Probiotic Potentials of Lactic Acid Bacteria Isolated from Fermented Foods

This study investigated the probiotic potentials of seven lactic acid bacteria (LAB) strains at different temperatures, pH, and bile salt concentrations. Their antimicrobial activity and antibiotic susceptibility were also determined. There were significant (P˂0.05) differences in the LAB growth at 45-650C with viable counts ranging from 4.28-8.34 Log10 Cfu/ml after 48 h. The LAB strains showed significant (P˂0.05) increase at pH 2, 2.5 and 3 after 3 and 6 h. L. parabuchneri LMG was viable at 45 and 65oC with 99.30 and 65.00% survival respectively. The LAB showed high resistance to 0.3% bile salt at 97.90%. L. plantarum CIP was viable with 95.40% survival at pH 3.0 after 3 h. All the LAB strains were susceptible to cefuroxime (20 µg/ml) and erythromycin (10 µg/ml) at 13.00-45.00 mm zone of inhibition (ZOI). They had strong antimicrobial activity against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027 and Listeria monocytogenes ATCC 15313. Leuconostoc mesenteroides LM and L. brevis ATCC inhibited the five tested food borne pathogens with ZOI varying from 8.00-26.00 mm. The results from this study showed that the LAB strains isolated from fermented foods had probiotic potential and can be used for research and commercial purposes.

Avaliação da interação entre leveduras isoladas de kombucha e bactérias probióticas / Evaluation of the interaction between isolated kombucha yeasts and probiotic bacteria

Um dos maiores desafios no desenvolvimento de produtos probióticos é entender como os microrganismos interagem entre si e com o hospedeiro. Quando falamos em alimentos fermentados tradicionais, este obstáculo aumenta porque a matriz alimentar já possui um microbioma intrínseco. No entanto, também é conhecido que muitos microrganismos podem interagir e cooperar para sobreviver quando condições de estresse são encontradas. Assim, o objetivo deste trabalho foi isolar leveduras de quatro diferentes kombuchas em distintos momentos fermentativos e verificar a influência que leveduras isoladas de kombucha têm na manutenção da viabilidade da bactéria probiótica Bifidobacterium animalis subsp. lactis HN019 em condições de aerobiose. Meyerozyma guilliermondii, Candida albicans, Rhodotorula mucilaginosa e Pichia membranifaciens foram leveduras encontradas nas kombuchas, das quais as duas últimas favoreceram a manutenção da alta viabilidade de HN019 em cocultura por 14 dias. Observou-se a viabilidade da bactéria acima de 9 log ao longo de todo o experimento, o que não foi observado em monocultura. Ademais, utilizou-se de análise de autoagregação, hidrofobicidade, atividade enzimática de proteases e fosfolipases das leveuras para analisar seu potencial patogênico. Observou-se que R. mucilaginosa demonstrou características semelhantes à Saccharomyces cerevisiae subsp. boulardii, e sua interação benéfica com HN019 reforça a possibilidade de que esta levedura seja uma chave para a inserção da bactéria em uma kombucha probiótica. Análises metabólicas foram realizadas e encontrou-se uma vasta diversidade de dipeptídeos, principalmente os compostos de prolina, durante a cocultura da bactéria com as leveduras. Tais dipeptídeos apresentam importantes mecanismos de ação no controle biológico e quorum sensing de bactérias e leveduras, e supostamente regulam a manutenção das relações mutualísticas entre ambos microrganismo

One of the biggest challenges in the development of probiotic products is to understand how microorganisms interact with each other and with the host. When we talk about traditional fermented foods, this obstacle increases because the food matrix already has an intrinsic microbiome. However, it is also known that many microorganisms can interact and cooperate to survive when stressful situations are encountered. Thus, the objective of this work was to isolate yeasts from four different kombuchas at different fermentation times and to verify the influence that yeasts isolated from kombucha have on maintaining the viability of the probiotic bacterium Bifidobacterium animalis subsp. lactis HN019 under aerobic conditions. Meyerozyma guilliermondii, Candida albicans, Rhodotorula mucilaginosa and Pichia membranifaciens were yeasts found in kombuchas, of which the last two favored the maintenance of HN019 high viability in co-culture for 14 days. Bacteria viability above 9 log was observed throughout the experiment, which was not observed in monoculture. In addition, analysis of autoaggregation, hydrophobicity, enzyme activity of proteases and phospholipases of yeasts was used to analyze their pathogenic potential. It was observed that R. mucilaginosa demonstrated characteristics similar to Saccharomyces cerevisiae subsp. boulardii, and its beneficial interaction with HN019 reinforces the possibility that this yeast is a key to the insertion of the bacterium in a probiotic kombucha. Metabolic analysis were performed and a wide diversity of dipeptides, mainly proline-based, was found during the co-culture of the bacteria with the yeasts. Such dipeptides have important mechanisms of action in the biological control and quorum sensing of bacteria and yeast, and supposedly regulate the maintenance of mutualistic relationships between both microorganism

