Expression of FN1 in gastric cancer and its clinical significance / 医学研究生学报

Objective Fibronectin 1 (FN1) is a glycoprotein involved in cellular adhesion and migration processes. The aim of this study was to investigate the expression and clinicopathological significance of FN1 in gastric cancer and to predict the possible mechanism of FN1. Methods GEO data and TCGA data were downloaded. FN1 expression in gastric cancer and adjacent tissues was analyzed by GSE54129 data, and then verified by GSE29272 and TCGA data. According to the expression profile data and FN1 expression, mRNA expression value was divided into low expression(<-0.475), and medium expression(-0.475~1.036), high expression (>1.036). FN1 expression in gastric cancer and clinicopathological relationship were analyzed. TCGA data and Kaplan Meier were used to analyze the relationship between the expression level of FN1 and the prognosis of gastric cancer patients; while gene concentration analysis (GSEA) was used to predict the related path of FN1. Results TCGA data showed the medium survival time of low, medium, high FN1 expression was respectively 63.5, 55.7, 39.4 months, and the difference between low expression and high expression in survival time was of statistical significance. Kaplan Meier Plotter online data analysis showed the medium survival time of FN1 high expression was shorter than that of low expression(P<0.01), which meant the higher the FN1 expression was, the worse the prognosis was. FN1 expression is an independent prognostic factor (HR=0.480,95% CI:0.336~0.686). High expression of FN1 samples enriched gene sets such as KRAS (FDR=0.052), P53(FDR=0.052), TGF-β(FDR=0.052), cell adhesion(FDR=0.0), extracellular matrix (FDR=0.043)and cytoskeletal protein regulation (FDR=0.052). Conclusion The high expression of FN1 is a poor prognostic factor for gastric cancer and can be used as an effective biomarker for predicting the metastasis and prognosis of gastric cancer. High expression of FN1 leads to abnormalities in KRAS, P53, TGF-β, ECM, cell adhesion and cytoskeletal protein regulatory pathways.

Expression of survivin, fibronectin-1, VEGF and ezrin in thyroid tumors and their relationship with tumor pathological characteristics / 中国肿瘤生物治疗杂志

@# Objective: To investigate the expression of survivin, fibronectin-1, vascular endothelial growth factor (VEGF) and ezrin in thyroid tumors and their relationship with the pathological characteristics of thyroid tumors. Methods: Ninety patients with thyroid tumors admitted to the third affiliated hospital of Zunyi Medical University and the first hospital during Oct. 2016 and Oct. 2018 were selected as the observation group. Seventy-five patients with normal thyroid confirmed by pathology in the same period were selected as the control group. The protein levels of survivin, fibronectin-1, VEGF and ezrin were detected by immunohistochemical method. Results: The positive rates of survivin, fibronectin-1, VEGF and Ezrin in the control group were 2.67%, l4.00%, 1.33% and 1.33%, which were lower than 97.78%, 96.67%, 93.33% and 95.56% in the observation group, respectively (all P<0.05 or P<0.01). The expressions of survivin, fibronectin-1, VEGF and ezrin were significantly correlated with TNM staging, tumor diameter, extrathyroid invasion and lymphatic metastasis (all P<0.05). Conclusion: Survivin, fibronectin-1, VEGF and ezrin proteins are all involved in the occurrence and development of thyroid tumors. The combined detection of these four indicators is of great significance in the diagnosis, treatment and prognosis of thyroid tumors.

Proteomic analysis of serum exosomes derived from Crouzon syndrome patients carrying a mutant FGFR2C342Y / 中华整形外科杂志

Objective@#Reveal the global expression profile of serum exosomal proteins of Crouzon syndrome patients.@*Methods@#We isolated microvesicles from serum of Crouzon children with a C342Y mutation in FGFR2 by ultracentrifugation, which were further characterized by electron microscopy and immunoblotting. The protein profiling in normal subjects and Crouzon patients was systematically compared by iTRAQ proteomic analysis.@*Results@#The result demonstrated that microvesicles were between 30—100 nm in diameter, round shape with cup-like concavity and expressed exosomal marker tumor susceptibility gene (TSG) 101 and flotillin (Flot) 1. We identified a total number of 62 proteins, among which 22 proteins overlap with ExoCarta database and were different between the Crouzon patient and the normal subject. The Ingenuity Pathway Analysis showed that the functions of these proteins are mostly involved in Developmental Disorder, Hereditary Disorder, Skeletal and Muscular Disorders, which are all related to the clinical manifestations of Crouzon syndrome. In addition, the proteins were focused on the network of "Organismal Injury and Abnormalities, Hematological System Development and Function, Cell-To-Cell Signaling and Interaction" . The central protein FN1 was presented as the key protein in the network.@*Conclusions@#Our data demonstrated that serum exosomes harbor informative proteins and FN1 was selected as a potential candidate for its role in promoting osteoblast adhesion, proliferation and mineralization.

THE PLASMA FIBRONECTIN AND ?1-ANTITRYPSIN IN DIABETES MELLITUS / 中华内分泌代谢杂志

The plasma fibronectin (Fn) and ?1-antitrypsin (?1-AT) in 25 diabetic patients (NIDDM) were determined with a rate nephelometry. The results showed that the plasma Fn in the diabetic patients was obviously lower (P