RESUMO
Aim To study the role of Cx43 in inhibi-tion of AngII-induced vascular smooth muscle cells(VSMCs) proliferation by farrerol. Methods The primary VSMCs were isolated and cultured by direct adherent culture methods. VSMCs were identified by immunohistochemstry. The cells were divided into the following groups:control group,AngII group,AngII+Farrerol group. The cell viability was measured by CCK-8 cell vitality test. The proliferation of VSMCs was measured by the methods of Edu. The cell cycle of VSMCs was detected by flow cytometry. The mRNA levels of Cx43 were measured by Real-time PCR. The protein levels of Cx43 were measured by Western blot. Results 60 μmol·L-1farrerol could significantly de-crease the cell viability and EdU rate of VSMCs in-duced by AngII(P<0.05),which could also prevent the transformation of VSMCs from G0/G1phase to S phase. The results of real-time PCR and Western blot showed that,compared with the model group,Farrerol could significantly reduce the mRNA and protein ex-pression level of Cx43(P <0.01). After the interfer-ence of Cx43 by siRNA, the inhibition of proliferation by farrerol decreased significantly. Conclusion Far-rerol inhibits AngII-induced VSMCs proliferation signif-icantly, which might be associated with reducing the expression of Cx43.
RESUMO
Objective: To study the chemical constituents in the flowers of Rhododenron parvifolium, which is a traditional Tibet medicine. Methods: Silica gel column chromatography, HPLC, and Sephadex LH-20 column chromatography techniques were used for the separation and purification of the compounds. Their structures were determined on the basis of IR, MS, 1D, and 2D NMR spectral evidences. Results: Thirteen compounds were isolated from methanol extract in the flowers of R. parvifolium, and their structures were identified as parvifoliumol I (1), umbelliferone (2), scopoletin (3), 4', 5, 7-trihydroxyflavanone (4), 8-demethylfarrerol (5), farrerol (6), quercetin (7), rutin (8), eudesmin A (9), 4-hydroxybenzoic acid (10), 3, 5-dihydroxy toluene (11), oleanane (12), and kojic acid (13). Conclusion: Compound 1 is a new compound, which has a hexahydroxanthene skeleton. Compounds 9, 11, and 13 are obtained from the plants of Rhododendron L. for the first time.