Kombucha como alimento potencialmente psicobiótico / Kombucha as a potential psychobiotic food

Os casos de transtorno de ansiedade têm apresentado crescimento considerável desde o início do século XX, onde a terapia medicamentosa oferecida, geralmente apresenta efeito sedativo, portanto, a busca por tratamentos adjuvantes para tratar quadros de ansiedade se fazem necessários. Estudos indicam que a modulação da microbiota intestinal pode estar relacionada à regulação neural dos indivíduos através de diversas vias, incluindo a aplicação de cepas probióticas e consumo de alimentos fermentados tradicionais como iogurte e kombucha, colaborando para a melhoria da qualidade de vida destes pacientes. Este projeto teve como objetivo buscar os metabólitos e neurotransmissores presentes no kombucha a fim de verificar seu potencial psicobióticos e comparar as aplicações e metabólitos produzidos por cepas probióticas existentes no mercado e em alimentos fermentados tradicionais que atuem no eixo intestino-cérebro. Foram realizadas pesquisas em bases de dados online, como Pubmed, Web of Science, Scielo, Scopus e Google Scholar no período entre 2002 e 2022 relacionados aos possíveis efeitos dos probióticos em condições de ansiedade, bem como como os mecanismos que envolvem o eixo cérebro-intestino, seja por meio de testes em humanos e em modelos animais. As espécies mais testadas quanto ao seu potencial probiótico e ação nos transtornos de ansiedade encontradas foram Lactobacillus paracasei, L. casei, L. rhamnosus, Bifidobacterium infanti e B. longum. Cada gênero demonstra um grau diferente na redução da ansiedade dos indivíduos. Os alimentos potencialmente probióticos, incluindo alimentos fermentados tradicionais, além de atuar como complemento à terapia em quadros de ansiedade, tem relevância no setor socioeconômico

Anxiety disorder cases have shown considerable growth since the beginning of the 20th century, where the drug therapy offered usually has a sedative effect. Therefore, the search for adjuvant treatments to treat anxiety disorders is necessary. Studies indicate that the modulation of the intestinal microbiota may be related to the neural regulation of individuals in several ways, including the application of probiotic strains and consumption of traditional fermented foods such as yogurt and kombucha, contributing to the improvement of the quality of life of these patients. This project aimed to identify and compare the psychobiotic effect in the gut-brain axis of the metabolites and neurotransmitters produced by kombucha and commercial probiotic strains. The research was carried out in online databases, such as Pubmed, Web of Science, Scielo, Scopus, and Google Scholar in the period between 2002 and 2022 related to the possible effects of probiotics in anxiety conditions as the mechanisms that involve the brain-gut axis either through tests in humans or animal models. The species most tested for their probiotic potential and action on anxiety disorders were Lactobacillus paracasei, L. casei, L. rhamnosus, Bifidobacterium infanti, and B. longum. Each genus demonstrates a different degree of reducing individuals' anxiety. Potentially probiotic foods, including traditional fermented foods, acting as a complement to therapy in cases of anxiety, have relevance in the socioeconomic sector

Alimentos fermentados y probióticos en niños. La importancia de conocer sus diferencias microbiológicas / Fermented foods and probiotics for children. The importance of knowing their microbiological differences

