Purification of Puerarin from Pueraria lobata by FCPC versus HSCCC Using Small-volume Columns / 中草药·英文版

Objective: To develop an efficient method for separating and purifying puerarin from the roots of Pueraria lobata. Methods: Separation by fast centrifugal partition chromatography (FCPC) was processed with a biphasic solvent system composed of ethyl acetate- n-butanol-water (2:1:3). The separation conditions were determined as follows: sample loading of 10 mg, flow rate of 2 mL/min, rotation speed of 2200 r/min, ascending mode, and detection wavelength of 254 nm. High speed countercurrent chromatography (HSCCC) was used as a comparative method with the rotation speed of 800 r/min, flow rate of 2.0 mL/min, and sample loading of 10 mg in tail-to-head mode. Results: Puerarin was obtained by FCPC with a resolution of 0.90 and a purity above 99%, while a resolution below 0.50 and a purity below 90% by HSCCC. Compared with HSCCC, FCPC has the advantages with higher purity and better resolution. Conclusion: FCPC is a powerful method to separate and purify puerarin. © 2014 Tianjin Press of Chinese Herbal Medicines.

Study on removing (-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids by fast centrifugal partition chromatography / 中草药

Objective: Using fast centrifugal partition chromatography (FCPC) to remove (-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids. Methods: The technological parameters of orthogonal experiment of FCPC was optimized by flow rate, rotation speed, and loading sample size. The optimized process was compared with the solvent extraction method, and the linear amplification experiments were carried out using the optimal process. Results: The optimized parameters were as follows: rotation speed was 1200 r/min, flow rate was 4 mL/min, and loading sample size was 80 mg/mL. The new prepared alkaloids fraction of Corydalis Decumbentis Rhizoma using amplification experiments has > 94.0% removed rate of BI and >85.0% recovery of the other alkaloids, which were higher than those of the solvent extraction method. This simple method with good repeatability could be completed in a short time. Conclusion: The simple and fast method could improve the safety of Corydalis Decumbens Rhizoma total alkaloids, and provide the industrialization of non-toxic total alkaloids.