RESUMO
Objective: To screen and isolate vascular endothelial growth factor (VEGF)-binding factors from Paris polyphylla var. yunnanensis, and explore their property for stimulating VEGF activity. Methods: The VEGF-binding factors from water extract of P. polyphylla var. yunnanensis were screened by VEGF-affinity chromatography and further purified by HPLC method. Their activity on proliferation of VEGF-dependent cells was determined by MTT analysis with sensitive cell line HepG2 as model. We also predicted the possible components according to RRLC-QTOF and UV results. Results: The VEGF-binding factors screened from the water extract of P. polyphylla var. yunnanensis were named as factor B3 which included three compounds and could induce the proliferation of VEGF-dependent cell line HepG2 but not the VEGF-independent cell line HEK293. Further studies indicated that factor B3 had an additive effect with VEGF to induce the proliferation of HepG2, and the additive effect could be attenuated by VEGF antibody. In addition, the proliferation of HepG2 cells induced by factor B3 alone could also be attenuated by VEGF antibody. Furthermore, based on the results of RRLC-QTOF and UV analysis, we predicted that factor B3 are probably members of saponin family. Conclusion: The factor B3 isolated from P. polyphylla var. yunnanensis has the property to stimulate VEGF activity.