La leche materna provee microorganismos que colonizan el intestino y programan el sistema inmunológico para desarrollar tolerancia oral. Entre los 6 meses de lactancia materna exclusiva y los 2 años de lactancia prolongada recomendada, la alimentación complementaria conlleva una reducción progresiva en el ingreso de microorganismos vivos al ecosistema intestinal. Esto se debe a que los alimentos en general -a diferencia de la leche materna- o se encuentran desprovistos de microorganismos o, si los poseen, suelen inactivarse durante la cocción. Los alimentos fermentados y los probióticos podrían constituir una estrategia nutricional valiosa, dado que garantizarían la provisión de microorganismos vivos ante la reducción o interrupción anticipada de la lactancia. Los términos "alimentos fermentados" y "probióticos" no son sinónimos. La identidad microbiológica, la inocuidad y la existencia de estudios clínicos de eficacia para unos y otros son claves para entender sus diferencias y decidir una eventual recomendación alimentaria

Breast milk provides microorganisms that colonize the gut and program the immune system to develop oral tolerance. Between the 6 months of exclusive breastfeeding and the recommended 2 years of prolonged breastfeeding, complementary feeding leads to a progressive reduction in the entry of live microorganisms into the gut ecosystem. This is because foods in general -unlike breast milk- are devoid of microorganisms or, if present, they are often inactivated during cooking. Fermented foods and probiotics could be a valuable nutritional strategy, as they would ensure the supply of live microorganisms in the face of a reduction or early cessation of breastfeeding. The terms "fermented foods" and "probiotics" are not synonymous. Microbiological identity, safety, and the existence of clinical efficacy studies supporting both are key to understand their differences and decide on an eventual dietary recommendation

Veracidade da rotulagem quanto a presença de amido baseado em um teste laboratorial em iogurtes nacionais comercializados no sul de Santa Catarina / Veracity of labeling as the presence of starch based on a laboratory test in national yogurts commercialized in southern Santa Catarina

Com o crescimento diário do número de consumidores em nível mundial, a indústria alimentícia se caracteriza como um ramo em ascensão contínua. Como consequência, a sociedade procura obter informações nutricionais dos produtos consumidos por meio dos rótulos. O iogurte, objeto do presente estudo, é tido pela sociedade de consumo como um alimento rico em nutrientes, que promove benefícios ao organismo e, por isso, apresenta-se como vantajoso à saúde. No entanto, torna-se fundamental analisar a veracidade nos rótulos desses produtos quanto à presença de amido. Tal análise pode ser efetivada por meio de um exame laboratorial chamado de teste de iodo, também conhecido como teste Lugol. Com isso, o presente estudo objetivou analisar a veracidade da rotulagem quanto à presença de amido, baseando a pesquisa em um teste laboratorial em iogurtes nacionais comercializados no sul de Santa Catarina. Sendo assim, os resultados das 28 amostras deste estudo demonstraram alguma inconformidade. Observou-se que 85,7% apresentavam amido declarado como espessante na rotulagem, porém o teste foi positivado para 89,3% dos iogurtes. Diante disso, são de suma importância as análises na veracidade das rotulagens, visto que, corriqueiramente, o consumidor é lesado ao que diz respeito às informações contidas nos rótulos de produtos alimentícios. (AU)

With the daily growth of the number of consumers worldwide, the food industry is characterized as a branch in continuous growth. As a result, society seeks to obtain nutritional information on the products consumed through the labels. Yogurt, the object of this study, is considered by the consumer society as a food rich in nutrients, which promotes benefits to the body and, therefore, presents itself as advantageous to health. Such an analysis can be carried out through a laboratory test called iodine test, also known as Lugol test. Whithin this context, the present study aims to analyze the veracity of the labeling regarding the presence of starch by testing national yogurts commercialized in the south of Santa Catarina. Thus, the results of this study showed that samples were not in conformity. It was observed that 85.7% had starch declared as a thickener on the label, but the test was positive for 89.3% of yogurts. Therefore, it is of utmost importance to analyze the veracity of the labeling, once routinely, the consumer is harmed when it comes to the information contained in the labels of food products. (AU)

Bio-preservative Activities of Partially Purified Bacteriocin Extracts of Lactobacillus mindensis TMW and Lactobacillus tucceti CECT 5920 Isolated from Nigerian Fermented Foods

