the variation of ethanol and water intake and the preference after depriving were compared between FH/Wjd rats and SD rats. Results: The rate of preference for liquor A was significantly higher than liquor B and 53% alcohol in FH/Wjd rats; however, the rate of preference for liquor B was significantly higher than that for liquor A and 53% alcohol, and the liquor A was higher than 53% alcohol in SD rats. It was found that the longer the drinking time, the more of alcohol consumption and the higher preference for each liquor in both FH/Wjd rats and SD rats. In addition, the drinking behavior of FH/Wjd rats and SD rats for each liquor showed a significant difference between day and night, with robust ethanol deprivation effect. Conclusions: SD rats can be forced to drink for short time, and prefer liquor B. However, FH/Wjd rats prefer liquor A. Both SD rats and FH/Wjd rats have the characteristics that the longer the drinking time, the more of alcohol consumption and the higher of preference. Obvious difference exists between day and night; the more alcohol consumption and higher rate of preference after deprivation appear in both SD rats and FH/Wjd rats.