The preservative potentials of lactic acid bacteria (LAB) recovered from some Nigerian traditional fermented foods were assessed by inoculating 0.1 ml aliquots of suitable dilutions of the food samples on De Man Rogosa Sharpe (MRS) agar fortified with 50mg of nystatin. The isolates were assessed for their ability to produce bacteriocin using Agar Well Diffusion assay method. By using (GTG)5-PCR and 16s rDNA sequencing tools, two bacteriocin-producing LAB namely Lactobacillus tucceti CECT 5920 and Lactobacillus mindensis TMW were identified. Both LAB isolates had equal level of bacteriocin activity. Studies on inhibitory activity of partially purified bacteriocin extracts showed that temperature of 35°C had maximum effect on bacteriocin antimicrobial activity of L. tucceti CECT 5920 against Staphylococcus aureus NCTC 8325 and Escherichia coli 0157:H7, while pH had same effects on both LAB isolates against the test bacteria. Maximum tolerance of acidic medium was exhibited by both LAB isolates at pH 3-8. Bacteriocin inhibitory activity was best against both test bacteria at 0.2% concentration of NaCl. Combination of ginger with partially purified bacteriocin sample from both LAB isolates had reduction effect on both test pathogens for both Lactic acid bacteria isolates. When stored for 14 days, bacteriocin activity was optimum for the first day against both test pathogens, but decreased progressively with increased in storage time. The two LAB isolates had similar inhibitory activity against both test pathogens. L. tucceti CECT 5920 gave greater reduction result (90.03%) on S. aureus NCTC 8325 in fish sample and 78.07% against E. coli 0157:H7 in meat sample while L mindensis TMW had highest significant reduction effect (90.12%) in fish sample against S. aureus NCTC 8325 and 77.80% against E. coli 0157:H7 in fish sample. Both LAB isolates will perform well as preservative agents in food preservation as they showed production of bacteriocin which has antimicrobial activity and rapid acidification in growth medium.

Identification and characterization of lipolytic bacteria from Thai fermented foods

Aims: Gram-positive spore forming rod-shaped bacteria from Thai fermented foods were isolated, identified and screened for lipolytic activity. Methodology and results: Bacterial strains were isolated from Thai fermented foods by the standard dilution technique using Tryptic soy agar. Seven isolates which belong to the genus Bacillus and one isolate which belongs to Paenibacillus were characterized based on their phenotypic characteristics and 16S rRNA gene sequence analyses. Isolates NM8-1 (Group 1), PR9-2 (Group 2), PR11-1 (Group 3), KM15-1 and SS49-4 (Group 4), SS48-5 (Group 5) and SS503 (Group 6), were closely related to Bacillus methylotropicus, B. pumilus, B. flexus, B. cereus, B. subtilis and B. anthracis, based on 99.90-100% similarities, respectively. Isolate NM45-3 (Group 7) was closely related to Paenibacillus pasadenensis based on 99.55% similarity. All the isolates were susceptible to amikacin, chloramphenicol, clindamycin, erythromycin, gentamicin, imipenem, kanamycin, levofoxacin, novobiocin, streptomycin, tetracycline and vancomycin. Their lipase production in nutrient broth with Tween 20, Tween 40, Tween 60 or Tween 80 ranged from 0.014±0.129 - 3.231±0.087 U/mL. Bacillus subtilis SS48-5 exhibited highest lipase production when cultured with Tween 80 at pH 7.5 for 24 h. The optimum lipase production of this strain was at 40 °C after incubated for 30 h. Conclusion, significance and impact of study: The characterization and evaluation of the lipolytic activity of Bacillus and Paenibacillus strains isolated from Thai fermented foods will be useful for the species diversity, food fermentation and the lipase production.

Vitamin B12 Contents in Some Korean Fermented Foods and Edible Seaweeds

There is a limitation to estimate vitamin B12 intake due to lack of data on vitamin B12 content in many Korean foods. In this study, vitamin B12 content was determined in some soybean or vegetable-fermented foods, edible seaweeds and other frequently consumed foods in Korea by microbioassay using Lactobacillus delbruecki ATCC 7830. The traditional type of Doenjang and Chungkookjang contained 1.85 microgram/100 g and 0.69 microgram/100 g of vitamin B12, respectively, while the factory-type of Doenjang and Chungkookjang contained 0.04-0.86 microgram/100 g and 0.06-0.15 microgram/100 g. Vitamin B12 was not detected in steamed soybeans and Tofu which is a not-fermented soybean product, indicating that vitamin B12 in Doenjang and Chungkookjang might be produced during the fermentation process. The Korean-style soy sauce contained 0.04 microgram vitamin B12/100 mL, but vitamin B12 was not detected in Japanese-style soy sauce and white miso. Commercial Kimchi, a representative Korean vegetable- fermented food, made of Korean cabbage, Yeolmu, or Mustard leaves contained 0.013-0.03 microgram vitamin B12/100 g, while Kimchi without red pepper and fermented fish sauce (White Kimchi) did not. Vitamin B12 content was very high in some edible seaweeds such as laver (66.76 microgram/ 100 g dry weight) and sea lettuce (84.74 microgram/100 g dry weight), and it was 17.12 microgram/100 g of dried small anchovy, 1.07 microgram/100 g of whole egg, and 0.02 microgram/100 g of coffee mix. From these results, it is assumed that Koreans take substantial amount of vitamin B12 from plant-origin foods. And, with these data, we will be able to calculate dietary vitamin B12 content more correctly than before. In conclusion, soybean-fermented foods, Kimchi, laver and sea lettuce are recommendable as good sources of vitamin B12 for vegetarians or Korean elderly on grain and vegetable based diet.

The effect of fermented foods on the color change of soft denture liners / 대한치과보철학회지

STATEMENT OF PROBLEM: The clinical criteria of soft lining materials are resilience over an extended period, capability of forming a strong bond with denture base materials, dimensional stability, adequate tear strength, and color stability. Many researches and reports dealt with food colorants or denture cleanser, but not with fermented foods. PURPOSE: This study was designed to assess what fermented foods, such as soy sauce and red pepper paste that many Koreans have eaten, influence on the color stability of soft denture liners. MATERIAL AND METHODS: The color differences(delta E*) were measured by spectrophotometer with different immersion time. For the procedure, thirty disk-shape specimens per 4 soft denture liners(Molloplast-B(R), Ufi Gel SC(R), Dura Base(R), Sofreliner MS(R)) were fabricated with a thickness of 2mm and 16mm in diameter. Each 10 specimen were immersed into the beakers of fermented foods and distilled water, and L*, a*, and b* values were measured for the color difference(delta E*), on the 1st, 7th, and 28th day with spectrophotometer. RESULT AND CONCLUSION: 1. There were significant differences between samples(soy sauces, red pepper pastes, and distilled water) in Sofreliner MS(R) of 1st day after immersion(p<0.05). There were significant differences between samples in Sofreliner MS(R) and Ufi Gel SC(R) of 7th days after immersion(p<0.05). There were significant differences between samples in Molloplast-B(R) of 28th day after immersion(p<0.05). 2. In red pepper pastes, delta E* values of Molloplast-B(R), Ufi Gel SC(R), Sofreliner MS(R) were higher than 3.3. Those values were not clinically acceptable. In soy sauces, delta E* values of all denture liners were lower than 3.3. delta E* values of Ufi Gel SC(R) were higher than those of other denture liners. 3. Based on the above results, red pepper paste causes more discoloration than soy sauce.

The effect of fermented foods on the color and hardness change of denture base acrylic resins / 대한치과보철학회지

STATEMENT OF PROBLEM: For a long time, many of denture base acrylic resins have been used for edentulous and partial edentulous patients because of easy manipulation and good mechanical properties, but its esthetic aspect has not been commented enough. Denture base acrylic resins also has caused esthetic problems due to discoloration or staining as in esthetic restoration. Many researches and reports have treated the problems and accomplished esthetic improvement. But these researches and reports dealt with general food colors or beverages, not with fermented foods. PURPOSE: This study is designed to assess what fermented foods, such as soy sauce, gochujang, and toenjang that many of Koreans have taken in, influence on the color and hardness variation of denture base acrylic resins. MATERIALS AND METHODS: For the procedure, twelve disks per 4 denture base acrylic resins were fabricated with a thickness of 2mm and 16mm in diameter. Each seven specimen were measured for discoloration with spectrophotometer, while the others, five specimen, for surface hardness change with Barcol hardness tester, over time. Each 12 specimen were immersed into the 4 beakers of fermented foods(soy sauces, gochujangs, toenjangs, deionized water), and L*, a*, and b* values were measured for the color difference(_E*), on the 1st, 7th, and 28th day with spectrophotometer, with the measurement of surface hardness change. Each data observed was processed statistically. RESULTS: The findings are as follows; Discoloration 1. All of denture base resins was not influenced by the kind of fermented foods, except for QC20. 2. Soy sauce and red pepper paste caused more change for denture base resins than deionized water and soy bean paste, except for Perform. 3. Most significant change was shown in Lucitone199., whereas Perform. results in the least change for all immersed solution, with no statistical significance. Hardness change 1. Barcol hardness values in deposited specimens have been changed low degree, but with significant statistical change according to the kind of food and duration. 2. Lucitone199. has significantly lower Barcol hardness value than others do. CONCLUSION: Based on the above results, it suggests that the habitual intake of fermented foods is not helpful for the color stability of denture base acrylic resins because Soy sauce and red pepper paste mainly caused discoloration and surface hardness change. Particularly Lucitone199. shows specific discoloration and low surface hardness values. Therefore, it is recommended giving caution patients with denture of Lucitone199. especially against the habitual intake of fermented foods like soy sauce and red pepper paste.

Nutritional Evaluation of Traditional Fermented Foods by Dietary Intake of University Students in Chungnam

The purpose of this study was to evaluate nutritional value of traditional fermented foods by dietary intake of university students living in Chungnam. 210 students were asked for their daily food intake using 24-hour recall method for 3 days. And daily nutrient intakes from total foods and fermented foods were analyzed and compared. The mean weight, height, and BMI of the subjects were 67.36kg, 174.39cm, 22.15kg/m2 in men and 53.45kg, 161.86cm, 20.40kg/m2 in women, respectively. The daily intakes of protein, phosphorus, and vitamin B1 were 106.82%, 137.88%, and 108.99% of the Korean RDA. However, the energy, calcium, iron, vitamin A, vitamin B2, niacin, vitamin C intakes did not reached the RDA for Koreans. The fermented food intake was 9.09% of total food intake. And the percentages of fermented food intakes were 81.53% for Kimchi and 14.75% for Chang. The vegetable protein, vegetable oil, dietary fiber, calcium, phosphorus, iron, sodium, potassium, vitamin A, vitamin B1, vitamin B2, niacin and vitamin C intakes per 1000kcal consumption from fermented foods were significantly higher than those from total foods. The fermented food intake showed positive correlation with iron, and plant iron intakes. In conclusion, the percentage of fermented food intake was about 9% and the major items were Kimchi and alcoholi beverages in university students. Fermented food intake may be important to improve the intakes of calcium, iron and vitamin.

Nutritional Evaluation of Traditional Fermented Foods by Dietary Intake of University Students in Chungnam

The purpose of this study was to evaluate nutritional value of traditional fermented foods by dietary intake of university students living in Chungnam. 210 students were asked for their daily food intake using 24-hour recall method for 3 days. And daily nutrient intakes from total foods and fermented foods were analyzed and compared. The mean weight, height, and BMI of the subjects were 67.36kg, 174.39cm, 22.15kg/m2 in men and 53.45kg, 161.86cm, 20.40kg/m2 in women, respectively. The daily intakes of protein, phosphorus, and vitamin B1 were 106.82%, 137.88%, and 108.99% of the Korean RDA. However, the energy, calcium, iron, vitamin A, vitamin B2, niacin, vitamin C intakes did not reached the RDA for Koreans. The fermented food intake was 9.09% of total food intake. And the percentages of fermented food intakes were 81.53% for Kimchi and 14.75% for Chang. The vegetable protein, vegetable oil, dietary fiber, calcium, phosphorus, iron, sodium, potassium, vitamin A, vitamin B1, vitamin B2, niacin and vitamin C intakes per 1000kcal consumption from fermented foods were significantly higher than those from total foods. The fermented food intake showed positive correlation with iron, and plant iron intakes. In conclusion, the percentage of fermented food intake was about 9% and the major items were Kimchi and alcoholi beverages in university students. Fermented food intake may be important to improve the intakes of calcium, iron and vitamin.